Exam 1 Flashcards
what is microbiology
study of microorganisms or microbes which are often invisible to the naked eye
The term microbe encompasses
cellular, living microorganisms like bacteria, archaea, fungi, protists, and helminths
non-living microbes
viruses and prions
Spontaneous generation
life comes from nonliving items
biogenesis
life emerges from existing life
proof spontaneous generation exists
Francesco Redi- the first person to challenge theory - meat in an uncovered jar (maggots) - meat in a covered jar (no maggots)
Louis Pasteur
showed biogenesis is responsible for the propagation of life – pasteurization killed off yeast and prevented stored wine from turning bitter
Prokaryotic organisms
ALL are unicellular, lack a nucleus, 2 main categories: bacteria and archaea
Eukaryotic organisms
Unicellular or multicellular, distinct nucleus, 4 main types: animal, plants, fungal, protist
Binomial nomenclature system
Genus is first name (capitalized), species is the second name (lowercase)
Taxonomic hierarchy
domain, kingdom, phylum, class, order, family, genus, species
Domain
bacteria, archaea, eukarya
older 5-kingdom
amimalia, plantae, fungi, protista, monera
Prokaryotic cell structure
have a nucleoid, lack membrane-bound cytoplasmic organelles, use binary fission
binary fission
parent cell – replication of DNA – segregation of DNA – cell splitting into two
flagella
involved in motility – spin like propellors to move cells, numbers and arrangements help with characterization
flagella 2
have the capacity to swim freely through and aqueous habitat, act as sensory organs to detect temp. and pH changes
chemotaxis
Bacteria sense chemicals and move accordingly
aerotaxis
towards higher oxygen content
thermotaxis
towards heat
phototaxis
towards light
fimbriae
are used for attachment- gram-negative bacteria- E.Coli
pili
are used for motility and transfer of DNA- gram-negative DNA
DNA transfer of pili
called conjugation, sex pilus extends from one bacterium to another, DNA transfer can add new functions to the recipient cell
glycocalyx (bacteria external structures)
Gel-like layer outside cell wall and protects or allows attachment
Biofilms
free cells adhere to the surface and multiply, releasing polymers, nutrients, and wastes pass through characteristic channels, communicate via chemical signals
S-layers
are the outmost cell envelope component, composed of single protein or glycoprotein species
S-layer protein functions
biogenesis of the cell wall, control of cell division, interfere with the immune system, and can aid survival via adhesion
gram-positive bacteria
peptidoglycan cell wall, then plasma membrane, purple stain, contains glycopolymer links cell wall to plasma membrane
gram-negative bacteria
outer membrane and think peptidoglycan layer, then plasma membrane, pink stain, contains lipopolysaccharides (help bacteria survive in the gut of animals
cell wall
made of peptidoglycan, alternation series from glycan chains (NAM and NAG), tetrapeptide chain (string of 4 amino acids) links glycan chains
acid-fast bacteria
have cell walls made up of primarily long-chain fatty acid – mycolic acid
bacteria internal structure
Cytoplasm (PM), genetic material (chromosomes, plasmids), ribosomes, inclusion bodies, endospores
cytoplasmic membrane
Phospholipid bilayer embedded with proteins – proteins serve as selective gates and sensors of environmental conditions – selective permeability
ribosomes
in charge of protein synthesis
Archaea
live in extreme environmental conditions, common in swamps, mud, digestive tracks of animals, require salt to grow, use pigment to synthesize ATP
archaea cell wall
can be composed entirely of polysaccharides or pure protein– NO peptidoglycan
Eukaryotic cells cytoplasmic membrane
contain sterols – cholesterol (mammals) and Ergosterol (fungi)– they provide strength to fluid structure, phospholipid bilayer embedded with proteins
endocytosis
cell takes up material from the surrounding environment
takes in something from outside
exocytosis
internal vesicles fuse with cytoplasmic membrane and release contents
cell pushes something out
action filaments (cytoskeleton)
allow movement
microtubules (cytoskeleton)
framework for organelle and vesicle movement
Intermediate filaments (cytoskeleton)
provide mechanical support
nuclear pores
allow large molecules to pass
nucleolus
is the region where ribosomal RNAs are synthesized
Lysosomes
contain degradative enzymes
known as “garbage disposal” – break down waste material
peroxisomes
degrade lipids and detoxify chemicals
microbial growth
binary fission, budding (chromosome duplicated and place in the bud, separation occurs, spore formation (sexual or asexual)
Lag phase ( Growth curve)
number of cells do not increase and cells begin synthesizing
log phase (growth curve)
cells divide at constant rate and produce primary metabolites (amino acids) and secondary metabolites (antibiotics)
stationary phase (growth curve)
nutrient levels too low to sustain, total numbers remain constant
death phase (growth curve)
total number of viable cells decreases, cells die at a constant rate
center of colony
depleted O2 and nutrients, accumulation of potentially toxic wastes
edge of colony
little competition for O2 nutrients, cells at the edge may show exponential growth
process of growth after inoculation
culture
Media types by physical state
Liquid, semi-solid, solid
general purpose of media types
to grow as many potential microorganisms as possible
enriched medium purpose of media types
contains complex organic substances or growth factors to grow microorganisms with special growth requirements
selective media purpose
grow specific microorganisms, permitting rapid identification of a genus or even species of a target organism
differential media types purpose
allows multiple types of microorganisms to grow but are designed to display visual differences among colonies
aerobic microorganisms
Microorganisms that can survive and grow in oxygenated environments
microaerophilic microorganisms
require lower oxygen concentrations
anaerobic microorganisms
any microorganism that does not require oxygen to grow
microbe isolation
process of separating one species from another
direct cell counts: detecting microbial growth
total numbers (living and non-living)
turbidity: detecting microbial growth
the cloudiness of liquid due to bacteria, measuring cell mass instead of numbers
3 properties that microscopes have
magnification, resolution, contrast
magnification
apparent increase in size
resolution
Distinguishing magnified objects clearly
contrast
determines how easily cells can be seen
Microscopes available
light microscope (most commonly used in microbiology), electron microscope, scanning probe microscope