Exam 1 Flashcards

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1
Q

carries information from DNA to protein synthesizing machinery

A

mRNA

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2
Q

transcribed from template strand of gene

A

mRNA

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3
Q

coding strand

A

5’ - 3’

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4
Q

template strand

A

3’ - 5’ , makes mRNA

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5
Q

RNA Polymerase

A

copies DNA sequence into RNA sequence during transcription. Don’t need primer.

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6
Q

DNA sequence where RNA polymerase attaches and initiates transcription

A

Promoter

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7
Q

sequence that signals the end of transcription

A

terminator

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8
Q

transcription factors and RNA polymerase II bound to the promoter

A

Transcription Initiation Complex

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9
Q

Differential Gene Expression

A

expression of different genes by cells with the same genome

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10
Q

cluster of proteins that assemble on the promoter sequence at the ‘upstream’ end of the gene

A

Transcription Initiation Complex

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11
Q

segments of noncoding DNA having particular nucleotide sequences that serve as binding site for proteins called transcription factors

A

Control Elements

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12
Q

protein + nucleic acid =

A

polymer

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13
Q

dehydration reaction

A

creation

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14
Q

hydrolysis

A

breakdown of polymer

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15
Q

enzymes

A

accelerate chemical reactions but are NOT consumed in reaction

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16
Q

3 parts to a nucleotide

A

nitrogenous base, sugar molecule (c, h, o), phosphate group

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17
Q

nucleotides are the _______ of DNA

A

monomers

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18
Q

What part of the nucleotide contains genetic information?

A

nitrogenous base

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19
Q

2 categories of nitrogen bases

A

Pyrimidines and Purines

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20
Q

Pyrimidines (Nitrogen Bases - 3)

A

Cytosine, Thymine, Uracil

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21
Q

Purines (Nitrogen Bases - 2)

A

Adenine, Guanine

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22
Q

What kind of sugar does DNA have?

A

deoxyribose (5 carbons + 2 OH)

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23
Q

What kind of sugar does RNA have?

A

ribose (5 carbons + 3 OH)

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24
Q

What does phosphodiester mean?

A
Phospho = phosphorus
Diester = bonded to oxygen and carbon
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25
Phosphodiester definition
chemical linkage between monomers (nucleotides in DNA or RNA). They are negatively charged
26
Chargaffs Rules
G = C A = T A + T + G + C + = 100
27
Watson & Crick
double helix model, antiparallel
28
____ is only in RNA
Uracil
29
8 functions of protein
Defensive, Enzematic, Transport, Storage (DETS) | Structure, Hormonal, Receptor, Contractile (SHReC)
30
How many TOTAL amino acids are there?
20
31
Acids are charged T/F?
True
32
Polypeptide (protein) synthesis occurs through a _________ reaction.
Dehydration
33
Primary Protein Structure
sequence of amino acids (pearl necklace)
34
Secondary Protein Structure
Alpha helix or Beta pleated sheet
35
Tertiary Protein Structure
Alpha helix and Beta sheet
36
What holds a tertiary protein structure together?
Van der Waals forces, hydrogen bonds, ionic bonds
37
3 levels of transcription
Initiation, Elongation, Termination
38
Nucleic Acid
polymers made of monomers. The monomers are called nucleotides.
39
What are the two types of nucleic acids?
Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA)
40
Gene expression
``` DNA = directions for replication and for RNA synthesis. RNA = controls protein synthesis ```
41
How many DNA molecules does each chromosome have?
1
42
Sites of protein synthesis are called _______.
Ribosomes
43
What does mRNA do?
conveys genetic instructions for building proteins.
44
Polynucleotides
many polymers, linear, make up nucleic acid.
45
The linkage of nucleotides into a polynucleotide involves a _______ reaction.
dehydration
46
sugar-phosphate backbone
Repeating sugar-phosphate units in a polynucleotide
47
DNA structures have __ polynucleotides, or 'strands'.
2
48
In a DNA double helix, the two sugar-phosphate backbones run ________.
antiparallel
49
RNA molecules have ____ strand(s).
1
50
tRNA
brings amino acids to the ribosome during synthesis of a polypeptide. 80 nucleotides in length.
51
______ is not present in RNA.
Thymine (T).
52
Transcription Factors
mediate the binding of RNA polymerase and initiation of transcription.
53
Virus
nucleic acid surrounded by a protein coat
54
Capsid
encloses viral genome
55
Bacteriophages
virus that infects bacteria
56
Virus-host range
limited number of host species that a virus can infect
57
Virulent Phage
only replicates in the lytic cycle
58
Temperate Phage
capable of using both cycles
59
Restriction Enzymes
'cut-up' phage DNA once recognized in bacteria (defense mechanism)
60
Retroviruses
transcribe RNA to DNA, backwards form of infection (HIV example)
61
Plasmids
thought to be one source of virus evolution
62
Transposons
thought to be one source of virus evolution