Exam 1 Flashcards
Which medical doctor first used phenol as an antiseptic and disinfectant.
a. Pasteur
b. Snow
c. Semmelweiss
d. Lister
d. Lister
Which of these is Koch’s third postulate?
a. Pathogens should be isolated from artificially infected individuals
b. Pure pathogens given to a health individual should cause disease
c. Pathogens should be found in all sick individuals and no well individuals
d. Pathogens should be isolated from sick individuals
b. Pure pathogens given to a health individual should cause disease
What bacteria structures did Cohn discover?
a. Endospores
b. Inclusion bodies
c. Flagella
d. Nuclei
a. Endospores
Which of Whittaker’s kingdoms contained prokaryotes?
a. Plantae
b. Fungi
c. Monera
d. Archaea
c. Monera
Which of these is smallest in size?
a. Yeast
b. Bacterium
c. Virus
d. Arachaea
c. Virus
What disease was Snow tracking in London when he did his epidemiological work?
a. Cholera
b. Plague
c. Pneumonia
d. Influenze
a. Cholera
Which of these is an acellular microbe?
a. Bacterium
b. Bacteriophage
c. Yeast
d. Archaea
b. Bacteriophage
What type of microbes do bacteriologists study?
a. Prokaryotes
b. Fungi
c. Viruses
d. Algae
a. Prokaryotes
Who used swan necked flasks to disprove spontaneous generation of microbes?
a. Lister
b. Koch
c. Pasteur
d. Snow
c. Pasteur
Which scientist used a simple microscope to first view live bacteria?
a. Pasteur
b. Flemming
c. Hooke
d. Leeuwenhoek
d. Leeuwenhoek
Distinguish which list of structural properties and activities are true for ALL cells.
a. cytoplasmic membrane, ribosomes, metabolism, motility
b. cytoplasmic membrane, ribosomes, growth, differentiation
c. cytoplasmic membrane, ribosomes, growth, communication
d. cytoplasmic membrane, ribosomes, metabolism, evolution
d. cytoplasmic membrane, ribosomes, metabolism, evolution
Which of the following best explains how organisms are currently placed into the three major cell lineages or domains?
a. physical characteristics
b. analysis of ribosomal RNA genes
c. metabolic characteristics
d. analysis of the entire genomic DNA
b. analysis of ribosomal RNA genes
Arrange the following types of microorganisms in the order in which they evolved on Earth.
a. anaerobes, anoxygenic phototrophs, aerobes, oxygenic phototrophs, multicellular organisms
b. anaerobes, multicellular organisms, aerobes
c. aerobes, oxygenic phototrophs, anoxygenic phototrophs, anaerobes, multicellular organisms
d. anaerobes, anoxygenic phototrophs, oxygenic phototrophs, aerobes, multicellular organisms
d. anaerobes, anoxygenic phototrophs, oxygenic phototrophs, aerobes, multicellular organisms
Specify which of the following is a major microbial ecosystem.
a. plants and animals
b. fungi
c. sunlight
d. the atmosphere
a. plants and animals
Identify which of the following situations would be harmful rather than beneficial.
a. nitrogen-fixing bacteria in root nodules
b. Escherichia coli as part of the microbial flora in the human colon
c. microorganisms in the rumen of cattle
d. Escherichia coli in ground meat
d. Escherichia coli in ground meat
Differentiate between industrial microbiology and biotechnology.
a. Industrial microbiology uses naturally occurring microbes to make low-cost products, while biotechnology uses genetically engineered microbes to make high-cost products.
b. Industrial microbiology uses naturally occurring microbes to make low-cost products, while biotechnology uses genetically engineered microbes to make low-cost products.
c. Industrial microbiology uses genetically engineered microbes to make low-cost products, while biotechnology uses genetically engineered microbes to make high-cost products.
d. Industrial microbiology uses naturally occurring microbes to make high-cost products, while biotechnology uses genetically engineered microbes to make low-cost products.
a. Industrial microbiology uses naturally occurring microbes to make low-cost products, while biotechnology uses genetically engineered microbes to make high-cost products.
Hypothesize the most plausible reason why scientists accepted spontaneous generation for so many years.
a. Spontaneous generation was based on many hundreds of different observations. Based on the wealth of data, it must be true.
b. Biology was an experimental science from the beginning, and scientists could not falsify spontaneous generation.
c. Many people believed in life forces that could create life from nonliving matter.
d. Biology was primarily an observational science for many hundreds of years until the acceptance of the scientific method.
d. Biology was primarily an observational science for many hundreds of years until the acceptance of the scientific method.
Which of the following best states the germ theory of disease?
a. Only pure cultures of microorganisms cause disease.
b. Some microorganisms can cause disease.
c. All microorganisms can cause disease.
d. Something in the air caused healthy people to become sick.
b. Some microorganisms can cause disease.
Arrange Koch’s postulates in the correct order.
a. pathogen present in all cases of disease; pathogen grown in pure culture; pathogen able to cause disease in healthy host; pathogen re-isolated
b. pathogen present in all cases of disease; pathogen grown in pure culture; pathogen re-isolated; pathogen able to cause disease in healthy host
c. pathogen grown in pure culture; pathogen present in all cases of disease; pathogen re-isolated; pathogen able to cause disease in healthy host
d. pathogen present in all cases of disease; pathogen able to cause disease in healthy host; pathogen grown in pure culture; pathogen re-isolated
a. pathogen present in all cases of disease; pathogen grown in pure culture; pathogen able to cause disease in healthy host; pathogen re-isolated
Which statement illustrates how the development of enrichment culture techniques by Beijerinck contributed to our knowledge of microbial diversity?
a. Enrichment culture technique allows targeting of specific metabolic groups by using selective nutrients at atmospheric incubation conditions.
b. Enrichment culture technique allows targeting of specific metabolic groups by using common nutrients at atmospheric incubation conditions.
c. Enrichment culture technique allows targeting of specific metabolic groups by using selective nutrients and incubation conditions.
d. Enrichment culture technique allows targeting of specific metabolic groups by using common nutrients but selective incubation conditions.
c. Enrichment culture technique allows targeting of specific metabolic groups by using selective nutrients and incubation conditions.
What type of microbe contains axial filaments?
a. Yeast
b. Acid fast
c. Spirochetes
d. Chlamydia
c. Spirochetes
What is the most abundant macromolecule in a cell?
a. DNA
b. Protein
c. RNA
d. Water
b. Protein
What are bacterial cell walls composed of?
a. Cellulose
b. Chitin
c. Lignin
d. Peptidoglycan
d. Peptidoglycan
What term refers to rod-shaped bacteria in chains?
a. Pallisades
b. Streptococci
c. Staphylobacilli
d. Streptobacillus
d. Streptobacillus
What limits the resolving power of a microscope?
a. The image’s magnification
b. Wavelength of light used
c. Number of lenses used
d. Price of the lenses
b. Wavelength of light used
What type of microbe contains teichoic acid?
a. Gram positive
b. Acid fast
c. Mycoplasma
d. Gram negative
a. Gram positive
What size are prokaryotic ribosomes?
a. 70S
b. 40S
c. 60S
d. 80S
a. 70S
What gets larger as cell size decreases?
a. Surface area
b. Volume
c. Genome size
d. Surface area : volume ratio
d. Surface area : volume ratio
What is volutin an example of?
a. Organelle
b. Vacuole
c. Inclusion body
d. Spore
c. Inclusion body
Which of these microbes does not contain murein?
a. Gram negative
b. Acid fast
c. Gram positive
d. Mycoplasma
d. Mycoplasma
An organism of the genus Staphylococcus is ________, while an organism of the genus Spirochaeta is ________.
a. spherical / coiled
b. rod shaped / coiled
c. spherical / rod shaped
d. coiled / spherical
a. spherical / coiled
The morphology of a cell influences its
a. metabolism.
b. surface-to-volume ratio.
c. motility and surface-to-volume ratio.
d. motility.
c. motility and surface-to-volume ratio.
The terms “run” and “tumble” are generally associated with
a. chemotaxis.
b. eukaryotic cells.
c. clustering of certain rod-shaped bacteria.
d. nutrient transport.
a. chemotaxis.
Using phase contrast microscopy on a wet mount of live cells, you observe motile bacilli moving rapidly and randomly through the field of view, changing directions after a brief tumble and taking off in a different direction. These cells are exhibiting ________ motility.
a. swimming
b. twitching or gliding
c. twitching
d. gliding
a. swimming
Archaeans have archaella that rotate like bacterial flagella though they
a. are longer than bacterial flagella.
b. have greater diameters than bacterial flagella.
c. only rotate in one direction.
d. consist of multiple protein types
d. consist of multiple protein types
In general, most cell inclusions function as energy reserves or as a reservoir of structural building blocks.
a. True
b. False
a. True
Based on the table of attributes given below, which of the following statements are FALSE about the two organisms?
Characteristic Bacterium A Bacterium B Endospore formation yes no Capsule no yes Type IV pili yes no Flagella no no Morphology bacillus bacillus
a. Both bacteria may attach to surfaces.
b. Bacterium B is likely to exhibit motility.
c. Bacterium B likely forms a slime layer better than Bacterium A.
d. Bacterium A is more resistant to heat and ultraviolet light.
b. Bacterium B is likely to exhibit motility.
Bacteria with type IV pili
a. have capsules that promote dehydration.
b. possess tubular or stalk-like extensions of their cells.
c. live in aquatic environments.
d. likely exhibit twitching motility.
d. likely exhibit twitching motility.
Hydrolytic enzymes function in the
a. transport of substrates within the cell.
b. regeneration of the periplasm.
c. chemotactic response, particularly in gram-negative Bacteria.
d. initial degradation of nutrients.
d. initial degradation of nutrients.
The lipopolysaccharide (LPS) layer is found ONLY in the cell walls of
a. gram-positive Bacteria.
b. Eukarya.
c. gram-negative Bacteria.
d. Archaea.
c. gram-negative Bacteria.
Lysozyme is an enzyme that can ultimately lyse and kill eukaryotic cells by breaking β-1, 4-glycosidic bonds in peptidoglycan.
a. True
b. False
b. False
The peptide interbridge crosslinking between peptidoglycan layers is found ONLY in the cell walls of
a. Archaea.
b. gram-positive Bacteria.
c. gram-negative Bacteria.
d. Eukarya.
b. gram-positive Bacteria.
Bacteria stain as gram-positive or gram-negative because of differences in the cell
a. cytoplasm.
b. wall.
c. nucleus.
d. chromosome.
b. wall.
Some membrane proteins are involved in bioenergetic reactions, while others are involved in membrane transport.
a. True
b. False
a. True
The lipids in the cytoplasmic membrane of Bacteria and ________ contain ester linkages, while the cytoplasmic membrane of ________ contain ether linkages.
a. Archaea / fungi
b. Eukarya / Archaea
c. Archaea / Eukarya
d. Eukarya / prokaryotes
b. Eukarya / Archaea
Compared to Eukaryotes, Bacteria and Archaea have ________ surface-to-volume ratios, causing ________ nutrient exchange.
a. lower / lower
b. lower / higher
c. higher / higher
d. higher / lower
c. higher / higher
Small acid-soluble proteins (SASPs) protect DNA from ultraviolet light and are found in high numbers within
a. gram-negative Bacteria.
b. endospores.
c. gram-positive Bacteria.
d. inclusion bodies.
b. endospores.
Some archaea have unique phospholipids in their cytoplasmic membrane that
a. form a monolayer due to the presence of diglycerol tetraethers.
b. form a bilayer due to the presence of phosphatidylethanolamine.
c. form a stable ring structure due to the presence of crenarchaeol.
d. form a bilayer due to the presence of sterols.
a. form a monolayer due to the presence of diglycerol tetraethers.
Eukaryotes have ________ in their cytoplasmic membranes, which serve to strengthen and stabilize the membrane and make it less flexible. Many bacteria have similar molecules, known as ________, in their cytoplasmic membranes that have a similar role.
a. phospholipids / lipopolysaccharides
b. lipids / phospholipids
c. ether bonds / ester bonds
d. sterols / hopanoids
d. sterols / hopanoids
The cell wall of a Gram-positive bacterium is composed of a thick ________ layer.
a. poly-β-hydroxybutryic acid (PHB)
b. peptidoglycan
c. protein
d. lipopolysaccharides (LPS)
b. peptidoglycan
Which group of proteobacteria are most genetically similar to our mitochondria?
a. Gamma
b. Delta
c. Alpha
d. Beta
c. Alpha