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biotech csun > exam 1 > Flashcards

exam 1 Flashcards

1
Q

true or false? you need an electroporation cuvette for heat shock transformation of E.coli

A

false

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2
Q

Which of the following are parts of an adaptor used for preparation and sequencing of genomic DNA fragments

A) a sequence that anneals to an oligoneculeotide primer

B) a sequence that anneals to an sequencing primer to prime the sequencing reaction

C) a unique barcode sequence used to tag a genomic library

A

all of the above

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3
Q

TA cloning is a PCR based cloning true or false

A

True

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4
Q

you extracted RNA from a drought stressed plant leaf. you are interested in cloning only drought stressed cDNAs. what type of primer will you use at the very beginning after the sythesis of mRNA?

A

Gene specific primer

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5
Q

T4 polynucleotide kinase is used in Dr. Sanger’s method of DNA sequencing. true or false

A

false

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6
Q

in crisper system for use in biotech can the sgRNA and Cas9 gene be cloned in the same plasmid

A

yes

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7
Q

neoschizomers are restriction enzymes that

A

can bind at the same recognition site but cleave at differnt sites

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8
Q

a ddNTP has

A

a H group at the 3’ carbon

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9
Q

what is true about the oxidation step during primer synthesis

A

this step makes the bonds between the neucleotides stronger

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10
Q

at the time of the gene synthesis by primers how can you seal the nicks in the strands of DNA

A

by adding DNA ligase

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11
Q

is a chemical blocking group attatched to the 3’ carbon of the sugar moiety during pyrosequencing ?

A

no

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12
Q

what is true about capping during primer sythesis

A

capping is done to prevent prmers differing in lengths

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13
Q

luciferase+_____–> light

A

ATP

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14
Q

what is pyrosequencing?

A

a cleaved beta and gammma phosphate from dNTp molecule

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15
Q

MALDI-TOF analysis of protein molecules are represented by ?

A

mass to charge ratio

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16
Q

you want to repair the ends of DNA repair means the addition of nucleotides what type of enzyme do you use?

A

DNA polymerase

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17
Q

in colony immunoassay, first cells are lysed to release___ bound on the membrane

A

protein

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18
Q

you are identifying disease biomarkers with human protein microarray. what should be spotted onto the slide?

A

human proteins

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19
Q

what enzyme is used to “paste” your gene of intrest fragment into a plasmid cloning vector?

A

ligase

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20
Q

is DMT group part of a phosphoramidite?

A

yes

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21
Q

you are using “sandwich’ style protein microarray to study post translational modifications. should the proteins be labeled with flourescent tags?

A

no

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22
Q

you can analyze millions of transcripts using RNAseq? true or false

A

true

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23
Q

during primer synthesis how can you remove a DMT group?

A

by addition of TCA

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24
Q

in CRISPR invadiong foreign DNA have a sequence similarity with____

A

Crispr loci

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25
Q

which sequencing method has the lowest accuracy

A

Single molecule sequencing

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26
Q

the ccdB gene used in recombination cloning (gateway cloning) is a

A

gene that produces toxic compound

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27
Q

you extracted DNA then what is the first step in shotgun sequencing?

A

break genomic DNA

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28
Q

shine dalgarno sequence is

A

a ribosomal binding site in prokaryotes

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29
Q

in crisper system the invading DNA must have a

A

PAM sequence

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30
Q

in qPCR. if sample A has a Ct value of 20 and sample B has a Ct value of 40 in the same qPCR experiment can you canclude sample A has more DNA than sample B

A

yes

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31
Q

a kinase

A

transfers a phosphate group to a DNA

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32
Q

in blue white colony screening

A

LacZ gene encodes for beta galactosidase in the presense of X-Gal

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33
Q

activation and coupling during primer synthesis are mediated by

A

tetrazole

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34
Q

Dr. venter performed comprehensive construction of DNA libraries from all microorganisms from a region in Saragasso sea. this type fo study is known as

A

metagenomic study

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35
Q

During cDNA synthesis you extracted total RNA and by mistake added RNAseh instead of reverse transcript. what will happen?

A

my RNA will be degraded and i will not be able to continue cDNA synthesis

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36
Q

during the primer synthesis the purpose of adding a DMT group to a nucleotide is

A

to prevent unlinked nucleosides reacting non specifically

37
Q

you are using a yeast two hybrid system. you have 100 yeast strains containing DNA fragments as prey library. can you mix all prey yeast strains into a tube and have the bait yeast strains mate with the prey

A

yes

38
Q

your goal is to synthesize a gene. you synthesized oligonucleotides what should be now the immediate next step

A

increase temperature to 95*C for denaturation

39
Q

which of the following enzymes is able to synthesize another strand of DNA from a template DNA molecule

A

Klenow (DNA) polymerase

40
Q

the SYBR green dye used in qPCR can bind with both single and double stranded DNA

A

no

41
Q

what is true about metagenomic library construction

A

you need to perform shotgun sequencing

42
Q

what is detriylation?

A

the removal of DMT from necleoside during primer synthesis

43
Q

you can use pUC 19 for blue white colony screaning

A

true

44
Q

during synthesis of oligonucleotides the amino group is removed all nucleotides except

A

T

45
Q

find a restriction enzyme producing blunt ended DNA fragment

A

Pvull

46
Q

pyrophosphate +______ =ATP

A

ATP sulfrylase

47
Q

gel is needed for qPCR

A

false

48
Q

in crispr the components of single guide RNA is crRNA and _______

A

tracrRNA

49
Q

in tandem affinity purification procedure a plasmid DNA is used with two tags. which sequence is inserted between the two tags?

A

protease clevage site

50
Q

a 2D gel elecrephoresis system separates protein on the bands of elecgtric gradient an ____ gradien

A

pH

51
Q

what site/genetic element is an essential component of phage DNA to construct a phage library

A

COS

52
Q

what is the realistic proportion of mRNA in total RNA sample?

A

3%

53
Q

what is the role of SEC complex in protein transport in gram positive bacteria

A

creates a passage thx which entire protein and signal peptide passes

54
Q

what is the prinbrow box

A

TATAAT

55
Q

a bacteriophage life cycle goes through lysogeny and lytic cycle after it infects E. coli which is true?

A

in lysogeny phage DNA gets integrated in bacterial genome and maaintained in bacterial genome

56
Q

what are the number 5 and 3 in 5’ and 3’ represent when we describe the orientation of DNA

A

number on the carbon atom of the nucleotide

57
Q

biotin streptabin conjugate is used to detect numerous biomolecules including nucleic acids

A

true

58
Q

all of the antibiotics below inhibit protein sythesis and kill cells which one does not inhibit protein synthesis but still kills the cell

A

ampicillin

59
Q

at the time of labeling a probe it is nescessary to boil the probe. why?

A

boling denatures the probe and only a single stranded denatured probe can hybridize effectively with target DNA

60
Q

what is southern blot hybridization

A

the gene of intrest (probe) is labled and total genomic DNA is loaded onto the gel

61
Q

which enzyme is used to “paste” GOI fragment in a cloning vector

A

ligase

62
Q

find a restriction enzyme producing blunt ended DNA fragments

A

PvuII

63
Q

Neoshizomers are RE that can&raquo_space;»

A

bind at the same recognition site but cleave at a differnt site

64
Q

TATA box

A

promoter region

65
Q

RNA polymerase

A

binds to promoter region in the DNA to initiate transcription

66
Q

if a sequence of a template strand of a DNA is CAATGA the sequence of mRNA synthesized will be

A

GUUACU

67
Q

an mRNA molecule is successfully alternitively spliced to form two functional coding mRNA. alternitive splicing can generate two:

A

differnt types of mRNA with differnt exons

68
Q

arrange the following events in transcription and translation

RNA polymerase binds to DNA

ribosome is attatched to

to mRNA

mRNA sythesis

A

RNA polymerase binds toDNA -> mRNA synthesis-> ribosome is attached to mRNA

69
Q

and mRNA molecule has a unique feature which is absent in tRNA or rRNA

A

a poly a tail

70
Q

in blue white colony screening whats the role of IPTG

A

it inactivated repressor protein

71
Q

which of the following is not a part of the trnscription factor complex

A

TFIIZ

72
Q

as soon as proteins are synthesized from ribosome-mRNA complex the protein are loaded into the

A

rough endoplasmic reticulum

73
Q

what is the disadvantage of pBR322 cloning vector

A

very few cloning sites

74
Q

sometimes the functional genetic complementation is used at the time of gene cloning which is true of functional genetic complemntation

A

you can study gene response for host cell specific enzyme activity, make DNA library, transform host cells with DNA library

all of the above

75
Q

what is the smallest insert capacity to largest?

A

plasmids>bacteriophage lambda>BAC>YAC

76
Q

what does deoxyribose look like

A

2’H 3’ OH

77
Q

what is the finction of RNASeH

A

digest any type of RNA double or single stranded

78
Q

what is the disadvantage fo shittle vector

A

they can be lost from the host cell

79
Q

which one is the worst cloning vector for human genome sequencing

A

plasmid

80
Q

which is true of dynabead

A

have oligodT attached to it

81
Q

what is the role if kinase

A

transfer phosphate to donor molecules to substrates through phosphorylation

82
Q

is it posible for plant to have larger genome than humans

A

yes statment is true in some case but not all

83
Q

Which is true about klenow frag.

A

its a product of E. coli DNA polym

84
Q

Which is NOT true about pBR322 plasmid vector

A

has a lac z gene

85
Q

What’s true about gene cloning by functional complementation technique we studied in class

A

A. All correct; need normal and defective E. coli strain, need genomic DNA library w. Goi cloned, need to grow
cells on min. Medium

86
Q

Which enzyme is exclusively used in RNA manipulation but not in DNA manipulation

A

A. Poly A polymerase

87
Q

How do you develop EST library?

A

By random sequencing of cDNAs into a CDNA library

88
Q

You work for USDA and interested in gene expression in corn plants. You can perform

A

A. Microarray and northern blot

biotech csun (1 decks)

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