Exam 1: Example Test

Question 1

The process associated with DNA polymerase is

Answer 1

mitosis

Question 2

molecule required for meiosis and mitosis

Answer 2

DNA polymerase

Question 3

if you do not have this molecule you cant make microRNA

Answer 3

RNA polymerase

Question 4

microRNA, ribosome, mRNA, and tRNA are all required to make a

Answer 4

polypeptide

Question 5

microRNA, rRNA, mRNA, tRNA are all required for __ polymerase

Answer 5

RNA

Question 6

the process regulated by microRNA is

Answer 6

translation

Question 7

the process associated with RNA polymerase is

Answer 7

transcription

Question 8

the process interrupted by siRNA is

Answer 8

translation

Question 9

the polymerase chain reaction uses which molecule

Answer 9

DNA polymerase

Question 10

mRNA,cDNA, EST are all derived from a

Answer 10

transcript

Question 11

which of the following are not transcribed: mRNA, DNA polymerase, RNA polymerase, or ribosomal RNA

Answer 11

all are transcribed

Question 12

which process does NOT require complementary base pairing: replication, transcription, siRNA regulation, translation

Answer 12

all require complimentary base paring

Question 13

commonly used name for PCR machine

Answer 13

thermal cycler

Question 14

you have performed PCR and run your samples on a gel. when you look at the gel, you see very bright bands of DNA- what is true about this experiment?

Answer 14

there is ethidium bromide in the gel

Question 15

what could cause all transcription to stop in a prokaryote? A. the RNA polymerase gene has mutated B. a promoter sequence in front of the sole Gln-tRNA encoding gene has mutated C. a specific transcription factor is missing D. siRNA production has stopped E. All of the above

Answer 15

E. all of the above

Question 16

you have detected a 10-fold DECREASED in siRNA production in human cancer cells vs a control. What could this mean? A. overall RNA production has increased in the cancer subject B. a specific cellular control gene is no longer being silenced C. a group of cellular control genes are being silenced D. the cellular mechanisms controlling tRNA production may have been damaged E. DNA polymerase is being controlled by the siRNA

Answer 16

D. the cellular mechanisms controlling tRNA production may have been damaged.

Question 17

you have performed an extraction of a eukaryote cells’ RNA- you discover that there are no mRNA molecules- what is the most likely cause of this?

Answer 17

the RNA polymerase II genes have been inactivated

Question 18

you have performed a test on a patient and found that the mRNA of a suspect gene in the pathway of interest has been over-expressed, which of the following could be the cause?

Answer 18

trisomy

Question 19

you have performed a test on a patient and found that the mRNA of a suspect gene in the pathway of interest has been under-expressed

Answer 19

monosomy

Question 20

you have performed a test on a patient and found that the mRNA of a suspect gene in the pathway has a normal transcription level; which of the following could be the cause? A. siRNA is reducing polypeptide production B.microRNA is reducing polypeptide production C. the mRNA encodes a defective polypeptide D. either B or C E. all of the above

Answer 20

E. all of the above

Question 21

what could cause translation to stop in a cell?

Answer 21

a promoter sequence in front of the sole Gln-tRNA encoding gene has mutated

Question 22

you have performed PCR and run your samples on a gel. When you look at the gel, you dont see anything- what is the most likely reason(s)?

Answer 22

you “ran to black” and ran your samples off of the gel

Question 23

incorporation of amino acids into a growing polypeptide has stopped, which one of the following molecules is most likely lacking in a cell?

Answer 23

tRNA

Question 24

bacterial production of polypetide is drastically reduced. which of these is probably not forming?

Answer 24

polysome

Question 25

you notice a missing chromosome in a patients karyotype- which of these conditions might be caused by this monosomic condition?

Answer 25

turner syndrome

Question 26

which of the following represents the best possible (not likely to be random) "e" from a BLAST results? A. 0.15 B. 6.1 C.0.0 D. -150 E.-24

Answer 26

0.0

Question 27

which of these Hardy-Weinberg assumptions has CHANGED the most in Humans over the past 300 years?

Answer 27

Migration

Question 28

which of these Hardy-Weinberg assumptions has UNCHANGED the most in Humans over the past 400 years?

Answer 28

natural selection

Question 29

you wish to study a previously undiscovered organism. which data would give you a quick (within days) understanding of the organism to compare with other, known organisms?

Answer 29

Sanger DNA genomic sequencing

Question 30

The temp required to separate two complementary strands of the same DNA molecule is affected by which of the following factors? A. The A/T content of the strand B. the direction of the strand C. the A/C content of the strand D. the availability of DNA helicase E. the availability of DNA polymerase

Answer 30

A. the A/T content of the strand

Question 31

you have read a report that indicates your genome of interest has a 60% G/C content what is the percent of the genome that is thymine (T)?

Answer 31

20%

Question 32

in a diploid cell, mitosis leads to

Answer 32

two daughter cells

Question 33

you notice an extra chromosome in a patients karyotype- which of these conditions might be caused by this trisomic addition

Answer 33

patau syndrome

Question 34

in the scientific method, once you have identified a problem, what is the next step?

Answer 34

propose a testable hypothesis (solution)

Question 35

you have detected a 10 fold INCREASE in microRNA production in human cancer cells vs a control. what may have caused this increase?

Answer 35

a group of cellular control genes are being silenced

Question 36

you wish to study a previously undiscovered organism. Which data would give you a quick understanding o the organism to compare with other, known organisms? A. cDNA sequencing B. ITS sequencing C. EST sequencing D. mRNA sequencing E. all of the above

Answer 36

E. all of the above

Question 37

you have performed an extraction of a cells RNA- you discover that there are no tRNA molecules- what is the most likely cause of this?

Answer 37

the RNA polymerae III genes have been inactivated