Exam 2 Flashcards
ID the organism and stage.

Chilomastix mesnili
Trophozoite
ID the organism and stage.

Chilomastix mesnili
Cyst
ID the organism and stage.

Chilomastix mesnili
Cyst
ID the organism and stage.

Trypanosomas Brucei gambiense (trypomastigote)
or
Trypanosomas Brucei rhodiesense (trypomastigote)
*we don’t need to distinguish between the 2 species specifically
ID the organism and stage.
Give 2 features.

Trypanosomas cruzi (trypomastigote)
- Prominent C-shaped body
- Note the large, posterior kinetoplast
*the kinetoplast can potentially be smaller as well
ID the organism and stage.

Trypanosomas Brucei gambiense
or
Trypanosomas Brucei rhodiesense
*we don’t need to distinguish between the 2 species specifically
- What stage is shown?
- (if possible give an example of what it may be)
- Give 2 defining features

-
Amastigote
- L. Donovani or T. cruzi; mostly indistinguishable
- Features:
- rounder shape
- short, or non-apparent flagella
What organism could be shown?
ID the stage of organism shown.
Extra credit: Acute or Chronic?

- T. Cruzi* or L. Donovani*
- Amastigote [pseudocyst]
- Chronic
*they are indistinguishable
ID the organism and stage.
Give 2 characteristics.

- Leishmania Donovani* (promastigote)
- Note the absence of an undulating membrane, and the anterior location of the kinetoplast relative to the nucleus
ID the organism and stage.
Give 2 features.

- Leishmania Donovani* (promastigote)
- Note the absence of an undulating membrane, and the anterior location of the kinetoplast relative to the nucleus
ID the organism and stage.

Leishmani tropica (promastigote)
ID the organism and stage.

Giardia Lamblia
Trophozoite
ID the organism and stage.

Giardia lamblia
Cyst
ID the organism and stage.

Trichomonas hominis
ID the organism and stage.

Trichomonas Vaginalis
Trophozoite
For life cycle’s completion, the fly must ingest in its blood meal the ____________, which is physiologically adapted for existence within the insect vector.
short, stumpy trypomastigote
The short stumpy trypomastigote has ______ that allow it to survive effectively in an insect host.
Prominent cristae
In mosquitos, which sexes feed on blood?
Female only
In Tsetse flys, which sexes feed on blood?
Both sexes
What is the ultimate location in a human that Trypanosomas Brucei metacyclic trypomastigotes reach?
CSF
In blood, what are the 3 stages that T. brucei may take in blood?
- long slender
- intermediate
- short, stumpy
What organism is shown?

Trypanosoma
ID the organism and stage.
What key factor(s) do you see?

- Babesia* trophozoites
- tetrads noted [cross formations]
ID the organism and stage.
What key factor(s) do you see?

Babesia trophozoite
tetrads [cross formations]
ID the organism and stage.

- Cryptosporidum Parvum*
- Oocyst
ID the organism and stage.

Cryptosporidium Oocyst
ID the organism and stage.

- Cryptosporidium Parvum*
- Oocyst
ID the organism.
What is at the yellow label?
What is at the green label?
Give defining characteristics.

Plasmodium vivax
- yellow = schizont
- 16-24 merozoites
- enlarged RBC
- green = trophozoite
- more amoeboid shape
ID the organism.
What is seen at the arrow?

- Cryptospiridium parvum*
- Oocyst
ID the organism at the center right.
What stage is it in?
Give characteristics.

Plasmodium Vivax
- gametocyte
- enlarged RBC; round/oval
ID the organism.
What stage is shown?
What characteristics to ID?

P. falciparum
Gametocyte
- banana-shaped
*on the exam
ID the organism.
What stage is shown?
What characteristics did you use?

P. falciparum
- Signet ring stage
- Same size RBC
ID the organsim.
What stage is shown?
What characteristic to ID?

P. falciparum
- Schizont
- 8-32 merozoites in schizont
ID the stage/structure shown.

Oocyst
ID the organism.
What stage is it in?
What key structure do you see?

P. ovale
- Schizont*
- enlarged cells; large schuffer’s dots; 4-16 merozoites
*not confirmed
What organism do you see?
What stage is it in?
What characteristic gave it away?

P. malarie
- Trophozoite
- Band stage full of ziemann dots
ID the organism and stage shown.

- Taxoplasma Gandii*
- tachyzoites
ID the organism and stage shown.
What unique structure is shown?

Taxoplasma Gandii
- bradyzoites
- pseudocyst
[not confirmed; while the individual shapes appear more elongate (like a tachyzoite), this specimen is in tissue]
Which organism and stage is shown?

- Taxoplasma gandii*
- Bradyzoites
Which organism and stage is shown?

- T. gondii*
- Unsporulated Oocyst
ID the organism and stage shown.
What is seen at the arrows?

T. gandii
- Sporulated Oocyst
- Four sporozoites (arrows) are visible in one of the sporocysts
ID the organism and stage.

- T. gandii*
- sporulated oocyst
ID the species shown in each picture:
- top left =
- top right =
- bottom left =
- bottom right =

- Top left: Plasmodium falciiparum
- Top Right: Plasmodium vivax
- Bottom Left: Plasmodium malariae
- Bottom right: Plasmodium ovale
[baduri said dont worry about the dots too much]
WTF is this?
What typa insect is it?
What parasite does it carry?

Anopholes (mouth parts)
- Mosquito
- Plasmodium
WTF is this?
What typa insect is it?
What parasite does it carry?

Ixodes scapularis
- Tick
- Bebesia microti and divergens
ID the organism and stage.
Which characteristics did you use?

P. Vivax
- signet stage
- enlarged RBC
- more amoeboid shape
ID the organism and stage.

- P. Vivax*
- ovular gametocyte
ID the organism and stage.
Which characteristics did you use?

P. malarie
- Signet ring stage
- the cell is normal/small size; ring is stretched a lil
ID the organism and stage.
Which characteristics did you use?

P. Ovale
- signet ring stage
- enlarged RBC, slight oval shape
ID the organism and stage.
Which characteristics did you use?

P. Ovale
- Schizont
- slightly enlarged RBC; oval-shaped; tufted edges; schuffer’s dots
ID the organism and stage.
Which characteristics did you use?

P. Ovale
- schizont
- slightly enlarged RBC; oval-shaped; tufted edges; schuffer’s dots
For P. Vivax and P. Ovale:
_______________ are distributed throughout RBC’s cytoplasm & stains pink to red when subjected to traditional hematological stains
schuffner’s dots
Coarser, dark _________ granules, the by-products of hemoglobin degradation by the parasite
hemozoin
For P. malariae:
Following entry into RBC, early trophozoites begin to accumulate hemozoin and the pink-stained _________ ________.
Ziemann’s dots
For each picture, state what structures are seen in the infected RBC.
Give an example of which plasmodium caused it.

-
Schuffner’s dots
- P. Vivax /P. Ovale
-
Ziemann’s dots
- P. Malarie
-
Hemozoin granules
- P. Vivax / P. Ovale / P. Malarie
-
Maurer’s dots
- P. Falciparum
[reference Apicomplexans powerpoints]
Which plasmodium is most prevalent?
P. Falciparum
80% of cases in Africa
How do the life cycles of Plasmodium and Babesia differ?
In Bebisia:
- no exoerythrocytic stage
- no sexual phase in the vertebrate portion of the life cycle.
What are the 2 types of infective stages of Toxoplasma gandii?
- Oocyst [fecal material from cat]
- Tissue cyst [undercooked meat from pig, sheep]
For Toxoplasma Gandii:
- All animals except ____ can be considered as paratenic hosts
- Formation of pseudocysts coincides with development of _______ in the host
cats
immunity
for the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents wtih c/o fatty stool, weight loss, ABD pain and jaundice.
- Giardia Lamblia
- Giardiasis
- Metronidazole
For the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with c/o vaginal itching and odor + leukorrhea.
- Trichomonas Vaginalis
- Vaginitis [trichomoniasis]
- Metronidazole, Acidic douches and abstinence
For the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with c/o weakness, anemia and protruding abdomen.
- L. Donovani
- Kala Azar [visceral leishmaniasis]
- Antimony
For the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with c/o fever, malaise, facial lesions with facial scarring.

- L. Donovani
- Post-kala-azar dermal leishmanoid
- Antimony
for the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with c/o of a dry lesion (cutaneous ulcer) on the right finger, it has persisted for months. He lives in Mexico and also visits Brazil.
Lab testing reveals many mastigotes of a certain species.

- L. Tropica [Phlebotomus sandly]
- Cutaneous Leishmaniasis
- Antimony**
**this isn’t confirmed, but is used for L. Donovani and L. Braziliensis
for the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with c/o moist skin ulcerations with a quick onset.
Lab studies show, few mastigotes of a species.

- L. Major [Phlebotomus sandfly]
- Cutaneous Leishmaniasis
- Antimony**
**not confirmed, but used for L. Donovani and L. Braziliensis
Pt presents with a many small, red ulcers full of amastigotes.
He lives in Central America, no recent travels.

- L. Braziliensis [Lutzomyia sandfly]
- Muco-cutaneous leishmaniasis or American Leishmaniasis
- Antimony
For the given history, provide: Parasite, relevant disease names and Treatment:
Pt presents with complaints of a fever, cough, breathing difficulty and cyanosis.
Pneumocystis Carinii
- Asphyxia
- TMP-SMA