Exam 1 (ch 1-4) Flashcards
Prokaryotic cells are found in
Archaea and bacteria
Eukaryotic cells are found in
Animals, plants
Historically a major tool until 1960’s , the primary technique
microscopy
What is the reason why most cells are small
They have an upper limit as to how big they can get
Breaks open the cell , releases the contents of it and then separating it into different components
Cell fractions
Separation of molecules and cell disruptions/Homogenizations
Cell fractions
Centrifugation separates based on what
Density
Electrophoresis separates based on what
Size, mass and charge
Most common method to determine 3D shape of proteins
X-ray diffraction
Used to produce populations of cells
Cell cultures
Used to study a function or process of interest choice
Model organism
Examples of model organism
E. coli, C. elegans(Nematode worm), a. thaliana, and zebra fish
A particle consisting of nucleic acid (RNA or DNA) enclosed in a protein coat
Virus
T/F: Viruses either have DNA or RNA , not both
True
Important characteristics of life includes:
Cellular organization, growth & metabolism, reproduction, heredity
3 German scientists that lead to the development of the cell theory
Schleiden, Schwann, and Virchow
based on work of Schledien, Schwann, and Virchow
Cell theory
Cell theory consists of 3 statements:
- All living organisms composed of cells. 2. Cells are the functional units of life. 3. Cells arise by division from pre-existing cells.
What year did Watson & Crick propose double helix model of DNA?
1953
In what year was the first draft sequence of human genome release?
2001
Rosalind Franklin was never credited at the time determining the structure of what?
DNA
All cells of some components in common:
Cell membrane, DNA & RNA, Cytoplasm, Ribosomes
Membrane surrounding cell; form outer most cell boundary for cells lacking a cell wall
Cell membrane (Plasma membrane)
Cell contents within the plasma membrane except the nucleus
Cytoplasm
Aqueous soln of the molecules that fills cytoplasm excluding all organelles
Cytosol
Discreet compartment of Eukaryotic cell surrounded by a membrane specialized in structure and function
organelle
What is the genetic material of all living organisms?
DNA
What is the only form of RNA translated into protein?
mRNA
Microbial colonization of tissues during what stage of life is important to the development of the mammalian immune system?
Infancy
Prokaryotic cells have been found in a range of what environment
Extreme environments
Any membrane limited subcellular structure found in Eukaryotic cells
Organelle
- contains most of cell’s genome(DNA)
- site of DNA synthesis (DNA rep)
- site of RNA synthesis (transcription)
nucleus
- energy metabolism
- fatty acid metabolism
- protein synthesis (small amounts)
mitochondria
- found in plants, fungi
- photosynthesis
- protein synthesis (small amounts)
chloroplasts
plants have mitochondria and chloroplast which both generates a high amount of
ATP
- synthesis of most lipids
- protein synthesis (on surface- rough ER)
- formation of ER vesicles for transport
endoplasmic reticulum (ER)
- modification of proteins (w or w/o sugar)
- formation of golgi vesicles for transport (also a network of the membrane)
golgi apparatus/complex
-intracellular degradation
lysosomes
degrades toxins
peroxisomes
-transport of endocytosed material, to lysomes
endosomes
uses part of the membrane that forms a vesicle that wraps around material and is brought into a cell
endosomes
the number of organelles and location within a cell may vary based on serval factors:
cell types, developmental stage, cellular energy status, etc
movement of a substance from a region of higher concentration to a region of lower concentration
diffusion
tends to even out concentration differences
diffusion
driving force of diffusion is simply the
concentration difference
diffusion is what kind of process?
spontaneous, does not require an input of energy
diffusion rate influenced by
- size of molecule/substance
- temp
- medium
T/F: diffusion is for large/long distance
false, it is for short distances
rate of diffusion would be slower or faster for larger substances vs smaller?
slower
increasing temperature increases or decreases the rate of diffusion?
increases
upper cell size determined by the ability of a material to enter and exit by
diffusion
diffusion of water (any solvent) through a membrane, in response to the concentration gradient “diffusion across a membrane”
-important cellular level process
osmosis
osmotically active substances include:
ions, proteins, sugars, etc.
hypertonic, isotonic, and hypotonic are terms used to describe what
osmotic concentration