EXAM 1 REVIEW Flashcards

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1
Q

specialized area of biology that deals with living things ordinarily too small to be seen without magnification

A

microbiology

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2
Q

invisible to the naked eye

A

microscopic

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3
Q

a living thing ordinarily too small to be seen without magnification; an organism of microscopic size

A

microorganisms

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4
Q

microorganism

A

microbes

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5
Q

category of prokaryotes w/ peptidoglycan in their cell walls and circular chromosome(s). This group of small cells is widely distributed in the earth’s habitats.

A

bacteria

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6
Q

microscopic, acellular agent composed of nucleic acid surrounded by a protein coat

A

viruses

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7
Q

a group of single-celled, eukaryotic organisms

A

protozoa

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8
Q

term that designates all parasitic worms

A

helminths

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9
Q

macroscopic and microscopic heterotrophic eukaryotic organisms that can be uni- or multicellular

A

fungi

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10
Q

a member of the domain Eukarya whose cells have a well-defined nucleus and membrane-bound organelles; includes plants, animals, fungi, protozoa, and algae

A

eukaryote

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11
Q

a single-celled organism that does not have special structures such as a nucleus or membrane-bound organelles; includes bacteria and archaea

A

prokaryote

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12
Q

accumulation of changes that occur in organisms as they adapt to their environments

A

evolution

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13
Q

evidence cited to explain how evolution occurs

A

theory of evolution

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14
Q

process occurring in plants, algae, and some bacteria that traps the sun’s energy and converts it to ATP in the cell. This energy is used to fix CO2 into organic compounds.

A

photosynthesis

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15
Q

breakdown of dead matter and wastes into simple compounds that can be directed back into the natural cycle of living things

A

decomposition

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16
Q

field involving deliberate alteration (recombination) of the genomes of microbes, plants , and animals though special technological processes

A

genetic engineering

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17
Q

technology aka genetic engineering; deliberately modifies the genetic structure of an organism to create novel products, microbes, animals, plants and viruses

A

recombinant DNA technology

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18
Q

decomposition of harmful chemicals by microbes or consortia of microbes

A

bioremediation

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19
Q

any agent that causes diseases; usually a virus, bacterium, fungus, protozoan, or helminth

A

pathogens

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20
Q

prokaryotic single celled organisms of primitive origin that have unusual anatomy, physiology, and genetics and live in harsh habitats

A

archaea

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21
Q

one of the three domains of living organisms, as proposed by Woese; contains all eukaryotic organisms

A

eukarya

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22
Q

an elementary virus particle in its complete morphological and thus infectious form; consists of nucleic acid core surrounded by a capsid, which can be enclosed in an envelope

A

virion

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23
Q

an organism that lives on or within another organism (the host), from which it obtains nutrients and enjoys protection; produces some degree of harm in the host

A

parasites

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24
Q

small component of eukaryotic cells that is bounded by a membrane and specialized in function

A

organelles

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25
Q

organism in which smaller organisms or viruses live, feed and reproduce

A

hosts

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26
Q

early belief that living things arose from vital forces present in nonliving or decomposing matter

A

spontaneous generation

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27
Q

belief in spontaneous generation as a source of life

A

abiogenesis

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28
Q

a complex association that arises from a mixture of microorganisms growing together on the surface of a habitat

A

biofilms

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29
Q

a techniques that amplifies segments of DNA for testing.; using denaturation, primers, and heat-resistant DNA polymerase, the number can be increased several-million-fold

A

polymerase chain reaction (PCR)

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30
Q

principles and procedures for the systematic pursuit of knowledge, involving the recognition and formulation of a problem, the collection of data through observation and experimentation and the formation and testing of a hypothesis.

A

scientific method

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31
Q

tentative explanation of what has been observed or measured

A

hypothesis

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32
Q

collection of statements, propositions, or concepts, that explains or accounts for a natural event

A

theory

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33
Q

completely free from all life forms, including spores and viruses

A

sterile

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34
Q

method of handling microbial cultures, patient specimens, and other sources of microbes in a way that prevents infection of the handler and other who may be exposed

A

aseptic techniques

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35
Q

originated in 1800s that proposed that microorganisms can be the cause of diseases; established in the present time that is tis considered a fact

A

germ theory of disease

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36
Q

a set system for scientifically naming organisms, enzymes, anatomical structures, and so on

A

nomenclature

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37
Q

formal system for organizing, classifying, and naming living things

A

taxonomy

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38
Q

taxonomic categories

A

taxa

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39
Q

scientific method of assigning names to organisms that employs two names to identify every organism- genus name plus species name

A

binomial system

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40
Q

the broadest general category to which an organism is assigned member share only one or a few general characteristics

A

domain

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41
Q

second division of classification

A

kingdom

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42
Q

third level of classification

A

phylum

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43
Q

alternate term for phylum

A

division

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44
Q

division of organisms that follows phylum

A

class

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45
Q

division of organisms that follows class; increasing similarity may be noticed among organisms assigned to the same order

A

order

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46
Q

midlevel division of organisms that groups more closely related organism than previous levels

A

family

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47
Q

second most specific level

A

genus

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48
Q

most specific level of organization

A

species

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49
Q

levels of power; arrangement in order of rank

A

hierarchies

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50
Q

bacteria, viruses, fungi, algae, protozoa, helminths

A

major microbe groups

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51
Q

When did bacteria, archaea, eukaryotes arise?

A

2.9 billion years ago

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52
Q

What important roles do microbes play on earth for life to exist?

A

play centrals roles essential to life-nutrient cycling, decomposition, maintain atmosphere and regulate temperature

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53
Q

What is the rank of infectious disease death in the US for: lower respiratory flu/pneumonia

A

lower respiratory: #3 (141,00) flu/pneumonia: #8 (56,000

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54
Q

What is the rank of infectious disease death worldwide for lower respiratory from flu/pneumonia, diarrheal disease, HIV/AIDS and TB (in order)?

A

3: lower respiratory from flu/pneumonia (3.5 million) #5: diarrheal disease (2.5 million) #6: HIV/AIDS (1.8 million) #8: TB (1.3 million)

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55
Q

What is pneumonia and what can cause it?

A

inflammation of alveoli, fluid can be caused by influenza, a different virus or bacteria

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56
Q

Why is the flu more severe this season?

A

H3N2 mutates faster more difficult to grow H3N2 in eggs in the process of adapting virus to grow in eggs, introduce further changes to the virus, which may impair effectiveness of vaccine flu shots 30% effective

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57
Q

What is the most infectious microbe (non-human transmission)?

A

malaria

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58
Q

What is the most infectious microbe (human to human transmission)?

A

measles

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59
Q

What are some examples of emerging diseases?

A

SARS MERS Lassa fever Ebola HIV Nipah virus

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60
Q

What are some examples of re-emerging diseases?

A

yellow fever cholera plague dengue whooping cough (pertussis) West Nile measles

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61
Q

What are some examples of diseases considered noninfectious, now found to be caused by microbes?

A

gastric ulcers: caused by Helicobacter pylori link between cancers and bacteria and viruses Cocksackie virus associated with diabetes Borna agent linked to schrizophenia

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62
Q

About 10X smaller than eukaryotic cells lack organelles single celled

A

bacterial and Archaeal cells

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63
Q

Consist of ribosomes, chromosomes, cell wall, cell membrane and flagellum

A

Bacteria and Archaea

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64
Q

Consists of ribosomes, nucleus, mitochondria, cell membrane, flagellum

A

Eukaryotes

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65
Q

Consist of envelope, Capsid, nucleic acid

A

Virus

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66
Q

Is a virus considered a cell?

A

No

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67
Q

What is an example of a helminth?

A

Taenia solium (looks like a worm with a head)

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68
Q

What is an example of a fungus?

A

Syncephalastrum (looks like a flower)

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69
Q

What is an example of protozoan?

A

Vorticella (looks like little vortex of a tornado)

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70
Q

What is an example of a bacterium?

A

E. coli (looks like little tiny eggs like lice)

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71
Q

What is an example of a virus?

A

Herpes simplex (looks like craters)

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72
Q

Where are the majority of microorganisms found and are they harmful?

A

habitats such as soil and water either harmless or beneficial

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73
Q

What type of microbe is harbored and nourished by the host; causes damage and disease in the host; and some have symbiotic relationships?

A

Parasites

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74
Q

Who performed the experiment disproving spontaneous generation?

A

Francesco Redi

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75
Q

What experiments disproved spontaneous generation?

A

Placed meat into two jars and covered one with fine gauze, preventing flies form lading on it leaving the other uncovered (Redi) Showed that the dust in in air were the source of growth in infusions and broth (Pasteur)

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76
Q

Who provided the earliest record of microbes by using a single-lens microscope made by himself?

A

Robert Hooke

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77
Q

Who made a crude microscope to examine threads in fabrics, made drawings of what he called “animalcules” in rainwater and scraped from his teeth; discovered first live cells?

A

Antonie van Leeuwenhoek

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78
Q

Who observed that mothers who gave birth at home experienced fewer infections than mothers who gave birth in the hospital?

A

Oliver Wendell Holmes

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79
Q

Who showed quite clearly that women became infected in the maternity ward after examinations by physicians coming directly from the autopsy room?

A

Ignaz Semmelweiz

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80
Q

Who was the first to introduce aseptic techniques such as hand washing and misting operating rooms with antiseptic chemicals to reduce microbe number?

A

Joseph Lister

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81
Q

Who invented pasteurization and completed some of the first studies showing that human diseases could arise from infection?

A

Louis Pasteur

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82
Q

Who linked a specific microorganism with a specific disease, established a set of proofs that verified the germ theory of disease, showed anthrax caused by Bacillus anthracis, invented inoculation, isolation, media, culture maintenance and specimen prep?

A

Robert Koch

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83
Q

What is it called when you make an observation and create a general principle?

A

inductive reasoning

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84
Q

What is called when you make an observation using an accepted general principle as a guide to explain specific observations?

A

deductive reasoning

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85
Q

Science is a process of _________?

A

observation, experimentation and reasoning

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86
Q

What are the levels of classification from most general to most specific? “Dear Katie Please Come Over For Great Snacks”

A

Domain Kingdom Phylum Class Order Family Genus Species

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87
Q

What is the proper way to write a scientific name?

A

Genus/Species name Genus name is always capitalized/can be abbreviated Species name always begin with lower case Both names should be italicized when in print or underlined when written

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88
Q

tangible materials that occupy space and have mass

A

matter

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89
Q

smallest particle of an element to retain all the properties of that element

A

atom

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90
Q

elementary particle that carries a positive charge, identical to the nucleus of they hydrogen atom

A

protons

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91
Q

electrically neutral particle in the nuclei of all atoms except hydrogen

A

neutrons

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92
Q

negative charged subatomic particle that is distributed around the nucleus in an atom

A

electrons

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93
Q

substance comprising only one kind of atom that cannot be degraded into two or more substances without losing its chemical characteristics

A

elements

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94
Q

version of an element that is virtually identical in all chemical properties to another version except that their atoms have slightly different atomic masses

A

isotopes

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95
Q

pathways of electrons as they rotate around the nucleus of an atom

A

orbitals

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96
Q

distinct chemical substance that results from the combination of two or more atoms

A

molecule

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97
Q

molecules that are combination of two or more different elements

A

compounds

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98
Q

link formed between molecules when tow or more atoms share, donate, or accept electrons

A

chemical bonds

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99
Q

combining power of an atom based upon the number of electrons it can either take on or give up

A

valence

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100
Q

chemical bond formed by the sharing of electrons between 2 atoms

A

covalent bonds

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101
Q

a molecule with asymmetrical distribution of charges, such a molecule has a positive and a negative pole

A

polar

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102
Q

electrically neutral molecule formed by covalent bonds between atoms that have the same or similar electronegativity

A

nonpolar

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103
Q

aqueous dissociation of an electrolyte into ions

A

ionization

104
Q

unattached, charged particle

A

ions

105
Q

positive charged ion

A

cations

106
Q

negative charged ion

A

anions

107
Q

any compound that ionizes in solution and conducts current in an electrical field

A

electrolytes

108
Q

weak chemical bond formed by attraction of forces between molecules or atoms in this case hydrogen and either oxygen or nitrogen; electrons are not shared , lost or gained.

A

hydrogen bond

109
Q

redox reactions in which paired sets of molecules participate in electron transfers

A

oxidation reduction

110
Q

denoting an oxidation-reduction reaction

A

redox

111
Q

weak attractive interactions between molecules of low polarity

A

van der Waals forces

112
Q

molecules entering or starting a chemical reaction

A

reactants

113
Q

substance that is left after a reaction is complete

A

products

114
Q

substance that alters the rate of a reaction without being consumed or permanently changed by it. Example: enzymes

A

catalysts

115
Q

mixture of one or more substances (solutes) that cannot be separated by filtration or ordinary settling

A

solution

116
Q

substance that is uniformly dispersed in a dissolving medium or solvent

A

solutes

117
Q

dissolving medium

A

solvent

118
Q

the addition of water as in the coating of ions with water molecules as ions enter into aqueous solution

A

hydrated

119
Q

property of attracting water

A

hydrophilic

120
Q

property of repelling water

A

hydrophobic

121
Q

expression of the amount of a solute dissolved in a certain amount of solvent; may be defined by weight, volume or percentage

A

concentration

122
Q

solution with a pH value below 7 on the pH scale

A

acidic

123
Q

solution with a pH value above 7 on the pH scale

A

basic

124
Q

system for rating acidity and alkalinity

A

pH

125
Q

process of combining an acid and a base until they reach a balanced proportion with a pH value close to 7

A

neutralization

126
Q

totality of chemical and physical processes occurring in a cell

A

metabolism

127
Q

molecules that lack the basic framework of the elements of carbon and hydrogen

A

inorganic chemicals

128
Q

molecules that contain the basic framework of the element of carbon and hydrogen

A

organic chemicals

129
Q

molecular combination that reacts in predictable ways and confers particular properties on a compound Example: —-COOH,—OH, —-CHO

A

functional groups

130
Q

study of organic compounds produced by living things Example: carbs, lipids, proteins, and nucleic acids

A

biochemistry

131
Q

large molecular compounds assembled from smaller subunits most notably biochemicals

A

macromolecules

132
Q

simple molecule that can be linked by chemical bonds to form larger molecules

A

monomers

133
Q

macromolecule made of a chain of repeating units Example: starch, protein, DNA

A

polymers

134
Q

compound containing primarily carbon, hydrogen and oxygen in 1:2:1 ratio

A

carbohydrate

135
Q

sugar; simple carbohydrate

A

saccharide

136
Q

simple sugar such as glucose that is a basic building block for more complex carbohydrates

A

monosaccharide

137
Q

sugar containing 2 monosaccharides Example sucrose= fructose + glucose

A

disaccharide

138
Q

carbohydrate that can be hydrolyzed into a number of monosaccharides Examples: cellulose, starch, glycogen

A

polysaccharide

139
Q

monosaccharide with 5 carbon atoms per molecule Example: arabinose, ribose, xylose

A

pentoses

140
Q

6-carbon sugar such as glucose and fructose

A

hexoses

141
Q

carbohydrate commonly referred as sugar, contain a 6-carbon structure

A

glucose

142
Q

fruit sugars

A

fructose

143
Q

sugars found in milk

A

lactose

144
Q

fermentable sugars formed from starch

A

maltose

145
Q

common table or cane sugar

A

sucrose

146
Q

bond that joins monosaccharides to form disaccharides and polymers

A

glycosidic bonds

147
Q

one carbon molecule gives up its OH group and the other loses the H from its OH group thereby producing a water molecule; happens during the formation of a carbohydrate bond

A

dehydration synthesis

148
Q

long, fibrous polymer composed of B-glucose (most common substances on earth

A

cellulose

149
Q

polysaccharide found in seaweed and commonly used to prepare solid culture media

A

agar

150
Q

network of polysaccharide chains cross-linked by short peptides that forms the rigid part of bacterial cell walls; gram negative bacteria have smaller amount of this rigid structure than do gram positive bacteria

A

peptidoglycan

151
Q

polysaccharide similar to cellulose in chemical structure. makes up the horny substance of the exoskeletons of arthropods and certain fungi

A

chitin

152
Q

molecular complex of lipid and carbohydrate found in eh bacterial cell wall. In negative gram bacteria is an endotoxin with generalized pathologic effects such as fever

A

lipopolysaccharide

153
Q

filamentous network of carbohydrate-rich molecules that coats cells

A

glycocalyx

154
Q

glucose polymer stored by cells

A

glycogen

155
Q

process in which water is used to break bonds in molecules; usually occurs in conjunction with an enzyme

A

hydrolysis

156
Q

lipid composed of glycerol molecule bond to 3 fatty acids

A

triglycerides

157
Q

3 carbon alcohol with 3 OH groups that serve as binding sites

A

glycerol

158
Q

compose a major structural component of cell membranes

A

phospholipids

159
Q

thin double-layered sheet composed of lipids such as phospholipids and sterols and proteins

A

membrane

160
Q

a conceptualization of the molecular architecture of cellular membranes as a bilipid layer containing proteins. Membranes proteins are embedded to some degree in this bilayer, where they float freely about

A

fluid-mosaic model

161
Q

best known member of a group of lipids called steroids; commonly found in cell membranes and animal hormones

A

cholesterol

162
Q

predominant organic molecule in cells, formed by long chains of amino acids

A

proteins

163
Q

building blocks of protein

A

amino acids

164
Q

covalent union between 2 amino acids that forms between the amine group of one and the carboxyl group of the other; basic bond of proteins

A

peptide bond

165
Q

molecule composed of short chains of amino acids

A

peptide

166
Q

initial protein organization described by type, number and order of amino acids in the chain

A

primary structure

167
Q

protein structure that occurs when the functional groups on the outer surface of the molecule interact by forming hydrogen bonds which cause the amino acids chain to either twist, forming a helix or to pleat in an accordion pattern called a B-pleated sheet

A

secondary structure

168
Q

protein structure that results from additional bonds forming between functional groups in a secondary structure creating a 3D mass

A

tertiary structure

169
Q

sulfide containing amino acid that usually produces covalent disulfide bonds in an amino acid sequence contributing to the tertiary structure of the protein

A

cysteine

170
Q

most complex protein structure characterized by the formation of large, multiunit proteins by more than one of the polypeptides; typical of antibodies and some enzymes that act in cell synthesis

A

quaternary structure

171
Q

protein biocatalyst that facilitates metabolic reactions

A

enzymes

172
Q

large protein molecules evoked in response to an antigen that interacts specifically with that antigen

A

antibodies

173
Q

nucleic acid; carries the master plan for an organism’s heredity

A

deoxyribonucleic acid (DNA)

174
Q

basic structural unit of DNA/RNA; consists of a phosphate, a sugar and a nitrogenous base

A

nucleotides

175
Q

nitrogen-containing molecule found in DNA and RNA that provide the bases for the genetic code

A

nitrogen base

176
Q

acidic salt containing phosphorus and oxygen that is an essential inorganic component of DNA, RNA and ATP

A

phosphate

177
Q

nitrogen bases that help form the genetic code on DNA and RNA

A

pyrimidines

178
Q

nitrogen base found in DNA and RNA with a purine form

A

adenine

179
Q

nitrogen base found in DNA and RNA in the purine form

A

guanine

180
Q

nitrogen base found in DNA but not in RNA in a pyrimidine form

A

thymine

181
Q

nitrogen base found in DNA and RNA with a pyrimidine form

A

cytosine

182
Q

nitrogen base found in RNA but not DNA in pyrimidine form

A

uracil

183
Q

5-carbon monosaccharide found in RNA

A

ribose

184
Q

5 carbon sugar that is an important component of DNA

A

deoxyribose

185
Q

nucleotide that is the primary source of energy to cells

A

adenosine triphosphate

186
Q

individual membrane bound living entity, the smallest unit capable of an independent existence

A

cell

187
Q

What is the difference between an atom and an ion?

A

atom has no charge while and ion has a charge

188
Q

What happens when you change proton, neutron, electron numbers of an element?

A

change neutron=isotope change protons= changes the element change electron=ion

189
Q

What is the # of electrons present in an atom called?

A

electron configuration Shell 1 can fill 2 electrons Shell 2 can fill 8 electrons

190
Q

What is the # in the outermost shell called and determines the degrees of reactivity and type of bonds it can make?

A

valence electrons

191
Q

What is the strongest bond?

A

covalent bond

192
Q

What is the weakest bond?

A

hydrogen bond

193
Q

What happens when an atom gains/loses electrons?

A

It becomes an ion forming ionic bonds

194
Q

What happens if the atom shares electrons?

A

forms covalent bonds

195
Q

charge attraction between 2 ions (cation & anion)

A

ionic bonds

196
Q

What is the difference between polar and non polar covalent bond?

A

electrons are not shared equally in polar covalent bond.

197
Q

What is a bond formed between 2 polar molecules?

A

hydrogen bond

198
Q

What is the attraction of an atom for the electrons in a covalent bond?

A

electronegativity

199
Q

What happens when an atom is more electronegative?

A

pulls shared electrons more strongly to itself

200
Q

Why is an ionic bond stronger than a hydrogen bond?

A

The attractions between the full charges of ions are stronger than the partial attractions found in hydrogen bonds.

201
Q

How do substances dissolve?

A

cations are attracted to the negatively charged oxygen and anions are attracted to the positive charged hydrogen in water molecules polarity of water allows hydrogen bonds with other polar molecules each ion becomes hydrated

202
Q

What types of substances can dissolve and why?

A

hydrophilic substances because their polar molecules or ions are water loving

203
Q

How to calculate pH of H?

A

pH=exponent Example: 10^2 pH= 2

204
Q

How to calculate pH of OH

A

pH= 14-exponent Example: 10^4 M 14-exponent (14-10=10)= 10 pH=10

205
Q

What are functional groups?

A

molecular groups that make up macromolecules

206
Q

Alcohols and carbohydrates are what functional group?

A

hydroxyl

207
Q

What functional group is carbon double bonded to oxygen?

A

carbonyl

208
Q

found in amino acids and acts as an acid (functional group)

A

carboxyl

209
Q

acts as a base (NH2 or NH3+), found in amino acids (functional group)

A

amino

210
Q

Functional group found in lipids

A

esters

211
Q

Functional group found in proteins

A

sulfhydryl

212
Q

Functional group found in DNA, RNA, ATP, high energy group

A

phosphate

213
Q

What are the 4 classes of biological molecules?

A

carbohydrates lipids proteins nucleic acides

214
Q

What are 3 types of lipids?

A

fats

phopholipids

steroids

waxes

215
Q

What are long term energy storage molecules in animals, insulation, wrap neurons increase conductivity and are composed of 1 glycerol covalently attached to 3 fatty acids?

A

Triglycerides

216
Q

How are amino acides classified?

A

Based on R group (side group)

nonpolar AA

Polar AA

Electrically charged AA

217
Q

Which bases are purine?

A

adenine

guanine

218
Q

What bases are pyrimidine?

A

Thymine

Cytosine

Uracil

219
Q

Thymine pairs up with ______ in DNA?

A

Adenine

220
Q

Adenine pairs up with ________ in RNA?

A

Uracil

221
Q

Cytosine pairs up with ________ in RNA/DNA?

A

Guanine

222
Q

What are the 3 types of RNA

A

message RNA: copy of a gene

transfer RNA: carrier that transports the correct AAs to the ribosome

ribosomal RNA: comprises the ribosome

223
Q

What is the structure of adenosine triphosphate (ATP) and function?

A

adenine

ribose

3 phosphate molecules

Releases energy when the bond is broken betwen the 2nd and 3rd phosphates

224
Q

culture

A

The visible accumulation of microorganisms in or on a nutrient medium. Also, the propagation of microorganisms with various media.

225
Q

A nutrient used to grow organisms outside of their natural habitats.

A

medium

226
Q

The implantation of microorganisms into or upon culture media.

A

innoculation

227
Q

Completely free of all life forms, including spores and viruses.

A

sterile

228
Q

What are the 5 Is in order?

A
  1. inoculation
  2. incubation
  3. isolation
  4. inspection
  5. identification
229
Q

What are the 3 types of media?

A

liquid

solid

semisolid

230
Q

What is the purpose of media?

A

to provide microbes with all of their required nutrients in an artifical medium

231
Q

water based solutions that do not solidify at temps above freezing, flow freely

Example: broths, milks, infusion

A

Liquid media

232
Q

Clot like consistency at room temp, thickens but no firm surface, used to determine motility of bacteria

A

semisolid media

233
Q

porivdes a firm surface upon which cells can form discrete colonies; used to isolate bacteria and fungi; can heat it up , liquefy it, cool it solidify it again

A

solid media

234
Q

What are the 3 types of hemolysins

A

gamma hemolysis

beta hemolysis

alpha hemolysis

235
Q

What type of hemolysin has no hemolysis?

A

gamma hemolysis

236
Q

What hemolysin complete lysis of red blood cells

A

beta hemolysis

237
Q

What hemolysin is an incomplete lysis of red blood cells

A

alpha hemolysis

238
Q

The greater the difference in composition between 2 substances, the great the _________ will be.

A

Refraction

239
Q

How does light travel?

A

Visible light travels in wavelengths, behaves as a particle called photons.

The smaller the wavelength the higher the energy.

240
Q

What are the 3 properties of an effective microscope?

A

magnification

resolution

contrast

241
Q

forms real image

A

objective lens

242
Q

forms virtual image received by teh eye and converted into a retinal or visual image

A

ocular lens

243
Q

Objective lens and ocular lens is used in________.

A

magnification

244
Q

capacity to separate 2 adjacent objects from one another (visually)

determined by a combo of characteristics of the objective lens and wavelength of light being used to illuminate sample

A

resolution

245
Q

Refractive index can be found in ___________.

A

Contrast

246
Q

most widely used type light microscope

ligth transmitted through speciment

specimen is darker than its surroundings

used for live, preserved, stained or unstained specimens

A

Bright-Field Microscope

247
Q

prevents light form entering objective lens from condenser

specimen is illumiatned, surrounding is dark

living cells cannot be heated, dried or stained

A

Dark Field Microscope

248
Q

Uses different density of parts of speciment to visualize intracellular structures

cell organelles are denser than cytoplasm

light patter varies in contrast

used to observe intracellular

A

Phase-Contrast Microscope

249
Q

bacteria stain purple

A

gram-positive bacteria

250
Q

bacteria stain pink

A

gram-negative

251
Q

detects peptidoglycan

gram + have peptidoglycan

gram - have no peptidoglycan

A

gram stain

252
Q

isolate a sample culture in a temperature controlled envrionment to encourage growth

A

incubation

253
Q

The term culture refers to the ______ growth of microorganisms in ___________.

A

macroscopic, media

254
Q

What is a mixed culture?

A

one that contains two or more known species

255
Q

Resolution is _________ with a longer wavelength of light.

A

worsened

256
Q
A
257
Q
A