Enzymes - Michaelis-Menten Kinetics Flashcards
Enzyme Kinetics
This is the study of understanding rate of reaction and how it changes to various experimental parameters
What may complicate enzyme kinetics?
Substrate concentrations changes during reaction in converstion to the product
How can substrate change be determined?
Initial Rate V0
What is the state of the substance at inital rate?
Enzyme is in nanomolar quantities whilst subtrate on 5-6 orders of amgnitude.
How can [S] be limited to few precent?
If only hte begining of the reaction is monitred.
What can inital rate be used to study
Inital rate as a funciton of [S] : at low substrate concentraitons, V0 increases near linearly with increasing [S]
Maximum Velocity
This is where all enzymes at maximum spped at high substrate concentration
How is ES complex formation described?
E + S <–>ES
What are the two steps of a reaction?
E+S,<->ES
ES<–> E+P
What does ES<–>E+P describe?
Complex break down forming enzyme and reaction product
What do ES and E concentration differ depend on?
S concnetratnion, at low most enzyme in E form, where rate of activity is proportional to S as equilibrium favours more ES formaiton with S increase
When does maximum velocity occur?
When al enzymes present are in ES complex formation, where further S increase does not affect rate due to enzyme saturation
What is important to note when P is formed?
E is able to form ES again to catalyse another reaciton.
Pre-Steady State
This occurs when there is a rapid burst of ES complexes forming in the beginning the process is slow because the ES is still forming, then it increases
What does S and V0 relationship approach?
Rectangular hyperbola