Exam 1 Flashcards

1
Q

Tissue samples placed in solutions of chemicals that will preserve the cell and tissue structure?

A

Fixation, Step 1 (preparation)

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2
Q

The tissue is placed in a cassette, then transferred through a series of concentrated alcohol to remove water from the specimen?

A

Dehydration, Step 2
(preparation)

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3
Q

The alcohol used in the dehydrating step is removed using organic solution miscible with alcohol and paraffin?

A

Clearing, Step 3
(preparation)

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4
Q

The sample and cassette are placed in paraffin wax or imbedding medium until it becomes infiltrated with the substance?

A

Infiltration, Step 4
(preparation)

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5
Q

The sample is then embedded in a mold containing melted paraffin and allowed to cool to harden the paraffin?

A

Embedding, Step 5
(preparation)

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6
Q

The paraffin block is then trimmed to expose tissue for sectioning using a microtone. Microtone shaves off thin sheets of block for slide, then stained and examined. Sheets then floated on water and picked up on a slide before staining?

A

Trimming, Step 6
(preparation)

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7
Q

After the slide with tissue samples is stained, it will be preserved by adding a protective glass coverslip over the tissue with a clear adhesive?

A

Mounting, Step 7
(preparation)

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8
Q

Dyes stain materials _____
they act like acids and bases to form electrostatic linkages with macromolecules in tissue?

A

selectively

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9
Q

A basic dye that binds to acidic components of a tissue?

A

Hematoxylin

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10
Q

Which dye has a positive charge and will stain negatively charged structures blue?

A

Hematoxylin

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11
Q

An acidic dye that binds to basic components of a tissue?

A

Eosin

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12
Q

Which dye has a negative charge that will stain positive charged structures pink to red?

A

Eosin

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13
Q

Hematoxylin sticks to ___ structures (AKA acidic or negatively charged structures) and dyes them ___?

A

Basophilic, Blue

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14
Q

Eosin sticks to the ___ structures (AKA basic or positively charged structures) and dyes them ___?

A

Acidophilic, Red

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15
Q

In thick skin, ___ stains pink with eosin?

A

Collagen

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16
Q

In thick skin, ___ ___ will stain purple with hematoxylin?

A

Cell nuclei

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17
Q

Giemsa-Wright Stain dies red blood cells what color?

A

Red

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18
Q

Giemsa-Wright Stain dies white blood cells what color?

A

Purple

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19
Q

Silver stains are great for ___?

A

Fungi

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20
Q

___ ___ stain with silver in a lymph node?

A

Reticular Fibers

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21
Q

___ ___ ___ stains carbohydrates deep red?

A

Periodic Acid Schiff (PAS)

22
Q

PAS stains carbohydrates and rich ___?

A

macromolecules

23
Q

PAS will stain ___ in intracellular storage?

A

Glycogen

24
Q

What stains mucus, basement membranes, brush boarder of kidneys and intestines, reticular fibers in connective tissue and cartilage?

A

Periodic Acid Schiff (PAS)

25
Q

Toluidine blue will stain most things blue but mast cells will stain ___?

A

Purple

26
Q

A condition where there is a mast cell burden in areas of the body such as skin, bone marrow, GI tract, and other places?

A

Mastocytosis

27
Q

The biggest cells are ___ ___ at 30 micrometers?

A

Motor Neurons

28
Q

Most cells are between ___ and ___ micrometers?

A

10 and 30

29
Q

Red cells are about __ micrometers?

A

7

30
Q

Cell shapes vary from ___ to _____ to flat?

A

Round , Hexagonal

31
Q

Do nuclear shapes and locations vary?

A

Yes

32
Q

What are the levels of the Histologic Hierarchy?

A
  1. Cells
  2. Tissues
  3. Organs
  4. Organ Systems
33
Q

Tissues are composed of___ and ___?

A

cells, extracellular matrix

34
Q

Organs are composed of ___ and ___?

A

parenchyma, stroma

35
Q

The cells that perform the main function of an organ?

A

Parenchyma

36
Q

Supporting tissue of organs?

A

Stroma

37
Q

What are the functions of Epithelium?

A

Cover external surfaces, line internal surfaces, protect, absorb, secrete, sensation, contraction

38
Q

What are the two main kinds of epithelium?

A
  1. covering and lining epithelium
  2. glandular epithelium
39
Q

what do covering and lining epithelium cover?

A

outer surfaces of the body and stratified squamous epithelium in the vagina

40
Q

what is the function of glandular epithelium?

A

contains specialized cells for secretion (ex: active mammary glands)

41
Q

____ anchors epithelium to underlying connective tissue?

A

basal lamina

42
Q

Epithelial cells are very cohesive due to ____?

A

intercellular junctions

43
Q

Epithelial cells vary a lot in ___ and ___?

A

shapes, sizes

44
Q

____ tissues are avascular?

A

Epithelial

45
Q

____ cells demonstrate polarity?

A

Epithelial

46
Q

What constitutes the structure of epithelium?

A

basement membrane, connection between cells and specialized apical structures

47
Q

The basement membrane is made of the ___ lamina and the ___ lamina?

A

basal, reticular

48
Q

The basal lamina connects directly to ___ tissues?

A

epithelial

49
Q

what are the two layers making up the basal lamina?

A

lamina lucida (mostly laminin = glycoprotein)
and
lamina densa (type IV collagen and perlecan= proteoglycan)

50
Q

The reticular lamina connects the basal lamina to ____ ____ ____?

A

underlying connective tissue

51
Q

The reticular lamina is made up of what 3 layers?

A