Evolution of E. coli

Question 1

What was the Hypothesis?

Answer 1

That there was historical contingency in the evolution of citrate-use.

Question 2

What was the evidence for the hypothesis?

Answer 2

Contingent adaptations should display (1) being rare and (2) time lags … the tendency to produce the beneficial mutation should increase after the underlying mutation(s) happen. Cit+ variants should re-evolve more often in the replays using clones sampled from later generations of the Ara-3 population.