Essential Equations and Calculations

Question 1

What is TBE?

Answer 1

Tris Borate EDTA

Question 2

What is the final concentration of Tris in TBE?

Answer 2

50mM

Question 3

What is the final concentration of Boric Acid in TBE?

Answer 3

44mM

Question 4

What is the final concentration of EDTA in TBE?

Answer 4

1mM

Question 5

What is the final pH of TBE?

Answer 5

8.3

Question 6

What is PBS?

Answer 6

Phosphate buffered saline

Question 7

What is the final concentration of NaCl in PBS?

Answer 7

137mM

Question 8

What is the final concentration of KCl in PBS?

Answer 8

2.7mM

Question 9

What is the final concentration of KCl in PBS?

Answer 9

2.7mM

Question 10

What is the final concentration of Na2HPO4 in PBS?

Answer 10

10mM

Question 11

What is the final concentration of KH2PO4 in PBS?

Answer 11

1.8mM

Question 12

What is the final pH of PBS?

Answer 12

8.3-8.5

Question 13

Dilutions equation

Answer 13

C1V1=C2V2

Question 14

Beer-Lambert equation

Answer 14

A=ecl
A= absorbance no units
e= molar extinction coefficient in m-1cm-1
c= concentration in moles
l= length of the light path in cm

Question 15

buffer solution prep

Answer 15

1. workout final concentrations
2. decide stocks needed
3. available equipment
4. volumes needed
5. calculations to prep target stocks and volumes
6. write protocol

Question 16

Beer-Lambert Law

Answer 16

There is a linear relationship between absorbance and concentration of an absorbing substance which is limited by stray light

Question 17

How do monochromators work?

Answer 17

They reduce stray light by channelling multiple wavelengths of the light source to a single wavelength

Question 18

What is stray light?

Answer 18

Any wavelength outside the band transmitted by the monochromator