Exam 2

Question 1

Regulation of citric acid cycle

Answer 1

Pyruvate dehydrogenase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase

Question 2

What inhibits PDH?

Answer 2

ATP, Acetyl co A, NADH

Question 3

What inhibits isocitrate dehydrogenase?

Answer 3

ATP, NADH

Question 4

What inhibits alpha ketoglutarate dehydrogenase?

Answer 4

ATP, succinyl coa, NADH

Question 5

Defense mechanisms against ros?

Answer 5

Superoxide dismutase and catalase

Question 6

Example of chemical uncoupler

Answer 6

UCP-1

Question 7

How do uncouplers work?

Answer 7

Disrupt proton gradient

Question 8

Inhibitors of oxidative phosphorylation

Answer 8

Cyanide, carbon monoxide, azine, antimycin A, rotenone, amytal

Question 9

Rotenone and Amytal

Answer 9

Block electron transfer in nadh-q oxidoreductase, prevent NADH as substrate

Question 10

Antimycin A

Answer 10

Interferes with electron flow in q-cytochrome c oxidoreductase

Question 11

Carbon monoxide,azide, cyanide

Answer 11

Cyanide and azide react with iron + 3, Carbon monoxide with iron +2. Prevent transfer to oxygen

Question 12

Number of photons to products

Answer 12

8 photons →1 oxygen molecule,2NADPH, 3 ATP

Question 13

Net reaction of citric acid cycle

Answer 13

AcetylCoA +3 NAD+ + FAD + ADP + p+2 water →2 carbon dioxide +3NADH+FADH2+ ATP +2H++ co A

Question 14

Net reaction of etc

Answer 14

NADH + 1/2 oxygen + H+ → water +NAD+

Question 15

DNP

Answer 15

Drug that works as a mild uncoupler

Question 16

Excitation/resonance transfer

Answer 16

Excitement energy transferred to nearby molecules

Question 17

Photooxidation

Answer 17

Charge separation from electron transfer

Question 18

Order of proteins in photosynthesis

Answer 18

Photo-system 2, plastoquinone, b6F complex, plastocyanin, photosystem 1, ferredoxin, NADp reductase

Question 19

Fates of an electronically excited molecule

Answer 19

Internal conversion, fluorescence, excitation transfer, photooxidation

Question 20

Internal conversion

Answer 20

Loss as heat

Question 21

Fluorescence

Answer 21

Loss as light

Question 22

Net reaction of photosynthesis

Answer 22

2 water +8 photons + 10 H+ (stroma) + 2 NADP +→ oxygen molecule +2 NADPH + 12 H+ (in lumen)

Question 23

ATP synthesis

Answer 23

8 photons required to generate 3 molecules of ATP

Question 24

Net reaction of ATP synthesis in photosynthesis

Answer 24

2 NADP+ + 3adp +3p +H (stroma) → oxygen molecule +2 NADPH +3 ATP + water

Question 25

Cyclic phosphorylation

Answer 25

Produces ATP instead of NADPH

Only in PS 1