Exam 1 Flashcards
This gl is ø a specific pheromone producer gl:
Rabbit’s face gl.
Which is ø a method for genetic quality control of lab animals?
Back gross breeding.
Drawback of preference tests:
Animals choose in base of short term cond.
Which measurement is used to express E requirement of lab animals?
Metabolic BW.
Sentinels are:
Animals used to evaluate a grp’s health status.
Which method would you use to get 1mL of blood from a rat?
Cardiac puncture.
You cannot buy this type of diet from a commercial supplier:
Chemically defined diet.
ø alpha2- agonist:
Tolmetine.
ø inherited disease of rat:
Hypo- acidosis.
The basic method of rodent phenotyping is to look for:
Chromosoma test.
ø part of refinement of animal experimentation:
Alternatives to animal use.
Which animals are covered by 63/2010 EEc directive?
Vertebrates + Cephalipodes.
GLP ø concerned about cond. under which lab:
Are build.
Which sp. can die after oral penicillin treatment?
Guinea pig.
1 of them is an important mechanism of nutrigenomics:
Changing the signaling pathway.
Acute toxicity test has an invasiveness category of __ pain:
Severe.
Maintenance E requirements of lab animals:
- 45 MJ DE x W0.75.
This disease is ø a zoonosis:
Ringtail.
The recommended nutrient allowances:
Ensure the reproduction + longevity of animals.
Cannot be stage of mouse genital + urinary syndrome:
Ulceration.
Quarantine period ø determined by:
Number of animals.
Which sp. has a tied skin, so cannot grab it without causing pain to animal?
Guinea pig.
ø physical euthanasia method:
Maceration.
Which sp. is ø a member of Rodentia genus?
Marmoset.
OECD do ø deal questions about:
Validation of in vitro methods.
Gerbil is a good model for:
Epilepsy aorta.
The animal cannot be examined with US:
Golden hamster.
ø true for chronic mycoplasmosis of mice:
Zoonosis.
The closes sp. to Syrian hamster is taxonomy is:
Gerbil.
Good sp. to use for auditory research:
Chinchilla.
Breeding techniqure which is ø used to maintain an out- bred stock:
Brother- Sister mating.
A contaminated breeding colony ( line) should be:
Euthanised.
In reproductive toxicology, ø acceptable treatment:
Used only for parents before mating?
It can be used for intradermal inj:
Mice ear.
Pasteurella pneumotropica does ø attack:
Brain.
Environmental enrichment should ø be done:
Just for human fun.
How would your restrain a gerbil?
Hand around the chest.
Who will decide on institute level if an animal experiment was necessary or ø?
Ethical committee ( Animal Welfare Body).
It is ø a zoonosis:
Sendai virus disease.
This female embryo in a rat achieve breeding maturity:
1 mnth.
The basic method of rodent sexing is to look for:
Ano- genital distance.
The recommended nutrient allowances:
Ensure the optimum functioning of animals.
The main body of Lab Animal Science of the World:
CLAS.
The closest sp to Syrian hasmter in taxonomy is:
Gerbil.
What ‘ Environmental Enrichment’ means in lab?
To give bedding material/ toys to experimental animal if possible.
Feeding rancid feed and/ or vit E deficient feed may cause:
Dermatitis.
Zebradanio is:
Omnivore.
It is an alternative use of animals in experiments:
Database search + meta- analysis.
SC injury has a severity caategory of __ pain:
Minor.
Which 3 words called the 3 Rs by Russel + Burch?
Reduction, Replacemnet, Refinement.
It is ø true about euthanasia:
Can be used to get large amt of blood out of an animal.
What does ‘ knock out animal’ mean:
Trangenic animal.
Which method would you use to get a drop of blood from mice?
Retro- orbital puncture.
Which name indicates an outbred stock?
Ir: NMRI.
You cannot use this method for euthanasia on rat:
Ketamine.
What is the best equipment to maintain a trangenic colony in?
Base colony in different countries.
Which sp. has only provoked ( stimulated) ovulation?
Ferret.
What does the expression ‘ inbred strain’ mean:
Brother- sister mating through at least 20 generations.
Which sp. is the best in atherosclerosis research?
WHHL- rabbit; Notes- Gerbil.
You can find a ‘ sacculus rotundus or ampulla ilei’ in the:
Rabbit.
This gl is a specific pheromone producer gl:
Hamster’s lat gl.
Which sp has a gallbladder?
Golden hamster.
What does SPF mean?
Specific Pathogen Free.
How would you restrain a rat for /oral dosing?
By grabbing the skin on abck + neck.
Which sentence is ø true about ‘ Environment Enrichment’ ?
Device in case that animal can control.
Gnotobiotic ( = Axenic) animals:
Germ free.
What does rederivation mean?
Production of germ- free strain by embryo transfer.
In the practice, it is ø possible to produce germ- free colony from this sp:
Duck.
Which would be the best feeding regime in an experiment?
Ad lib.
What is well- being?
State of physical + psychological harmony of animal with it’s int + ext environment.
Which vein can be used for IV inj on ferret?
Jugular vein.
How would you restrain a rabbit?
By grabbing as much skin as possible on back.
You cannot use this method for euthanasia on new-born, nest- living animals:
CO2 inhalation.
Which method would you use to get 10 mL of blood from an adult rat?
Preparation of abd aorta.
Which mouse strain is sensitive to Pneumocystis carini?
Swiss.
Which is ø necessary before transport of rodent?
Fasting.
When this phenomenon disappears, the narcosis ends with death:
Cornea reflex.
It is ø true about euthanasia:
Should be cheap.
1 is ø true about the co- isogenic strains:
It can only be maintained in a homozygous form.
Dog is ø a good model for:
Cardiovascular atherosclerosis.
Mini- Sus has a live weight of:
20 - 45 kg.
Experimental design cannot be:
Cross- breeding.
The basal circadian rhymth, is:
Dependent from the gen expression of a protein.
Melatonin is produced by:
Pineal gl.
This sp mostly used in viral respiratory behaviour:
Apathy/ Aggression.
Well- being cannot be measured by:
- Clinical assessment.
- Physiological parameters.
- Grimace score.
- Reproductive state.
Which environment is good for animals?
Classical music.
Which tissue is the least sensitive to suffering?
Brain tissue.
The naked mole rat has an extra- ordinary long lifespan because of:
Special hyaluronan which prevent cancer.
The co- efficient on inbreeding ( F):
Means the franction of genes that get fixed in a homozygous state.
It is inherited disease of some mouse strains:
Deafness.
Which is a member of Poxviridae?
Ectomelia virus.
Ringtail is caused by:
Too low relative humidity.
ø part of refinement of animal experimentation:
Hair colour.
The eharing range of rodents:
0.5/ 1 - 100/ 120 kHz.
Which sp has 1 pair teats?
Guinea pig.
The 2 important words for animals concerning the pain feeling ( Weiss- trail):
Predictability + controllability.
Mousepox is
Caused by Ectomelia.
It is ø physical euthanasia method:
Cooling in refrigerator.
The African clawed for ( Xenopus):
Shows mating behaviour after Hcg inj.
The axolotl is ø:
Reaches adulthood after a complete metamorphosis.
Lab animals- except the guinea pig- usually adjust their feed intake to satisfy their:
E requirement.
Which sp can die after oral Ab treatment?
Guinea pig + rabbit.
The role of PPAR is to:
Burn fats.
1 of the following has ø anti-cancer effect:
Fe.
1 of them is an important mechanism of epigenomics:
Methylation of DNA.
Zebrafish has a lifespan of __ year:
6 - 8.
1 of them has ø role in epigenomics:
Change of base in DNA.
The role of __ is a fine regulation of protein synthesis in cell:
miRNA/ siRNA.
In some cases the epigenetic changes may be inherited:
IF the effects act during the germ- cell proliferation ( ‘ time window’).
You can find neotomy at:
Axolotl.
Pheromones are detected by:
VNO.
1 is true about the ferret:
We can prevent the const estrus by using GnRH agonist implants.
The cephalopods:
are under the protection og 2010/63/EU Directive since 2013.
Always consider using F1 hybrid because:
- Their biological response is more variable than the parental strains nor their biological response is more uniform than the parental strains.
Neither.
1 of the statements is true:
Animals in an outbred stock are anisogenic.
A chromorhnydachyrrhea:
Vit A deficiency.
It can go through the uteral wall:
Mouse mammary tmour.
- LCM.
CH ingestion:
Stimualte the FA synthase gene expression.
May increase the mitochondria number:
Resveratrol.
The most used model for drug metabolism study for humans:
Macaco.
Shortly describe the pyramide conception, with e.g:
- Computer- Synthetize compounds.
- Bacteria- Test for teratogenicity.
- Cell culture- Eliminate harmful compounds.
- Invertebrates, Fish, Mammals, non- human primates.
- Human.
Explain the epigenetics mechanism of action.
- Modifications.
- ø change the genome itself.
- May be inherited.
- Influenced by outside stimuli.
Describe the naked mole as an animal model.
- Have a gene which conserves collagen.
- ø age + get tumors.
- Lifespan of 20 - 30 yrs.
- Complex social system + hierarchy.
- Best model for aging + cancer studies.
Weis experiments: 2 series of trials.
- Showed corticothalamic blunting - “ stress analgesia”.
- Production of endogenous opioids in stressful situations which increases the pain threshold.
- 1st trial: 3 grps of rats.
> Received light, then 10s afterwards received an electric shock to tail; went on for 1hr.
>Received light + electric shock before + after, ø set timing.
> ø light, ø shock ( control grp). - Then checked for physiological state.
> Grp 3 was normal ( control).
> Grp 1 was also normal because they had time to anticipate pain + produce endogenous opioids + increase their pain threshold
> Grp 2 was miserable + in pain. - 2nd trial: + grp 3, same experiment but received a switch to stop the electric shock.
> the results were the same, because grp 1 could anticipate the shock and flip the switch.
Explain the pain scoring system for Glires.
- 0 pts= none.
- 1 pt= Mild.
- 2 pts= Moderate.
- 3 pts= Severe.
- Based on appearance, behaviour, pulse, appetite/ drinking, cardiovascular, digestive + nervous systems.
- 0 - 4= normal.
- 5 - 9= needs analgesia.
- 10 - 14= moderate pain, possible need to terminate trial.
- 15- 20= ø physiological, stop experiment.
