Equine clinical pathology, random, passports (notifiable diseases, anaemia, protein, microchips) Flashcards
What equine notifiable diseases are there?
Equine Infectious anaemia (EIA) Equine viral arteritis Rabies Vesicular stomatitis Dourine Epizootic lymphangitis Equine viral encephalomyelitis Glanders West Nile Virus African Horse Sickness Contagious equine metritis
Examples of equine endemic contagious infectious diseases?
Equine herpesviruses 1 and 4 Equine coital exanthema (EHV 3) Strep equi equi Rhodococcus equi Salmonella and Clostridial diarrhoea Equine influenze Etc
Zoonotic infections from horses?
Salmonella enterica CLostridium difficile Crytosporidium parvum Rhodococcus equi MRSA (Rabies)
Normal horse rectal temperature?
36.5 - 38.5C
Define positive and negative predictive values?
PPV = number of positive results that are actually positive NPV = number of negative results that are actually negative
What is the adiponectin test?
= hormone from fat
Concentration can be used for diagnosis of laminitis risk in ponies
What happens with a strangulation obstruction of bowel in horses? How will this be detected by clinical pathology?
- Water and electrolytes move into the bowel
- > High PCV (“haemoconcentrated”) - No blood supply to bowel -> anaerobic metabolism, ischaemic necrosis -> increased permeability of blood vessels -> leakage of blood and protein into peritoneal fluid
- > High lactate in blood/peritoneal fluid (from anaerobic resp)
- > High RBCs and protein in peritoneal fluid - SIRS -> margination off neutrophils, activation of coagulation, low BP -> poor renal perfusion, reduced GFR
- > Increased creatinine and urea
How much blood do horses have?
8-9% BW
500kg horse has about 40-45L
How to get serum and plasma from a blood sample?
Serum: plain tube, allow to clot, centrifuge and take fluid = plasma with the coagulation proteins removed
Plasma: mix with anticoagulant, centrifuge and take fluid
Normal horse PCV?
Hot blooded: 32-48
Cold blooded: 24-44
Role of spleen with PCV?
Huge reserve of RBCs in spleen
At rest, approx 1/3 RBCs are in the spleen
Exercise/stress -> adrenaline -> spleen contracts and releases RBCs into circulation
Can increase PCV from 30-40% to 50-70% at max exercise
Normal blood smear findings in a horse?
Rouleax formation - horses have weaker surface negative charge on RBCs so tend to clump more
Lack of peripheral signs of regeneration (reticulocytes not a reliable sign)
Types and causes of erythrocytosis?
Relative: dehydration or splenic contraction
Absolute primary: polycythemia vera
Absolute secondary: chronic hypoxia
Causes of anaemia in horses?
Acute haemorrhage: - trauma Chronic haemorrhage: - parasites - gastric ulceration - guttural pouch mycosis Intravascular haemolysis: - immune mediated disease - intracellular RBC parasites - plant and chemical poisoning (e.g. red maple leaves, onions, phenothiazine) - bacterial infections (e.g clostridium) Extravascular haemolysis: - fragmentation of RBCs (DIC, vasculitis) - immune mediated disease (EIA virus, penicillin) Primary non-regenerative: - bone marrow disorders Secondary non-regenerative: - chronic disease - lack of erythropoietin, chronic renal failure - iron deficiency
How to tell if red urine is haemoglobinuria or haematuria?
Centrifuge and if Hb stays red???