Exam 1 Flashcards
Plant variety that has been produced by selective breeding
Cultivar
Individuals that posess the same genotype irrespective of their homo or heterozygous nature
Isogenic line
Particular spot in the genome where a specific gene marker is located
Locus
DNA produced from RNA transcripts
cDNA
Blood serum containing antibodies against specific antigens
Antiserum
Lab method used to detect specific protein molecules from a mixture of proteins
Western blot
Gel technique used to denature proteins and separate on size
Sds-page
Unpurified protein products from lysing cells
Crude extract
Serum extracted prior to immunization
Pre-immune serum
Collection of cloned DNA sequences complimentary to extracted mRNA
cDNA library
Expression vector designed to express the genes on it
Lambda gt ll
Mapping method done by breaking unknown DNA segment into pieces and identifying locations of breakpoints
Restriction mapping
Method used to detect specific RNA molecules among a mixture of RNA
Northern blot
Same/similar relation, relative position, or structure
Homology
Gene hunting technique that traces patterns of disease
Linkage analysis