Exam 1 Flashcards
Tissue samples placed in solutions of chemicals that will preserve the cell and tissue structure?
Fixation, Step 1 (preparation)
The tissue is placed in a cassette, then transferred through a series of concentrated alcohol to remove water from the specimen?
Dehydration, Step 2
(preparation)
The alcohol used in the dehydrating step is removed using organic solution miscible with alcohol and paraffin?
Clearing, Step 3
(preparation)
The sample and cassette are placed in paraffin wax or imbedding medium until it becomes infiltrated with the substance?
Infiltration, Step 4
(preparation)
The sample is then embedded in a mold containing melted paraffin and allowed to cool to harden the paraffin?
Embedding, Step 5
(preparation)
The paraffin block is then trimmed to expose tissue for sectioning using a microtone. Microtone shaves off thin sheets of block for slide, then stained and examined. Sheets then floated on water and picked up on a slide before staining?
Trimming, Step 6
(preparation)
After the slide with tissue samples is stained, it will be preserved by adding a protective glass coverslip over the tissue with a clear adhesive?
Mounting, Step 7
(preparation)
Dyes stain materials _____
they act like acids and bases to form electrostatic linkages with macromolecules in tissue?
selectively
A basic dye that binds to acidic components of a tissue?
Hematoxylin
Which dye has a positive charge and will stain negatively charged structures blue?
Hematoxylin
An acidic dye that binds to basic components of a tissue?
Eosin
Which dye has a negative charge that will stain positive charged structures pink to red?
Eosin
Hematoxylin sticks to ___ structures (AKA acidic or negatively charged structures) and dyes them ___?
Basophilic, Blue
Eosin sticks to the ___ structures (AKA basic or positively charged structures) and dyes them ___?
Acidophilic, Red
In thick skin, ___ stains pink with eosin?
Collagen
In thick skin, ___ ___ will stain purple with hematoxylin?
Cell nuclei
Giemsa-Wright Stain dies red blood cells what color?
Red
Giemsa-Wright Stain dies white blood cells what color?
Purple
Silver stains are great for ___?
Fungi
___ ___ stain with silver in a lymph node?
Reticular Fibers