Evaluation of IAT Panel

Question 1

Step 1 Auto Control

Answer 1

Check Auto Control (2 drops patient serum, 1 drop RBCs)
Positive - Auto or Allo Antibodies Present
Negative - Allo Antibodies Present

Question 2

Step 2 Evaluate phases

Answer 2

IS/RT - IgM mostly with some IgG
37 C and AHG - IgG Antibodies

Question 3

Step 3 Evaluate Reaction Strengths

Answer 3

Variable Strengths/Different phases of Reactions
- Multiple Antibodies
- Dosage
- Hemolysis (Anti Le, Anti P)
- mf (Anti Lu, Anti Sd)

Question 4

Step 4 Rule out Antibodies

Answer 4

Can only rule out cells that are Negative in All phases of testing and positive with Check Cells