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Histology - Katie > Exam 2 clicker ?'s > Flashcards

Exam 2 clicker ?'s Flashcards

1
Q

Parathyroid hormone stimulates osteoblasts to secrete/express which of the following?

A. Osteoprotegerin - synthesis is stopped by presence of parathyroid hormone

B. M-CSF

C. RANKL

D. All the above

E. Only B and C

A

E. Only B and C

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2
Q

Which of the following is not part of a Haversian system?

A. Periosteum

B. Lacunae

C. Lamellae

D. Volkmann canals

E. Canaliculi

A

A. Periosteum

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3
Q

Which of the following is NOT a characteristic of fibrocartilage?

A. Type II collagen

B. Not surrounded by perichondrium

C. Found in intervertebral discs

D. Increases collagen in the matrix

E. Neither A or B are characteristic of fibrocartilage

A

A. Type II collagen

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4
Q

ID this Tissue

A

Dry Ground Bone 400x

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5
Q

ID this Tissue and Labeled structures

A

Bone (dry Ground) 400x

A. Canaliculi

B. Lamellae

C. Haversian Canal

D. Lacuna

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6
Q

ID this Tissue

A

Dry Ground Bone 100x

Dark = air staining

Light = no air staining

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7
Q

ID this tissue

A

Bone (Dry Ground) 100x

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8
Q

ID this tissue

A

Developing Membrane Bone (fetus) 400x

A. Osteoblasts

B. Osteocytes

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9
Q

ID this tissue

A

Developing Membrane Bone (fetus) 100x

a. Osteoblasts

B. osteocytes w. some semblance of lacunae

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10
Q

ID this tissue

A

Developing Membrane Bone (fetus) 40x

A. Lots of Blood vessels

B. Trabeculae

C. Osteoblast

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11
Q

ID this tissue

A

Cancellous Bone 400x

A. Osteocytes

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12
Q

ID this tissue

A

Cancellous Bone, 100x

A. Osteoblasts

laying bone on top of existing material so appositional growth

looks like epithelium but single layer of osteoblast laying down bone

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13
Q

ID this tissue

A

Cancellous (Spongy) Bone 40x

A. Periosteum

B. Trabeculae

C. Osteogenic Connective tissue (the lighter stained area CT signaled to start forming bone

D. Hematopoietic Tissue - tissue that consists of lots of BC in various stages

sinusoids connected to vascular systems allows RBC to escape to the blood system

Really looking at the organic matrix of bone. dissolved the inorganic matrix, if looked at different magnification would see more holes

skeletal muscle in left side

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14
Q

Label and ID

A

Compact Bone

A. Fibrous periosteum - CT layer (outer portion)

B. Osteogenic periosteum - inner portion of star shaped appearane

C, Lacuna -star shaped due to osteocytes inside

D. Lamellae

E. Canaliculus

F. Haversian Canal - some nerve endings little cytoplasmic processes that interject with others a little farther out

G. Volkmann Cannal

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15
Q

ID this tissue

A

Fibrocartilage H&E

A. Fibrous Cartilage

B. Condrocytes

C. Fibrous Matrix

don’t really see collagen fibers but the key to look for are the strings of chondrocytes that are being ordered in tension

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16
Q

ID this tissue

A

Fibrocartilage

don’t really see collagen fibers but the key to look for are the strings of chondrocytes that are being ordered in tension

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17
Q

ID the Tissue

A

Elastic Cartilage

the matrix here see network appearance of fibers. They run in different directions.

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18
Q

ID the Tissue

A

Elastic Cartilage

The matrix here see network appearance of fibers. They run in different directions

This is high magnification

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19
Q

ID the Tissue

A

Hyaline Cartilage

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20
Q

ID the Tissue

A

Hyaline CArtilage 400x

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21
Q

ID the Tissue

A

Hyaline Cartilage 400x

A. Chondrocyte in Lacuna

B. Territorial Matrix

C. Interterritorial Matrix

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22
Q

ID the Tissue

A

Hyaline Cartilage, 100x from the trachea

A. Fibroblasts of Perichondrium

B. Young Chondroblasts

C. Isogenous groups of chondrocytes

have appositional growth (blue arrow to right)

Interstitial Growth (left blue arrow)

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23
Q

Id diagram and Labels

A

Hyaline Cartilage

a. Fibrous perichondrium (source of fibroblasts)

B. Chondrogenic perichondrium (source of chondroblasts and osteoprogenitor cells)

C. Lacuna

D. Chondrocytes

E. Matrix

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24
Q

ID tissue and labels

A

Epiphyseal Plate

A. Proliferative Zone

B. Hypertrophic Zone

C. Calcificaton of Territorial Matrix

D. Osteoblasts (right on top of calcified matrix which is where you would expect them

E. Osteoid Front -

Diaphysis on Left

Epiphysis on right

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25
Q

ID tissue

A

Joint :Fetal Finger

A. Synovial Cavity

B. Articular Cartilage - All chondrocytes

C. Synovial Projection - just CT nothing to do w/ formation of joint

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26
Q

ID tissue

A

Joint: Fetal Finger (100x)

A. Zone of Ossification

B. Zone of Hypertrophy

C. Zone of Calcification

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27
Q

ID tissue

A

Joint: Fetal Finger (40x)

A. Zone of Ossification

B. Zone of hypertrophy

C. Zone of Calcification

D. Synovial Cavity

Arrow indicates replacement of cartilage to bone

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28
Q

ID tissue

A

Developing Membrane Bone (fetus) 400x

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29
Q

ID tissue

A

Developing Membrane Bone (fetus) 100x

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30
Q

ID tissue

A

Developing Membrane Bone (fetus) 40x

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31
Q

ID tissue

A

Cancellous Bone 400x

A. Blood Vessel

B. Osteoblasts

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32
Q

ID tissue

A

Cancellous Bone, 100x

A. Osteoblasts

B. Blood Vessels

C. Osteoblasts: Monolayer

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33
Q

ID tissue

A

Cancellous Bone, 40x

A. Trabecula

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34
Q

ID

A

Sickle Cell Erythrocytes

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35
Q

ID

A

’s increase under the influence of erythropoietin

Normal Erythrocytes

Light center = flattened biconcave discs = center flattened more than outside

devod of granules and organelles (once mature)

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36
Q

ID

A

Neutrophils 1000x

1,2,3 get the appearance of granules

7-9 mircrometers

3-5 nuclear lobes with connecting strands

active ameboid phagocytes

small, numerous specific granules

large, less numerous azurophilic granules

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37
Q

ID

A

Neutrophils 1000x

  1. Neutrophil; 5 lobes
  2. bar body

means it’s female

7-9 mircrometers

3-5 nuclear lobes with connecting strands

active ameboid phagocytes

small, numerous specific granules

large, less numerous azurophilic granules

38
Q

ID

A

Neutrophils 1000x

7-9 mircrometers

3-5 nuclear lobes with connecting strands

active ameboid phagocytes

small, numerous specific granules

large, less numerous azurophilic granules

39
Q

ID

A

Neutrophils 1000x

7-9 mircrometers

3-5 nuclear lobes with connecting strands

active ameboid phagocytes

small, numerous specific granules

large, less numerous azurophilic granules

40
Q

ID

A

Eosinophil

9-10 micrometers

color-enhanced (probably)

difficult to see the nucleus but can clearly see the granules in both

bilobed nucleus

0.5% make up

41
Q

ID

A

Basophil

7-9 micrometers

very difficult to see the nucleus but can clearly see the granules

Lobulated nucleus (bilobed)

Large, membrane bound basophilic granules

stain blue to dark purple and are very large

Basophils are a little more common thant eosinophils

42
Q

ID

A

Lymphocyte 1000x

Most of the cell taken up by the nucleus so gives us a crescent shaped cytpnucleus on one side

Large nuclei make easiest to identify

Large Round

have 2 kinds B lymphoctes and T lymphoctes

43
Q

ID

A
  1. Monocyte
    - not as common as neutrophils or lymphocytes but still common

largest leukocyte

kidney shaped nucleus

granular cytoplasm due to small lysosomes

precursor of macrophases and osteoclasts

  1. Neutrophil
44
Q

ID

A

Skeletal Muscle 400x

can see evidence of cross banding

Darker = a band

inbetween I bands

can see peripheral nuclei

45
Q

ID

A

Skeletal Muscle 1000x

thin preparation

little more detail

oil immersion

bands more clearly

Dark = A

Lighter = I

46
Q

ID

A

Skeletal Muscle 400x

harder to make out cross banding but still there

47
Q

ID

A

Skeletal Muscle x.s. 400x

cross-section

each block = Myofiber

Dark = nuclei at the periphery

48
Q

ID

A

NAD Stain (oxidative phosphorylation)

Type 1 Fibers = darker stain

Type II fibers stain lighter

the darker stain indicates high oxidative components

49
Q

ID

A

Smooth Muscle 400x

see fairly prominent middle nuclei

thin elongated cells

see some longitudinal banding and some plane not quite cross-section areas

50
Q

ID

A

Smooth Muscle (1000X)

see long tapering form of individual cells

51
Q

ID

A

Smooth Muscle, Teased (400x)

52
Q

ID

A

Cardiac Muscle 100x

not a lot of detail

even though striated not apparent

have striation of dark and light are intercalated dics

53
Q

ID

A

Cardiac Muscle 400x

impression of sarcomeric arrangement

intercalated

54
Q

ID

A

Cardiac muscle, Acute MI (400x)

intercalated dics

sarcomeric arrangement gone

some cells in process of death than others

can tell not normal

55
Q

ID

A

Skeletal Muscle : Gross Characteristics

  1. Fascicle
  2. myofiber

3, myofibril

  1. endomysium

5, epimysium

  1. blood vessels
  2. perimysium
56
Q

ID

A

Satellite cells

arrows: Myocyte Nuclei
arrowhead: Satellite cells

reserve “stem” cells

CD56 stains satellite cell perinuclear regions but does not stain differentiated myocytes

57
Q

ID

A

Muscle Spindles

Inside = intrafusal fibers

Dark with are the nuclei within the fibers

surrounding circle = muscle spindle

58
Q

ID

A

Muscle Spindles

Inside = intrafusal fibers

Dark with are the nuclei within the fibers

surrounding circle = muscle spindle

59
Q

ID

A

Cardiac Muscle: Purkinje Fibers 100x

actually modified cardiomyocytes

no sarcomere arrangement

very different but actually still derived

60
Q

ID

A

Nerve Bundle (x.s.; Trichrome)

  1. Perineurium
  2. Endoneurium
  3. Blood vessels
61
Q

ID

A

Microarchitecture of nerves

Silver Stain

  1. Myelin
  2. Axin
  3. Endoneurium
62
Q

ID

A
  1. Peripheral Nerve
  2. Epineurium
  3. Perineurium
  4. Fascicle
  5. Peripheral (myelinated) nerve fiber
  6. Endoneurium

7, Blood vessels supplying nerve (vasa nervorum)

  1. Myelin sheath (formed by neurolemma or Schwann cells)
  2. Axon
  3. Epineurium
  4. Perineurium
63
Q

ID

A

Purkinje Cell in Cerebellar Cortex

64
Q

ID

A

Cerebral cortex: Pyrimidal Cells

  1. Cell Body
  2. Dendrite
  3. Axon

all the area between often called the neural pile = lots of little bits of neurons and axons throughout from other cells

65
Q

ID

A

Golgi Type II neurons

66
Q

ID

A

Golgi Type I neuron types

67
Q

ID

A

Parts of a Neuron (myelinated axon)

  1. Schwann Cell
  2. Telodendrite
  3. Node of Ranvier
  4. Bouton
68
Q

ID

A

Parts of a Neuron

  1. cell body
  2. Myelinated Axon
  3. Dendrites
  4. Presynaptic Membranes with Vesicles
  5. telodendria and boutons
  6. postsynaptic membrane and receptors
69
Q

ID

A

Motor Neuron

  1. Glial Cell Nuclei
  2. Axon
  3. Axon Hillock
  4. Dendrites
70
Q

ID

A

Motor Neuron

  1. Dendrites
  2. Cell body
  3. supporting cells / Glial Cells
  4. Axon
71
Q

ID

A

Spinal cord 10mm embryo cross-section

  1. Mantle Layer
  2. Ependyma
  3. Lumen
  4. Marginal Layer

Sensory ganglia are the darker circles on the lower outside sides

72
Q

ID

A

Spinal Cord 10mm embryo cross-section

  1. Mantle Layer
  2. Ependyma
  3. Marginal Layer
  4. Lumen
73
Q

ID

A

Astrocytes around capillary

74
Q

ID

A

Astrocyte

75
Q

ID

A

Cornea, 100x

  1. Stroma

(can see arrayed in fairly organized manner collagen fibers

  1. Bowman’s Layer
  2. Corneal Epithelium
  3. Corneal Endothelium
  4. Descemet’s Membrane
76
Q

ID

A

Eye Dev. In 72 Hr Chick Embryo 100x

  1. Future Cornea
  2. Lens Vescicle
  3. Sensory Retina
  4. Pigmented Retina
77
Q

ID

A

Eye Dev in 6mm Pig Embryo 40x

  1. Diencephalon
  2. Optic Vesicle
  3. Skin Ectoderm
  4. Optic Stalk

(equivalent to 3 week human)

78
Q

ID

A

Retina 100x H& E stain

  1. Choroid
  2. Pigmented Epithelium
  3. Inner and Outer segments of rods and cones
  4. Outer Nuclear Layer
  5. Outer Plexiform Layer
  6. Inner Nuclear Layer (large nuclei that stain dark)
  7. Inner Plexiform Layer (refers to the dendrites and axons)
  8. Ganglion Cell Layer
  9. Axons of Ganglion cells

10 Internal Limiting Membrane

79
Q

ID

A

Retina 40x

H&E stain

  1. Sclera

2, Choroid

  1. Retina
80
Q

ID

A

Eyelid 40x

  1. Tarsal Glands
  2. Orbicularis

Black outer edge all Epithelium; statified squamous

81
Q

ID

A

Cochlea xs. 100x

  1. Scala vestibuli w/ perilymph
  2. Vestibular membrane
  3. Internal spiral tunnel of cochlear duct
  4. Tectorial Membrane
  5. Inner hair cells (don’t see but are embedded)
  6. inner tunnel
  7. Cochlear duct
  8. Stria vascularis
  9. Outer hair cells
  10. Basilar membrane
  11. Scala tympani w/ Perilymph
82
Q

ID

A

Cochlea xs. 40x

  1. Scala Vestibuli
  2. Vestibular membrane
  3. Cochlear Duct
  4. Stria vascularis
  5. Orgn of Corti
  6. Basilar membrane
  7. Scala Tympani
  8. cell bodies of the spinal ganglia
83
Q

ID

A
  1. Malleus
  2. Incus
  3. Stapes
  4. Scala tympani
  5. Scala vestibuli
  6. Cochlear Nerve
  7. Spiral ganglion
  8. Cochlea
  9. Round window
  10. Oval Window
  11. Tympanic membrane
  12. Auditory Canal
84
Q

ID

A

External Ear LS. 100x

  1. Blood vessel
  2. Elastic Cartilage
85
Q

ID

A

External Ear LS. 100x

  1. Integument
  2. Hair Follicle
  3. Blood Vessel
86
Q

ID

A

External Ear LS> 100x

  1. Integument (stratified squamous)
  2. Hair Follicle
  3. Elastic Cartilage
  4. Blood Vessels (light pink = packed RBC’s)
87
Q

ID

A

Promegakaryocyte

88
Q

ID

A

Promegakaryocyte

89
Q

ID

A

Proerythroblast

90
Q

ID

A

Proerythroblast

91
Q

ID

A

Reticulocytes

92
Q

ID

A

Pseudounipolar neuron in dorsal root ganglion

Histology - Katie (4 decks)

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