Exam #1 Flashcards
1cm= how many mm?
10 mm
1mm= how many microns?
1000 microns
Eyepiece (ocular lens)
Magnifying lens that enlarges the microscopic image 10x. The piece that you look in.
Rotating Nosepiece
Holds several objective lenses of different magnifications. By rotating it, one can change the magnification of the microscope.
Objective Lense
They magnify the image coming directly from the object. There are 3 objective lenses: scanning(4x), low power(10x), and high power(40x).
Focal Adjustment Knobs
These adjust the focus of the microscope. They alter the working distance, the distance between the objective lens and the object.
Coarse Adjustment Knob
Moves the lens or the stage a relatively great distance and should only be used with the scanning(4x) and the low power(10x) objectives.
Fine Adjustment Knob
Used for precision focusing with all objectives.
Stage
The platform beneath the objective lenses. It supports the slide.
Iris Diaphragm
A circular opening beneath the stage. Controls the amount of light passing through the object.
Light Source
It is built-in and operated by the light switch. It provides the light to form the magnified image.
When magnification is higher illumination is…
Lower.
When magnification is higher the field of view is what?
Lower.
True or False: The movement of the object in a compound microscope is opposite.
True.
True or False: The movement of the object in a dissection microscope coincides.
True.
How do you calculate magnification?
The ocular lens (10x) x the objective lens.
Example: 10 x scanning lens(4x) = 40
What do green plant cells (Elodea plant) contain??
Cell wall, nucleus, central vacuole, cell membrane, cytoplasm, and chloroplasts.
What do non green plant cells (Onion) contain?
Cell wall, nucleus, central vacuole, cytoplasm, and cell membrane.
What do animal cells (Cheek) contain?
Nucleus, cell membrane, cytoplasm.
What do animal cells (Muscle) contain?
Nucleus, cell membrane, cytoplasm.
Identify 3 cellular features present in both plant and animal cells.
Nucleus, cell membrane, and cytoplasm.
What cytoplasmic component is found only in green plants?
Chloroplasts.
What do green plant cells have that animal cells don’t?
Chloroplasts, central vacuole, and a cell wall.
True or False: The central vacuole pushes the nucleus to the side of the cell in plant cells.
True.
What occupies the center of a plant cell?
Central vacuole.
Elodea leaf cell.
The shape is rectangle. It has two layers of cells.
Onion Epidermal Cells.
Plasma membrane is lining the cell wall internally. Estimated size is 2mm. The central vacuole is at the center.
What is mitosis?
Eukaryotic cells divide (cell division), and an orderly process of duplicating and equally dividing the genetic material in the nucleus.
What happens in interphase?
Cell divides, exact duplication of each chromosome forming two identical strands. Starts with 6 chromosomes and ends with 12 chromosomes.
What happens in prophase?
Replicated chromatin threads cool forming chromosomes, nuclear membrane starts to disintegrate, centrioles go to opposite poles of the cell and the spindle is formed, the chromosomes move toward the center of the cell, nuclear membrane is no longer visible.
What happens in metaphase?
Chromosomes align in the middle of the cell. Each duplicated chromosome is composed of 2 chromatids, they are attached to spindle fibers that extend to each centriole.
What happens in anaphase?
Two identical chromatids of each pair separate and move toward opposite poles of the cell.
What happens in telophase?
Reverse of prophase. Chromosomes begin to uncoil and elongate while a new nuclear membrane forms around each set of chromosomes. Elongation of the cell occurs followed by cleavage furrow(a construction of the cell at the center). Marks beginning of cytokinesis.
How many chromatin strands are present as prophase begins?
12 strands.
How many chromatids (coiled chromatin strands) in prophase?
12 chromatids.
How many doubled chromosomes in metaphase?
6 chromosomes.
How many chromosomes in anaphase?
12 chromosomes.
How many chromosomes in each daughter cell?
6 chromosomes.
What is diffusion?
The movement of molecules “down the concentration gradient” (from high to low). Does not require outside energy.