Equations Flashcards
Henderson Hasselbalch Equation
pH= pKa + log (base/acid)
Keq
([C]^c [D]^d) / ([A]^a[B]^b)
pH=
pH = -log [H+]
At equilibrium delta G=
0
Delta G, Entropy and Enthalpy Equation
Delta G= Delta H - T Delta S
Acid Dissociation Constant Ka
Ka= [H+][Base] / Acid
pKa
pKa= -log Ka
Relates Keq and Delta G not
Delta G not = -RT ln Keq
Relates Keq and Delta G and Delta G not
Delta G= Delta G not + RT ln Keq
How to Calculate Delta S Entropy
Delta S = Kb ln W
Kb= Boltzman constant, W= #of ways to arrange
Myoglobin Fractional Saturation % Y
Y = [Mb O2] / ( [MbO2] + [Mb] ) MbO2 = Oxygen bound to Myoglobin, Mb unbound Myoglobin
Myoglobin Fractional Saturation % Y Pressure in O
Y = pO2 / (pO2 + K)
Michaelis Menton Equation
v = Vmax [S] / ( Km + [S] )
2 unknowns Vmax and Km
Michaelis Menton Equation Competitive Inhibition
v = Vmax [S] / ( Alpha*Km + [S] )
Michaelis Menton Equation UnCompetitive Inhibition
v = Vmax [S] / ( Km + alpha prime [S] )
Line Weaver Burke Equation
1/v = (Km/Vmax) * (1/[S]) + (1/Vmax)
y intercept = 1 / Vmax
x intercept = -1 / Km
Line Weaver Burke Equation Competitive Inhibition
1/ v = (alphaKm/Vmax) * (1/[S]) + (1/Vmax)
Line Weaver Burke Equation Uncompetitive Inhibition
1/v = (Km/ Vmax) * (1/[S]) + (alpha prime/Vmax)
Ki to Calculate the Strength of Enzyme
Competitive Inhibition- Ki = [E][I] / [EI]
Uncompetitive Inhibition Ki prime = [ES] [I] / [ESI]
The smaller the better.
Coefficient of Competitive Inhibition and Noncompetitive Inhibition
alpha= 1 + ([I]/Ki)
alpha prime = 1 + ([I]/Ki prime)
Michaelis Menton Constant
Km = (k-1 + k2) / k1
E + S ES —k2–> E + P
