Exam 2 Flashcards
Ti Plasmid
bacterial genetic material that is transferred that resides on an extra-chromosomal element in the bacteria
T-DNA
portion of the Ti plasmid transferred to the plant
promoter
upstsream control sequence
auxins and cytokinins
plant hormones
opines
food source for agrobacterium cells living in plant system
primary structure
amino acid sequence of protein
acid phosphattase
enzyme
rate of reaction
substrate converted (moles)/time
p-nitrophenol
product
activation energy
amount of energy that must be imparted to the reacting molecules in order to permit them to react
Arrhenius equation
log (v2/v1) = (Ea/4.6) (1/T1-!/T2)
van’t Hoff equation
log Q10 = (10/[T2-T1]) (log[v2/v1])
Q10
temperature coefficient- how much reaction rate goes up per 10 degree increase in temperaturre
Vmax
rate at saturation
Km
substrate concentration at 1/2 Vmax
competitive inhibitor
bind reversibly to enzyme and prevent it from binding to substrate
do competitive inhibitors have impact on Vmax?
no
do competitive inhibitors have impact on Km?
yes, they raise it.
un-competitive inhibiror
reversibly bind to enzyme:substrate complex but not to the free enzyme
do un-competitive inhibitors have impact on Vmax?
yes, they reduce it
do un-competitive inhibitors have impact on Km?
yes, they reduce it
