ex2: chap + fibrous & globular Flashcards
chaperone + fibrous & globular
what happens if hsp70 isn’t present when the temp is high
the hydrophobic regions of the unfolded protein will stick to the hydrophobic regions of other proteins and make a mess, causing aggregation + damage to tissue
what does hsp70 do
binds to the ho region of an unfolded protein to keep it to itself
what are the two parts of hsp60
groes and groel
how does groel cavity work
groel cavity is hydrophobic, this attracts the misfolded protein. groes cap goes on and makes cavity be polar. creates good environment for protein to refold.
3 characteristics about fibrous proteins
insoluble, simple REPEATING linear strands or sheets (secondary structure), and are structural proteins
why are fibrous proteins insoluble
since they are linear, their hydrophobic regions are exposed
all fibrous proteins contain 1 type of repetitive _ structure
secondary
what is the structure of alpha keratin
coiled coil, 2 alpha helix twisted together
what links the structure of alpha keratin
disulfide bonds, they link the helices together
how does collagens structure differ from alpha keratins
collagen forms a triple helix (3 alpha helix’s) while alpha helix uses 2 alpha helix to form a coiled coil
what bonds tighten collagen
covalent + vdw
how many residues per turn does collagen have? what comes after _ residues?
3, gly
benefit of having gly in collagen structure?
small = easier to tighten the structure
what is lysil oxidase
enzyme that modifies lysine. this allows lysine to form covalent cross link bonds to hold the structure together
what is the structure of silk fibroin + bonds that hold it together
b-sheets w vdw = repeating secondary structure
whats special about the ala-gly-ala-gly seq in silk fibroin?
interdigitating of ala and gly allows for more contact in the surface areas of each sheet = more vdw bonds = more stability
where are the hydrophobic regions of globular proteins?
on the inside
what does the structure of globular proteins consist of?
several 2 degree structures = complex 3 degree structure
