Exam 1 - Lecture 1 Flashcards
What is Disease?
A disease is the result of a certain pathogenetic process that disrupts the well-being of an organism.
What is the process of disease?
infection and noninfectious
What is an example of a noninfectious disease?
aging
What is chronic disease?
A disease or health condition that lasts longer than three months, sometimes for the remainder of one’s life.
What is acute disease?
A disease or health condition that occurs and subsides within three months, usually within days or weeks.
What are examples of acute infection disease?
The cold virus infection (usually adenovirus) —causes inflammation in the mucous membranes lining the nose and throat.
Influenza—a viral infection of the respiratory tract.
Mononucleosis— Known as “the kissing disease,” common in teens; it’s a virus that multiplies in the lymphocytes.
What causes mono?
Epstein-Barr virus
What are the symptoms of mono?
Fever, fatigue, sore throat, atypical lymphocytes
What are rate of mono in children, teenagers, and adults?
childresn - 50%
teenagers - 35-50%
adults - 90-95%
mono in children
In children, infection with EBV usually cause no symptoms or disease is indistinguishable from the other mild, brief illnesses of childhood.
What is the EBV latency of mono?
EBV infects B-cells of the immune system and epithelial cells. Once the virus’s initial lytic infection (acute phase) subsides, EBV latently persists in the individual’s B cells for the rest of the individual’s life.
What are the EBV cells capable of?
Able to reactivate a virus and to shed viral particles
What is latent infection?
an extreme version of a persistent infection
Slow and abortive infectionsare more complicated variants of a persistent infection
What is EBNA-1 and 2?.
EBNA-1 is involved in promoting viral DNA replication
EBNA-2 is a transcription factor with viral and host cell targets
What is LMP1 and 2?
LMP1 expression in rodent cell lines results in transformation
LMP2 associates with src and several other tyrosine kinases
When is EBV under control of the host immune system?
in the chronic latent infection
When does immune supression occur?
autoimmune diseases, organ transplant
What causes post-transplant lymphoproliferative disorders?
reactivation of latent EBV
A child or a rare adult who was never infected before onset immuno-suppression lacks prior immunity, placing the patient at high risk for active viral infection and progression to neoplasia
Where is KSHV found?
Sarcoma kaposhi – found in patients who has HIV
Burkitt lymphoma
EBV if found in ALL cases of endemic Burkitt lymphoma, but many EBV infected people remain lymphoma free
What is the advantage of anti-apportial viral genes of EBV?
EBV’s anti-apoptotic viral genes provide a survival advantage to the lymphocyte that carries EBV
It expresses a number of alien genes (from EVB) and some host genes are expressed stronger than they should
What is the role of LMP1 in EBV?
-LMP1 expression in transgenic mice leads to B cell lymphoma; expression in fibroblasts leads to tumors in nude mice
-LMP1 accelerates B Cell Proliferation
-Upregulates adhesion molecules, CD23, CD40, IL-6, IL-10, etc.
-Activates NF-kB
LMP1 Inhibits apoptosis
-Upregulates Bcl-2, A20, Mcl-1
What is LMP1?
the EBV-Carried oncogene
What does LMP1 signaling stimulate?
Akt, NFκB, and Stat3 pathways
How can normals cells infected with viruses be transformed into cancerous cells?
from expression or activation or viral oncogenes
What can result from transformation?
transformation can result in integration of viral genes or genomes into the host genome
What are the classification of human disease?
chromosomal
single-gene
polygenic = complex = multifactorial
how do you find whether certain disease has a genetic component?
- Classic Family Study
- Twin studies
- Adoption studies
What are the 3 criterias of classical family study?
1) Identify the family
- ascertain all affected individuals
- sometimes difficult due to non-disclosure (especially in psychiatry)
2) Determine the proportion of the relatives affected (according to the degree of kinship to the index case)
3) Calculate the lifetime risk for morbidity for various relatives
What are the proportion of genes shared on the 3 degrees of relationship?
first - 50%
second - 25%
third - 12.5%
What are examples of first degree relationship?
parents and children
siblings and sibling
What are examples of second degree relationship?
grandparent and grandchild
uncle and nephew
half-siblings
What are examples of third degree relationship?
great grandparents and great grandchildren
first cousins
half uncles
how do you calculate the lifetime expectation morbid risk?
a = risk of siblings/population prevalence
What is the difference between mendelian and complex disease
Mendelian Diseases: Exhibit Mendelian mode of inheritance
Complex Diseases: Not Mendelian but Appear to Cluster in families
Why does family, twin, and adoption studies show greater risk to relatives of affected than population incidence?
that is simply because you share more genes with your relatives than with random stranger from the same population