Exam 1 for VTAH140 Flashcards
Before the nucleus
Pro-
Mostly composed of water and other components (i.e. enzymes, essential nutrients, proteins, carbohydrates, lipids, nucleic acids (DNA, RNA), ribosomes and waste products)
Cytoplasm
Responsible for holding all the components of the cytoplasm and it acts as a protective barrier.
Cell Wall
Consists of a double layer of phospolipids
Cell membrane
Aid in the transport of nutrients across the cell membrane -> the bacteria.
Structural Proteins
A structural polymer. Found within the cell wall.
Peptidoglycan
actual number & type of of these layers with the cell wall:
Differs between bacterial species
Contains the genetic sequence encoding all of the structural and functional components of a cell.
DNA
Generally have only one chromosome
Bacterial DNA
May contain additonal DNA
Plasmids
A circular piece of a double stranded DNA that is capable of replicating own its own, independent from the chromosome and not necessary for bacteria survival
Plasmids
Contain genes that encode for antibiotic resistance
Plasmids
Outer most protective layer made of polysaccharides
Capsule
Capsule is _______________:
antiphagocytic
The capsule of a bacteria cannot be eaten by:
macrophages and neutrophils
Bacteria having capsule makes ______
it harder to kill with disinfectants
unique feature if gram negative, found phospholipid layer of the outer membrane, and causes endotoxic shock
LPS
Generalized inflammation
Endotoxic Shock
surface appears smooth
Lots of LPS
surface appears rough
Little or no LPS
Bacteria that can assume more than one shape depending on environmental conditions
Pleomorphic
Single, Pair, Tetrad, Chain, Cluster
Common Bacterial Arrangements
Coccus, Cocobacillus, Rod (Bacillus), & Spiral (Spirochete)
Common Bacterial shapes
Some bacteria have it and is used for motility.
Flagella
single flagellum
Monotrichous
single flagellum at each end
Amphitrichous
bunches of flagella at one end
Lophotrichous
flagella all over
Peritrichous
long, hairlike structure, used to attach to colonize a surface, used for sexual reproduction, Not used for motility
Pili
tiny bristle- like structure distributed evenly over the entire surface, aid in attaching surfaces, Not used for motility
Fimbriae
Stain Blue and thick wall of peptidoglycan
Gram postive
Stain Red and Small amount of peptidoglycan
Gram negative
Some bacteria lack ______. A group that has a lipid-rich cell that contain _______ & ________. Resistant to most ______________. Group of bacteria are called _____________ genus
rigid cell walls; mycotic acids and complex lipids; staining dye; mycobacterium
Use sunlight to make food.
Photoautotrophs
Photoautotrophs examples
Plants and photosynthesis bactiera
Use inorganic substances (Ammonia and Sulfur) for food.
Chemoautotrophs
Chemoautotrophs examples
Extremophiles
Use organic substances (carbohydrates and fatty acids) for food.
Photoheterotrophs
Photoheterotrophs example
Heliobacteria
Use of chemicals that are consumed
Chemoheterotrophs
Chemoheterotrophs examples
Humans and Fungus
Bacteria reproduce ______ and need _______.
quickly; a lot of energy
Needed conditions _______, __, ________, and ________.
moisture, pH, temperature, and nutrients
Most pathogenic bacteria grow at
natural pH and at 37 C
presence of oxygen
Aerobic
absence of oxygen
Anaerobic
with or without oxygen (obtain oxygen from inorganic sulfates)
Facultative Anaerobic
low oxygen levels
Microaerophiles
requires 3% to 10% of Carbon dioxide
Capnophillic
Bacteria get energy from
Carbohydrates
Carboyhydrates are
Glucose, Lactose and Mannitol
Bacteria metabolize glucose by
glycolysis, krebs cycle, ETC
Glycolysis and Krebs cycle happens in the
Cytoplasm
Electron Transport Chain (ETC) happens in the
plasma Membrane
Bacteria uses _______ and _____ ____ to convert:
Glycolysis and Krebs Cycle to convert carbohydrates into energy to form (ATP)
Adenosine Triphosphate is
ATP
Is a chemical energy that is required for cells to live and without it the animal will die
ATP
A method of asexual reproduction
Binary Fission
- Parent cells duplicates the DNA and structures. 2. Parent cell grows. 3. Parent cell divides and the cross-wall is formed. 4. 2 identical daughter cells are formed (aka Clones)
Steps of Binary Fission
a process of binary fission of an single parent bacterium and its daughter cells. continues to repeat and results in the visible appearance of a bacteria colony. If provided optimal growing environment.
Clonal Expansion
What are the 4 stages of bacterial growth cycle?
Lag, Log, Stationary & Death Phases
no growth/adjustment period
Lag Phase
Period of maximum growth/exponential growth
Log Phase
limited availability of nutrients and an increase waste product concentrations
Stationary phase
Growth rate is equal to
the death rate
Depletion of available nutrients and increase in toxic waste products
Death Phase
a grouping of bacteria with a distinct architectural structure
Biofilm
Biofilm has two main parts
Core of bacteria and outer layer of polysaccharides
chemical signals that control the bacteria growth within the biofilm.
Quorom Sensing
resistant to phagocytosis
Antiphagocytic
resistant to destruction by antibiotics
Antibiotic resistant
a process where the bacteria goes through when the environmental conditions are bad and it develops an endospore
Sporulation
Develop within the bacteria and contains all the contents within the cytoplasm for survival
Endospore
Bacterial examples of Endospores
Bacillus Anthracis (Anthrax) & Clostridium
may occur following mutation in which a change occurs in the nucleotide sequence of a gene or recombination
Genetic Variation
results from mistakes in DNA replication. Can be good or bad.
Spontaneous mutations
_________________ induces an inheritable change due to the introduction of a new genetic material
Recombination
1 cell extends a sex pilus out to another cell, draws it closer, gives it a copy of a plasmid
Conjugation
Bacteria can scavenge naked DNA floating space, internalize it, and incorporate it into their own DNA
Transformation
Bacterial DNA îs transferred from one bacterial to another inside a virus capable of infecting bacteria (bacteriophages)
Transduction
the reduction of the number of pathogenic microorganisms to the point where they pose no danger of disease
Disinfection
chemical agent used on inanimate object to destroy microorganisms
Disinfectants
4 effectiveness of a disinfectant is dependent on:
Number of organisms present, pH, temperature, and concentration
Killing or remove of all microorganisms in a material or an object.
Sterilization
Chemical agent that is safe to use on living tissue to destroy microorganisms or to inhibit their growth.
Antiseptic
Time is required for the product to be effective
Contact Time
Level of effectiveness of a chemical antimicrobial
Potency
Kills the bacteria
Bactericial
inhibits the growth
Bacteriosatic
1st requirement in to disinfection is to start with a _________
Clean Area
___________ __________ i.e. of feces or dirt; protects the microbes against the antimicrobial products
Organic Matter
___________ removes organic material by increasing the wetting speed and breaking the organic material into small particles
Detergents
kills microorganisms by damaging cell components
Chemical antimicrobial agents
Chemical antimicrobial agents damage:
Proteins, Membranes, and other cell components
Bactericidal; not effective against spores or fungi
Alcohols
I.e. of Alcohols
Disinfecting Wipes
Bactericidal, Virucidal, Fungicidal
Accelerated Hydrogen Peroxides
I.e. of Accelerated Hydrogen Peroxides
Rescue
Contains glutaraldhyde and Antimicrobial Agent
Aldehydes
Used in chemical Sterilization
Aldehydes
Contains Dimethyl benzyl ammonia chlorides (BACs) or Benzalkonium Chloride, aka QUATS, Bactericidal and not effective against spores or fungi
Quaternary Ammonium Compounds
Contains Sodium hypochlorite (bleach) and its Bactricidal and Virucidal
Chloride
Contains 2% and it is Antiseptic/disinfectant - control a wide range of microorganisms such as bacteria and fungi
Chlorhexidine
Contains Iodine, Antiseptic/disfectant, and Antimicrobial to limit activity against bacterial spores
Iodine/Iodophors
all surface of the hands, fingers, and nails are scrubbed and contact time is 20 seconds
Hand washing technique
Areas commonly missed when washing hands:
Underneath finger nails, skin between fingers, and back of the thumb
free from contamination with harmful microorganisms
Aseptic
used to prepare for procedures under sterile conditions
Aseptic technique
Set of instructions, hand washing techniques, surgical gowns and gloves, and proper handling of sterile equipment and instruments
Standard operating procedure (SOP)
Methods used to sterilize
Heat, Radiation, Sonication
It penetrates thick material
Heat
Moist Heat example
Boiling Water
water that is pressurized to achieve a higher boiling point
Autoclave
Autoclave must maintain
15 psi and 121C for 15 min
Dry Heat example
Open/Oven Flame
use to sterilize metal objects and glassware
Dry heat
Considered sterilized for dry heat
171C for 1 hour or 121C for 10-16 hours
non-ionizing radiation example
UV lights
kills microorganisms at wavelengths of 200 nm
UV lights
Ionizing Radiation examples
X rays and Radioactive material
to sterilize plastic laboratory and medical equipment
Ionizing Radiation
use of ultrasonic vibrations to remove microbes from the surface of instruments
Sonfication
used to clean surgical or dental instruments
Sonfication
development and application of sanitary measures for the sake of cleanliness and protecting health
Sanitation
any substance of material that is composed primarily of carbon
Organic Material
Examples of organic Material
Feces, urine, soil, blood and pus