Exam 1 Flashcards

1
Q

What are the primary enzymes for protein digestion? In the answer, include their sources and products.

A

Pepsin: source- abomasum, produce- long chain amino acids, breaks down polypeptides to peptides
Rennin: source- abomasum, produces curds
Trypsin: source pancreas, breaks down- peptides to aa

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2
Q

Name 3 major overall functions of rumen microorganisms

A
  1. microbial protein production
  2. degradation of feedstuffs
  3. absorption of their substrate gas production
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3
Q

Name 3 bacteria important for ruminal fiber degradation; briefly explain

A

Fibrinolysin- breaks down fiber feeds
Cellulolytic- breaks down cellulose
Starch- Break down fiber feeds

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4
Q

Explain the functions of each compartment of the ruminant stomach

A

Rumen- storage, fermentation, microbial degradation and protein production
Reticulum- scratch factor, form feed bolus for rechewing and regurgitation
Omasum- Filter, doesn’t let too large of particles through and water absorption
Abomasum- true stomach, produces HCl for break down and digestion of feeds as well as other enzymes that acid in breakdown

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5
Q

Explain the importance of esophagus groove and how it works

A

The esophagus groove moves milk diectly to the abomasum, bypassing the rumen and reticulum. This allows majority of the nutrients to be broken down and absorbed in the small intestine. If the milk were to enter the rumen first, it would be less efficient and lactic acid would be produced in the rumen. The groove forms as the calf is suckling from both a physical and chemical response.

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6
Q

Give 3 examples of primary digestive enzymes for (A) protein, (B) carbohydrates, be specific about source, substrate and products of each

A

A. Pepsin- secreted in abomasum and breaks polypeptides down to peptides
Trypsin- secreted from the pancrease, breaks down peptides into the amino acids
Chymotrypsin- secreted from the pancrease, breaks down peptides into the amino acids
B. Amylase- secreted with saliva, breaks amylopectin down to glucose
sucrase- secreted in the small intestine, breaks down sucrose to glucose
Lactase- secreted in the small intestine, breaks down lactose down to glucose

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7
Q

Advantages vs disadvantages of ruminants vs. non-ruminants

A

Ruminants- utilize feedstuffs, can acquire nutrients from fiber, utilize feed of by-products, and regurgitate
Disadvantage- microbes are fed concentrate ruminants become less efficient as the microbes convert less of the feed to a form the animal can use

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8
Q

Describe the GI tract in ruminants, discuss anatomy and physiology of each section in proper order

A

GI tract begins at the MOUTH
MOUTH- with teeth designed to grind and regurgitate food
Saliva (introduced)- high water content introduced for lubrication and buffering of the rumen
Mouth to esophagus- binds to the rumen which is composed of four sacs and openly connected with the reticulum
Reticulum- this is the fermentation vat where microbes break down nutrients as particles of feed as particle of feed sort out with larger pieces being sent back up to the mouth for further grinding
Rumen- is lined with finger like projections that assist in absorption
Rumen to reticulum- feed is passed through the omasum for further absorption into the abomasum
Abomasum- has mucus lining to protect from HCl secretes, along with other enzymes.
Nutrients go into the duodenum where the low pH is buffered and more enzymes are added. The jejenum aid the illeum follow, which are the sites for most of the absorption. The cecum is where some ferementation and absorption happens. Large intestine is the last portion to move the fecal matter through the colon and out the animal

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9
Q

What is the importance of pancreas for digestion

A
Produces- enzymes responsible for
50%- carbohydrate digestion
50% protein digestion
90% lipid digestion
Produces- bicarbonate for neutralization of chyme in duodenum
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10
Q

What are the primary enzymes for carbohydrate digestion? In the answer, include their sources and products.

A

Maltose: (maltase) source- SI, produces- glucose
Lactose: (lactase) source- SI, produces- glucose and galactose
Sucrose: (sucrase) source- SI, produces- glucose and galactose

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11
Q

What are results in feeding ionophores?

A
  • increased propionate
  • reduced protein degradation
  • reduced deamination
  • reduced methane poduction
  • reduced lactate production
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12
Q

What is the reticular groove?

A

Reflex in a calf from the suckling process that is a chemical stimulation. The lips close and form a canal to bypass reticulo-rumen milk through omasal groove to the abomasum. This gradually stops functioning as the calf starts to eat solid food

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13
Q

How does saliva work in ruminants?

A

Function: provision of fluid for fermentation, alkaline buffering which is critical for rumen pH
feed, water and saliva are delivered to the reticulum through the esophageal, no digestive enzymes

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14
Q

What are the proximate analysis?

A
Moisture
Ash 
Crude protein
Ether extract
Crude fiber
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15
Q

What is the net effects of defaunation?

A
  • Increased daily gain
  • improved feed efficiency
  • Decreased OM and cellulose digestion
  • Increased total and microbial protein flow to the duodenum
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16
Q

What are 3 bacteria that are fibrolytic, briefly explain each

A

Ruminococcus spp.: major fibrolytic bacteria, needs NH3 for optimum growth, degradation of heavy forage
Butyrovibrio fibroslovens: substrates- starch, pectin, xylan, produce buyrate, demonstrates- proteolytic activity
Clostirdium spp: originate from silage, substrates- cellulose, starch, pectin, xylan, minor proteolytic

17
Q

What are the Adv. and Disadv. of protozoa?

A

Adv.
Increased cellulose digestion- 25-33% of total cellulose dig., mechanisms- provide more NH3 to bacteria, remove O2
Slower fermentation of starch and sugars
Greater VFA production

Disadv.
increased rumen protein turnover
increased CH4 production
development of more virulent strains of pathogenic bacteria

18
Q

What are some regulations of digestion

A

GI hormones/ peptides: Hormones secreted within GI tract affect digestion
Extrinsic/autonomic nervous system: Sympathetic- “fight or flight”, Parasympathetic- “Rest and digest”
Intrinsic/extrinsic nervous system: located in the GI tract- affects the digestive organs

19
Q

What are fermentation end- products of cellulolytic bacteria

A
Cellobiose
acetic acid
butyric acid
CO2 
H2
Lactic acid
20
Q

What are some factors that affect establishment of population

A

Presence of organisms: normally population is established through animal -animal contact, bacteria may establish without contact with mature ruminants
Favorable organisms: substrates and intermediates, increased rumen pH, digesta turnover

21
Q

How does diet alter the rumen population

A

High forage: High pH, cellulose, hemicellulose, sugars, high cellulolytic bacteria, high protozoa
High concentrates: low pH, high starch, low cellulolytic bacteria, high amylolytic bacteria, low protozoa

22
Q

If an animal was given ionophores what will happen?

A

Increases fibrobacter succinogens, prevotella rumicola, selenomonas ruminantium
Effects: decreases acetate, formate methane, decreases lactate, increased propionate

23
Q

Why are inoculants used?

A

reduce lactic acid build up difficult for long term- antagonistic environment, difficult to get enough organisms, considerable gene exchange

24
Q

Explain rumen fungi

A

fiber digestion
appear 8-10 days after birth
more prevalent on grasses than legumes, may be related to sulfur supplementation

25
Q

what are the mechanisms for microbial inoculants

A

transformation
conjunction
transduction

26
Q

What are the factors that depend on the type and amount of microbes

A

feed consumed- high forage and concentrate
max # in 2-3 high concentrate or 4-5 high forage
level of intake: high yields more
end products of fermentation are VFA, ammonia, gases

27
Q

What are the VFA’s and what do they do

A

Acetate: mostly from cellulose, milk fat synthesis, cellulolytic bacteria, energy source for rumen epithelium and muscle, not utilized in liver
Propionate: mostly from starch, produce glucose, amylolytic bacteria, utilized by rumen epithelium, precursor for gluconeogenesis
Butyrate: mostly derived from acetate, ketone usage as an energy source, metabolized by rumen epithelium to ketone bodies

28
Q

What happens with sudden dietary changes

A

Propionate to lactate; reduced pH
Lactate needs to be converted to pyruvate
Microbes converting lactate grow slow !!!!!
pH continues to drop
Too acidic environment
Lactic acidosis; can be lethal

29
Q

How is pH altered

A

Diet
Intake
Feeding frequency
Chewing/rumination

30
Q

What are regulation of mircrobial growth/function

A
Bacteria vs. protozoa
Competition
Environmental pH
Alteration with diet
Alteration with intake