Exam 2

Question 1

DNAa

Answer 1

Binds to origin of replication (DNA Template Prep 1)

Question 2

Helicase

Answer 2

Unwinds double helix, attaches the single stranded DNA and continues to separate (DNA Template Prep 2)

Question 3

Single-stranded binding proteins

Answer 3

Binds to single strained DNA to keep it from rennealing, keep helix open (DNA template prep 3)

Question 4

Topisomerase

Answer 4

Relives super-coming that builds up, relaxes supercoiled DNA by breaking one or both strands, allowing DNA to rotate then rejoining the strands (DNA template prep 4)

Question 5

Primase

Answer 5

Synthesizes short RNA primers, creates RNA primers to initiate synthesis (DNA synthesis 1)

Question 6

DNA polymerase III

Answer 6

Stendhal off the primer until it runs into another primer, produces new strands of complementary DNA (DNA synthesis 2)

Question 7

DNA polymerase I

Answer 7

Extends off the DNA synthesized by another primer, fills in gaps between newly synthesized Okazaki segments (DNA synthesis 3)

Question 8

DNA Ligase

Answer 8

Connects adjacent fragments once RNA is replaced, welds together Okazaki fragments (DNA synthesis 4)