Exam 2 Flashcards
which genera of parasites can be detected and identified by examination of a peripheral blood smear?
plasmodium
babesia
leishmania
what are the 4 components of the procedure for the laboratory examination of a stool specimen for ova and parasites?
macroscopic exam
microscopic exam
concentration
permanent stain smear procedure
name the 2 components commonly used for the concentration of a stool specimen and give the principle of each procedure
formalin ether/ethyl acetate - sedementation
zinc sulfate - floatation
why is it useful to perform the direct examination of a stool specimen with saline and iodine?
saline is to view anything motile
iodine is to observe cyst of protozoa (stains nuclei)
name several artifacts that can be found in ova and parasite preps
pollen grains WBC RBC yeast plant fibers
what is a scotch tape preperation and for what organism is it used?
enterobius vermicularis (pinworm) sticky surface to the anal opening at bedtime. Used to collect eggs from perianal area.
how soon after collection should a semi-solid or liquid stool be examined?
semi solid or liquid within 30 mins
how soon after collection should a formed stool be examined?
macro or micro must be done same day, time permitting.
if formed stool cannot be examined on the day of collection, what steps should be taken?
put speicmen in a preserative within 30 mins and refrigerate
what fixative should be used with a liquid stool?
liquid PVA (poly vinyl alcohol)
what fixative should be used with a formed stool?
10% formalin
what type of container is best to use for the collection and transporting of a stool specimen?
clean dry container (does not have to be sterile)
name several speicmens other than stools that might be examined for ova and parasites
blood urine perianal swab sputum duodenal
what are charcot-leyden crystals?
artifacts found in stool that indicated inflammation
what is the significance of charcot-leyden crystals?
parasite infection or allergic reaction
