Eukaryotic Microbes: Protists Flashcards

1
Q

What is the main characteristic feature of Euglenozoans we examined and what are the two groups?

A
  • they are flagellated (characteristic feature is a crystalline rod in flagellum- nobody knows why haha)
  • Two major groups: Kinetoplastids and Euglenids
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2
Q

Euglenozoans:

- Describe Kinetoplastids?

A
  • contain kinetoplasts ( mass of DNA in a single large mitochondrion)
  • most important genus for humans is Trypanosoma
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3
Q

Euglenozoans:

- Describe Euglenids

A
  • Facultatively phototrophic

- lose chloroplast and survive by heterotrophy in the dark

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4
Q

What are the shared characteristics of Diplomonads and Parabasalids?

A
  • they probably have an early branching lineage
  • flagellated and live in anaerobic environments
  • Amitochondriate (lack mitochondria)
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5
Q

Describe the characteristics of Diplomonads

A
  • two nuclei
  • mitosomes (double membrane sacs without electron transport chain)
  • most important for humans = Giardia intestinalis
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6
Q

Describe the characteristics of Parabasalids

A
  • parabasal body(supports golgi) and hydrogenosomes
  • live in body tracts ( GI and GU)
  • most important for us is Trichomonas vaginalis
  • sometimes the trichomonad is buried in cellular debris and only the violent motion of the flagella can be seen
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7
Q

Describe the main characteristic of Alveolates and what are the important groups.

A
  • characterised by cortical alveoli -> f(x) is unknown: osmoregulation, secretion?
  • Three groups: Ciliates, dinoflagellates and apicomplexans
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8
Q

Alveolates:
1. Ciliates
L> Describe this group

A
  • all produce cilia, used for motility and often for feeding (ex Paramecium)
  • have macro and micro nucleus
  • anaerobic fermenters in the gut
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9
Q

Alveolates:
2. Dinoflagellates
L> Describe this group

A
  • two flagella (obvious) which drive whirling motion

- aquatic and can be toxic (red tides) and bioluminescent

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10
Q

Alveolates:
3. Apicomplexans
L> Describe this group

A
  • all are obligate animal parasites
  • contain degenerate chloroplasts called APICOPLASTS - thought to originate from red alga
  • include major human pathogens Plasmodium, Toxoplasma
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11
Q

Explain the main characteristic of the protist group Stramenopiles and what it’s major groups are.

A
  • based on hairy flagella
  • Groups=
    1. Brown Algae (multicellular)
    2. Golden Algae
    3. Diatoms ( silica cell wall)
    4. Oomycetes
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12
Q

Stramenopiles:
- Oomycetes
L> describe this group
*What causes potato blight?

A
  • previously thought to be fungi (via filamentous growth, multinucleate hyphae)
  • BUT BUT BUT
  • cell wall is cellulose
  • they are flagellated
  • diploid phase is DOMINANT
  • Phytophthora infestans causes potato blight
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13
Q

Describe the problems with eukaryotic phylogeny!

A
  • rRNA sequencing gives ancient origin for simple eukaryotes (microsporidia, diplomonads etc)
  • BUT these are secondairly simplified because of the loss of mitochondria
  • THEREFORE the most likely scenario is an early diversification
  • The final phylogeny is therefore unresolved - lol great
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14
Q

Describe Cercozoans and Radiolarians

- unifying feature??

A
  • unifying feature is threadlike PSEUDOPODIAL movement (note that amoebozoa do this too)
  • some are secondarily phototrophic (chlorarachniophytes)
  • but most are heterotrophs that produce a shell called a TEST
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15
Q

Describe Amoebozoa

A
  • use pseudopodial movement but via bigger (lobe like) protrusions
  • diverse group, important (to us) for two reasons:
    L> Pathogens (ex Entamoeba)
    L> as cell biology models (Dictyostelium)
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16
Q

What are the five main protist phyla?

A
  1. Cercozoans
  2. Stramenopiles
  3. Alveolates
  4. Euglenozoa
  5. Amoebozoa