Exam 1 Flashcards
Process of embryonic stem cell isolation
- Frozen blastocysts from fertility clinics
- ICM separated from trophoblast (placenta)
- Cell are disassociated and cultured
What is the ICM?
What day post fertilization do we see pluripotent stem cells?
Cluster of cells that have the potential to become embryos
10-12 days
Sketch diagram on a blastocyst and label ICM
Explain the various potencies of cells in mammals and give an example
- Totipotency - cells have potential to become all things (zygote)
- Pluripotency - cells have the potential to become many cells (embryonic stem cells)
- Multi potency - cells have the potential to become many things but not all (adult stem cells)
- Unipotency - somatic cells (skin cell)
4 common types of stem cells
- Embryonic
- Tissue-specific
- Induced pluripotent
- Mesenchymal
How are ESC different than iPSCs?
ESC are found in the ICM & iPSCs are derived from skin or blood cells
5 sources of Multipotent Stem Cells in the body
- Bone marrow
- Adipose fat
- Placental
- Hair follicle
- Dental pulp
4 areas that stem cells have a positive impact in the future
- Bone marrow for Leukemia
- Nerve cells for parkinsons
- Heart muscle for heart disease
- Toxicity testing
Name 4 reprogramming factors for mice and additional 2 for humans
- Sox2
- Oct4
- cMyc
- Klf4
- Lin28
- Nanog
What is karyotyping? Why is it important?
Visual chromosomes in a sample of cells
Helps identify any genetic abnormalities
3 germ layers and example of each
- Ectoderm - skin cells
- Endoderm - organ cells
- Mesoderm - connective tissue
Why is 3D cell culture important?
it allows for a more similar environment like in vivo
importance: drugs cant penetrate the cell in a 2D state
2 challenges with using stem cells in clinical trials
Hit & miss - potential it wouldnt work
Contamination of viruses, bacteria, fungi, yeast
How do you make a human/monkey chimera?
- Take iPSCs from human and add to monkeys blastocyst (ICM) - both cells are present
- Development happens in vitro
- Human cells give rise to ecto, endo, mesoderm
- Human derived organs in monkey
- Kill monkey to retrieve organs
Sources that one can obtain ESCs
Spontaneous non elective abortions
in-vitro fertilizations