EXAM '11,'10,'09

Question 1

A mutation is S.pombe that results in longer G2 than normal is predicted to result in:

Answer 1

bigger cells

Question 2

Mouse ES cells are said to be totipotent. This means they:

Answer 2

are able to form any of the cell types of the adult mouse

Question 3

A constitutively-expressed gene is one which is:

Answer 3

expressed at all times

Question 4

A 2 component signal transduction mechanism in E.coli involves a sensor

Answer 4

with kinase activity

Question 5

In a DNA modification protection/footprinting experiment

Answer 5

1. protein is bound to labelled DNA
2. treated with DNAseI
3. DENATURED
4. ELECTROPHORESED

Question 6

Trans-splicing

Answer 6

attaches a common exon sequence to a variety of mRNA molecules encoded by different genes

Question 7

Alternative splicing:

Answer 7

mRNAs containing different assortments of exons are generated from a single gene

Question 8

Intein

Answer 8

Polypeptide sequence that is removed from precursor polypeptide

Question 9

CYCLINS

Answer 9

Proteins that are synthesized and degraded at specific times within each cell cycle and regulate protein kinases

Question 10

Centromeric (YCp) plasmids in S. cerevisiae are

Answer 10

replicated as small circular plasmids in a single copy