Exam 2 Flashcards

1
Q

Ti Plasmid

A

bacterial genetic material that is transferred that resides on an extra-chromosomal element in the bacteria

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2
Q

T-DNA

A

portion of the Ti plasmid transferred to the plant

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3
Q

promoter

A

upstsream control sequence

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4
Q

auxins and cytokinins

A

plant hormones

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5
Q

opines

A

food source for agrobacterium cells living in plant system

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6
Q

primary structure

A

amino acid sequence of protein

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7
Q

acid phosphattase

A

enzyme

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8
Q

rate of reaction

A

substrate converted (moles)/time

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9
Q

p-nitrophenol

A

product

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10
Q

activation energy

A

amount of energy that must be imparted to the reacting molecules in order to permit them to react

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11
Q

Arrhenius equation

A

log (v2/v1) = (Ea/4.6) (1/T1-!/T2)

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12
Q

van’t Hoff equation

A

log Q10 = (10/[T2-T1]) (log[v2/v1])

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13
Q

Q10

A

temperature coefficient- how much reaction rate goes up per 10 degree increase in temperaturre

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14
Q

Vmax

A

rate at saturation

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15
Q

Km

A

substrate concentration at 1/2 Vmax

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16
Q

competitive inhibitor

A

bind reversibly to enzyme and prevent it from binding to substrate

17
Q

do competitive inhibitors have impact on Vmax?

A

no

18
Q

do competitive inhibitors have impact on Km?

A

yes, they raise it.

19
Q

un-competitive inhibiror

A

reversibly bind to enzyme:substrate complex but not to the free enzyme

20
Q

do un-competitive inhibitors have impact on Vmax?

A

yes, they reduce it

21
Q

do un-competitive inhibitors have impact on Km?

A

yes, they reduce it