Exam #1 Flashcards
Define microbiology?
The study of microorganisms
What are microorganisms?
cellular and noncellular organisms that are too small to be seen by the naked eye
Where do you find microorganisms?
on things where they can eat so that they can grow and divide
How many microorganisms are estimated to be on Earth?
25 x 10^29
How long have microorganisms been on Earth?
4 billion years
How do microorganisms impact our lives through disease?
cause and treatment
How do microorganisms impact our lives through agriculture?
microbes help plants absorb the nutrients they need
How do microorganisms impact our lives through food?
beer, yogurt, cheese; microbes are used to make a lot of our food; call also spoil them
How do microorganisms impact our lives through energy?
biofuels; microbes can help convert different plants into enthanol
How do microorganisms impact our lives through biotechnology
use of microorganisms in industrial biosynthesis, typically by microorganisms that have been genetically modified to synthesize products (ex. Insulin) of high commercial value
Name 6 abilities/characteristics of living cells.
metabolism, reproduction, communication, differentiation, movement, and evolution
Who was the first to use a microscope to see microorganisms?
Robert Hooke
What did Robert Hooke see?
cork cells
What type of microscopy did Robert Hooke use?
glorified magnifying glass
Who was the first to use a microscope to see bacteria?
Van Leeuwenhoek
How do we know that Van Leeuwenhoek saw bacteria?
he drew and took pictures
What type of microscopy did Leeuwenhoek use?
magnification
List at least 4 ways microscopy is useful
allows cell shape and arragnement to be seen, structures to be seen, pathogens to be seen among animal cells, and distinguish gram-positive from gram-negative
What are the four types of light microscopy?
brightfield, phase contrast, darkfield and fluorescent
What must be done to a microorganism to be able to see it with brightfield light microscopy?
must be stained and only uses light source and lenses
Which types of light microscopy allow you to view a live sample?
Phase contrast and Darkfield
What is the maximum resolution of a compound light microscope?
0.2 microns
Describe what is meant by the resolution.
this means that objects clsoer than 0.2 microns cannot be resolves as distinct and separate
What are the two types of electron microscopy?
Transmission Electron Microscope and Scanning Electron Microscope
What is Transmission Electron Microscope?
for observing internal cell structure
What is Scanning Electron Microscope?
for 3D imaging and viewing surfaces
What is the maximum resolution of an electron microscope?
0.2-0.4 nm
What is the smallest microbial cell size discovered thus far?
0.2 microns
Would a cell that is 0.2 microns have a high or a low surface area-to-volume ratio compared to an average eukaryotic cell?
high surface area-to-volume ratio
What is the significance of a cell having a high surface area-to-volume ratio?
aids in nutrient and waste exchange with the environment
Discuss the problems a cell might have if it were less than 0.2 microns.
smaller than this doesn’t allow them to house everything they need to make a living cell
What microorganisms have a phospholipid bilayer form their cytoplasmic membrane?
Bacteria and Eukarya
What microorganisms have a lipid monolayer form their cytoplasmic membrane?
Archaea
What does a phospholipid bilayer made of?
fatty acid, glycerol, phosphate, and ester linkages
What does a lipid monolayer made of?
glycerol and ether linkages
What advantage would microorganisms with lipid monolayer have over those with a lipid bilayer?
more resistant to peeling apart than bilayers, no hydrophobic interaction, and stronger than bilayer
What are the functions of the cytoplasmic membrane?
permeability barriers, protein anchor, transport, and energy conservation
What is meant by permeability barriers?
prevents leakage, functions as a gateway for transport of nutrients into and wastes out of cell
What is meant by protein anchor?
site of many proteins that participate in transport, bioenergetics and chemotaxis
What is meant by energy conservation?
site of generation and use of the proton motive force
What are the three classes of transport systems?
simple transport, group translocation, and ABC transport
What is simple transport?
driven by the energy in the proton motive force, one in and one out