Eukaryotic Microbes Flashcards

1
Q

Prokaryotes versus Eukaryotes

A
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2
Q

Fungal cell

A

Typical eukaryotic cell structure, except: • ergosterol instead of cholesterol in plasma membrane (NB also in some protists) • very rarely flagellated • cell wall

• Composed of chitin • long chain polymer of Nacetylglucosamine • beta1,4 linkages • synthesised by chitin synthase (usually multiple paralogues in a genome)

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3
Q

Fungal lifestyles

A

• Always yeast (Saccharomyces cerevisiae) • Always hyphal (Agaricus bispora) • A mixture (Candida albicans) • Reproduction is often asexual • hyphal spread • single cell budding • asexual spores

Sexual reproduction in fungi • A nightmare!! • Classical mating involves • two mating types (alpha and a, or minus and plus), which can sometimes switch • fusion results in diploid cell that undergoes meiosis to generate haploid recombinants

for many fungi, sexual states are not known • often diploids and haploids are both stable • the morphology of mating is highly variable • mycologists give different names to the two states: • anamorphs (asexual/imperfect) & teleomorphs (sexual/perfect) • these can have different binomials, e.g. • Cryptococcus neoformans (yeast) = Filobasidiella neoformans (hyphal)

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4
Q

Algae

A

Three groups of algae: red, green and brown • brown algae are all multicellular (= seaweed), highly derived species that arose through secondary endosymbiosis • NB: ‘blue-green algae’ are not algae, but bacteria, and correctly known as cyanobacteria

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5
Q

Red Algae

A

• Some unicellular, many multicellular • Often, but not always, red - due to phycoerithrin (an ‘accessory’ pigment) • Typically marine (but some freshwater)

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6
Q

Green algae

A

Some unicellular, many multicellular • Typically freshwater (but some marine) • Evolutionarily very important as: • the ancestor of plants • an insight into the evolution of multicellularity (Volvox

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7
Q

Protists

A

A polyphyletic (and very messy!) group • Loosely defined as “unicellular eukaryotes without a cell wall”, but no shared defining feature • “Protozoa” is sometimes used interchangeably, but sometimes as a subgroup (the most ‘animaly’ protists)

No unifying morphological or biochemical feature for all protists • But within the protists, different groups are frequently characterised by a unique, shared character

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8
Q

Fungal Nucleus

A

Double membrane bound organelle ranging in size from 1-2 µm to 20-25 µm in diameter • Ploidy • most fungi haploid with 6-20 chromosomes • some fungi naturally diploid • Others alternate between haploid and diploid • Unique features of fungal nuclei • Membrane remains intact during mitosis • No clear metaphase plate

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9
Q

Fungal Cytoplasmic Organelles

A

Plasma membrane - phospholipid bilayer • Anchorage for enzymes/proteins such as chitin synthases or glucan synthases • contains ergosterol • Chitosomes • microvesicles for chitin synthesis

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10
Q

The Fungal Cell Wall

A

Structural Barrier • Environmental interface • Protects against osmotic lysis • Contains pigments for protection • Binding site for molecules • Mediates interaction with other organisms

Mannans give fungal cells a detectable signature

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11
Q

Filamentous Fungi

A

Main structure: Hyphae - rigid tubes containing cytoplasm Interconnected compartments, not individual cells

septate hyphae: joint cells have distinct separations called septa, contain pores

coenocytic hyphae: hyphae consists of fused cells, multinucleated

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12
Q

Filamentous Fungi - Growth

A

Mass of hyphae = mycelium (plural: mycelia) Vegetative hyphae • grow along the surface • obtain nutrients Aerial hyphae • stick up from the mycelium • spores for reproduction

Hyphae tips show tropism to a variety of sources: light, nutrients, etc

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13
Q

Yeasts

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• non-filamentous, unicellular, ovaloid • facultative anaerobes • ferment carbohydrates into alcohol and carbon in absence of oxygen

Budding Yeast • Divide by producing outgrowth called bud • Bud enlarges and separates from mother cell Pseudohyphae are not true hyphae: Hyphae: cells tightly attached by shared walls, cytoplasms joined by pores pseudohyphae: daughter cells remain stuck but do not share cytoplasm

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14
Q

Fission Yeast

A

• Division by elongation of mother cell and mitosis • Daughter cells separate by formation of septa in the centre e.g Schizosaccharomyces pombe

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15
Q

Dimorphic Fungi

A

Grow as multicellular hyphal form or unicellular yeast depending on conditions

Several pathogens of humans exhibit dimorphism • Candida albicans • Histoplasma capsulatum • Mucor circinelloides

Dimorphism occurs in response to a variety of environmental factors

  • Histoplasma capsulatum • 25°C = filamentous • 37°C = yeast
  • Candida albicans • human body = filamentous
  • Mucor circinelloides • oxygen = filamentous • hypoxia = yeast
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16
Q

Chytridiomycota

A

Most primitive fungi Approximately 1,000 species Many chytrids are aquatic The exception - motile zoospores and gametes

17
Q

Glomeromycota

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• Approximately 230 species • Terrestial and widespread • Members form arbuscular mycorrhizas (>80 % plants) • Fungus helps plant to capture nutrients such as phosphor, sulfur and nitrogen • Plant provides carbohydrates

18
Q

Mucormycota

A

Approximately 1,000 species Zygosporangium with zygospores Sexual reproduction is NOT common in fungi!!!

19
Q

Ascomycota

A

Approximately 65,000 species Ascus with ascospores

Conidiospore: asexual spore formed at end of hyphae called conidiophore; Conidia: chains of conidiospores on conidiophore

Life Cycle - e.g. Saccharomyces cerevisiae - a and alpha mating type - Mating is controlled by the MAT gene locus which is flanked by two other loci - MATa and MATalpha - a copy of one of the flanking loci is inserted into the MAT locus and determines mating type - MAT switching can occur after each budding

Saccharomyces cerevisiae Schizosaccharomyces pombe Neurospora crassa Candida albicans Aspergillus fumigatus

20
Q

Basidiomycota

A

• > 30,000 species • Basidium with basidospores • Can form basidiocarp (fruiting body)

Agaricus bisporus Boletus edulis Cantharellus cibarius Amanita muscaria Amanita phalloides Cryptococcus neoformans

21
Q

The Oomycetes

A

a protist (diverse group of eukaryotic microorganisms) • distinct lineage • fungal characteristics • heterotroph • morphology (water mold) • but also plant-characteristics • cellulose cell wall • some have chloroplasts

22
Q

Microsporidia

A

Originally thought to be primitive protists • But now known to be close sister group to fungi • A derived group (i.e. they have lost many features during evolution) because… • …they are all obligate parasites of animals

23
Q

Euglenozoans

A

• Flagellated • characteristic feature is a crystalline rod in flagellum (Why? Nobody knows!) • Two major groups: • Kinetoplastids • Euglenids

two groups

  • Kinetoplastids • Contain KINETOPLAST: mass of DNA in a single large mitochondrion • Most important genus for us is Trypanosoma
  • Euglenids • Facultatively phototrophic • Lose chloroplast and survive by heterotrophy in the dark
24
Q

Diplomonads

A

Two nuclei • Mitosomes (double membrane sacs without electron transport chain) • Most important for us is Giardia intestinalis

An early branching lineage (probably!) • Flagellated and live in anaerobic environments

25
Q

Alveolates

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Characterised by cortical alveoli • function unknown: osmoregulation, secretion? • three important groups: • ciliates • dinoflagellates • apicomplexans

26
Q

• Ciliates

A

• all produce cilia, used for motility and often for feeding (e.g. Paramecium) • macro and micro nucleus • anaerobic fermenters in the gut

27
Q

Alveolates

A
  • Dinoflagellates • two flagella, which drive whirling motion • aquatic and can be: • toxic (“red tides”) • bioluminescent
  • Apicomplexans • all are obligate animal parasites • contain degenerate chloroplasts called APICOPLASTS, thought to originate from a red alga [Nair & Striepen, PLoS Biol, 2011] • include major human pathogens Plasmodium, Toxoplasma
28
Q

Stramenopiles

A

Grouped based on ‘hairy’ flagella. • Major groups are: • brown algae (multicellular) • golden algae • diatoms (silica cell wall) • oomycetes…

29
Q

Oomycetes

A

• previously classified as fungi • filamentous growth, multinucleate hyphae • but • cell wall is cellulose • they are flagellated • diploid phase is dominant • Phytophthora infestans, causes potato blight

30
Q

Cercozoans & Radiolarians

A

Unifying feature is threadlike PSEUDOPODIAL movement (but note that amoebozoa do this too!) • some are secondarily phototrophic (chlorarachniophytes) • but most are heterotrophs that produce a shell called a ‘TEST’

31
Q

Amoebozoa

A

• Also use PSEUDOPODIAL movement, but via bigger (lobe-like) protrusions • Diverse group, important (to us!) for two reasons: • as pathogens (e.g. Entamoeba) • as cell biology models (e.g. Dictyostelium)

32
Q

Fungi as infectious agents

A

MYCOSIS

Primary: virulence factors that enable invasion and grow in healthy host Opportunistic: low virulence, cause of infection in a compromised host (e.g. HIV, cancer, steroid therapy, pregnancy, diabetes)

Superficial > Cutaneous > Subcutaneous > Systemic

33
Q

Pathogenesis of Mycoses

A

Infectious agent: spores, hyphal elements and yeasts

Enter body through respiratory, mucous and cutaneous routes - primary pathogens tend to enter via respiratory route

Dermatophytes and Candida sp. part of the human normal flora

Most mycoses not communicable - Exception: Dermatophytes and Candida sp. transmissible

34
Q

Fungal Diseases

A

Fungal diseases (mycoses) are often: • superficial, e.g. athlete’s foot (Trichophyton), dandruff (Malassezia) • opportunistic (secondary), e.g. thrush (Candida), cryptococcosis (Cryptococcus) • But serious primary mycoses exist (e.g. Coccidiodes) and boundaries are blurred (e.g. Cryptococcus gattii)

Most common serious fungal infection is candidiasis (Candida albicans, or related sp.) • Commensal on skin/body tracts • Disseminating infection involves a DIMORPHIC SWITCH - yeast to hyphal

35
Q

Medically important fungi

A

Primary Pathogens - Histoplasma capsulatum - Blastomyces dermatitidis - Coccidioides immitis - Paracoccidioides brasiliensis

Pathogens with intermediate virulence - Dermatophytes

Opportunistic pathogens - Cryptococcus neoformans - Candida albicans - Aspergillus species - Pneumocystis carinii - Mucormycetes

36
Q

Euglenozoal Diseases

A

• Euglenozoa - Crystalline rod in flagellum • Kinetoplastids - mass DNA in single mitochondrion • Trypanosoma

Trypanosomiasis • Parasite is transmitted by invertebrate vector: • Tsetse fly (Glossina sp.) for T. rhodesiense/gambiense • Assassin/kissing bug (Reduviidae) for T. cruzi

37
Q

Amoebozoal Diseases

A

Move via extension of lobe-like pseudopods • Human pathogens include Entamoeba histolytica and Acanthamoeba castellani

Entamoeba histolytica • Causes amoebic dysentery • Cysts ‘hatch’ in the intestine • Penetrate the gut wall, causing bloody diarrhoea

Acanthamoeba spp. Free-living amoebae that also produce cysts • Cause serious infection in immunocompromised hosts • Most common presentation is AMOEBIC KERATITIS

38
Q

Diplomonads & Parabasalids (Diseases)

A

• Both - amitochondriate • Diplomonads - two nuclei and mitosomes • Parabasalids - parabasal body and hydrogenosomes

Usual transmission is faecal-oral, via contaminated water • Due to the formation of resistant CYSTS • Symptoms (explosive diarrhoea etc.) either a) acute, b) chronic or c) asymptomatic carriage • treatment is metronidazole

Trichomonas • Sexually transmitted (NB - no cysts), causes inflammation and discharge • 25-50% of women are infected. Men are carriers (generally asymptomatically). • treatment is metronidazole

39
Q

Alveolate Diseases

A

Include dinoflagellate ‘red tides’ and ciliate parasites, but the big problem are the APICOMPLEXANS • Apicomplexans are ALL obligate parasites • Our selected examples: • Malaria & Toxoplasma

Malaria • Plasmodium species (vivax and falciparum, primarily) • Transmitted by Anopheles mosquitoes • Complex lifecycle with liver stage, blood stage, vector stage

Toxoplasma gondii • Usually asymptomatic, but transmission to foetus is very serious • Humans are accidental hosts