Enzymes (Metabolism)

Question 1

What are the 4 things that affect reaction velocity (Vm)?

Answer 1

1. Temperature
2. pH
3. Substrate Concentration
4. Enzyme Concentration

Question 2

What does Km represent?

Answer 2

Affinity of Enzyme for Substrate

Question 3

What are the types of enzyme inhibition and their sub-types?

Answer 3

1. Reversible
   a. Competitive - inhibitor competes with substrate for active sites
   b. non-competitive - inhibitor and substrate bind at different sites. (lowering E efficiency
   c. uncompetitive - binds to active site AFTER substrate does
2. Irreversible

Question 4

What are the main ways of enzyme regulation?

Answer 4

1. Allosteric - can alter affinity for S or maximal activity (positive and negative effectors)
2. Covalent modification - usually by addition or removal of phosphate
3. Enzyme synthesis

Question 5

What does protein kinases do?

Answer 5

With atp it catalyzes (activates) enzymes