exam 2 bio Flashcards
ch 6-10
Membrane phospholipids?
may have ʺkinksʺ in their tails, each caused by the presence of a double bond between carbons.
One difference between eukaryotic and prokaryotic cells is that eukaryotic cells
have membrane-enclosed organelles, which are lacking in prokaryotic cells.
Which of the following options best depicts the flow of information when a gene directs the synthesis of a cellular component?
DNA —> RNA —–>protein
Which of the following choices correctly matches a tool and its proper application?
cell fractionation to study the function of specific organelles
Which of the following microscopy techniques is best suited for visualizing protein complexes in their cellular environments without the use of preservatives?
Cryo-electron microscopy
Which of the following clues would tell you if a cell is prokaryotic or eukaryotic?
whether or not the cell is generally partitioned by internal membranes
Which of the following correctly matches an organelle with its function?
central vacuole with storage
Which structure is common to plant and animal cells?
Mitochondrion
Which of the following statements best describes one major difference between prokaryotic cells and eukaryotic cells?
Eukaryotic cells have membrane-bound organelles, while prokaryotic cells do not.
Which of the following structures is common to plant and animal cells?
mitochondrion
Which of the following areas in bacterial cells best describes where chromosomal DNA is generally localized?
In the nucleoid
Which of the following parameters is most likely to limit the maximum size of a cell?
The ratio of surface area to volume of cytoplasm
Select the correct statement describing cellular structure or function.
Plant and animal cells both carry out cellular respiration, producing ATP.
Beginning within the nucleus, the first step leading to the synthesis of a polypeptide is _____.
Transferring of information from DNA to messenger RNA
Which molecules do not normally cross the nuclear membrane?
DNA
Which of the following statements about the nuclear envelope is false?
The nuclear envelope is continuous with the Golgi apparatus.
True or false? Large proteins containing a nuclear localization signal (NLS) bind to the nuclear pore and enter the nucleus without any expenditure of energy.
False
A small protein (molecular weight = 25,000 daltons) is injected into a cell and observed in the nucleus a short time later. What type of transport has taken place?
Passive transport
In experiments to test whether a protein can enter the nucleus, why would proteins be labeled with fluorescent molecules?
To make the proteins easy to see
Nucleoplasmin is a nuclear protein. This protein was divided into two segments and linked to the same large cytoplasmic protein, generating two fusion proteins. After injecting these fusion proteins into a cell, one of the proteins was found in the nucleus and the other in the cytoplasm. Which of the following conclusions can be drawn from these results?
Only one of the two fusion proteins possesses a nuclear localization signal.
SCIENTIFIC INQUIRY
Imagine protein X, destined to span the plasma membrane; assume the mRNA carrying the genetic message for protein X has been translated by ribosomes in a cell culture. If you fractionate the cells, in which fraction would you find protein X?
Pellet rich in “microsomes”
Which of the following statements best describes the function of the nuclear pore complex found in eukaryotic cells?
It regulates the movement of proteins and RNAs into and out of the nucleus.
Disruption of nuclear pore complexes in a eukaryotic cell will most likely interfere with the transport of which of the following molecules out of the nucleus?
mRNA
Which organelle plays a role in intracellular digestion?
lysosome