ENZYMES LABORATORY Flashcards
WHAT IS THE ENZYME NOMENCLATURE FOR ALP
ALKALINE PHOSPHATASE 3.1.3.1
Major tissues of the ALP
Intestinal, Placenta, Bone, Liver
Another name of ALP
Alkaline Orthophosphoric Monoester Phosphohydrolase
It is involved in the hydrolysis of organic phosphate esters to alcohol and phosphate ion in an ALKALINE pH
ALP
TRUE OR FALSE: BONE ISOENZYMES ARE INCREASED IN CHILDREN AND GERIATRIC
TRUE. THIS IS DUE TO INCREASED OSTEOBLASTIC ACTIVITY
THE LIVER AND BONE ISOENZYMES ARE INHIBITED BY WHAT?
LEVAMISOLE AND UREA
THE PLACENTA AND INTESTINAL ISOENZYMES OF ALP ARE INHIBITED BY WHAT
PHENYLALANINE
WHAT CAUSES INCREASED LIVER ALP
OBSTRUCTIVE JAUNDICE
WHAT CAUSES INCREASED BONE ALP AND THIS HAS THE HIGHEST ELEVATION OF ALP
OSTEITIS DEFORMANS OR PAGET’S DISEASE
PLACENTAL ALP IS SEEN IN WHAT WEEK OF NORMAL PREGNANCY
16-20TH WEEK
INTESTINAL ALP IS INCREASED DUE TO INTAKE OF FATTY MEALS. WHAT BLOOD TYPE CAN THIS BE OBSERVED
B AND O
IN ISOENZYME DIFFERENTIATION FOR ALP, ARRANGE THEM FROM CATHODAL TO ANODAL
INTESTINAL –> PLACENTA –> BONE –> LIVER
AT WHAT TEMPERATURE AND TIME IS HEAT FRACTIONATION TEST FOR ALP ISOENZYME DIFFERENTITATION SET?
56 C FOR 15 MINUTES
ARRANGE THE ISOENZYMES OF ALP FROM STABLE TO LABILE
PLACENTA –> INTESTINE –> LIVER –> BONE
IN UREA INHIBITION TEST FOR ALP ISOENZYME DIFFERENTIATION, WHAT IS INACTIVATED OR DESTROYED WITH THE USE OF 2-3M UREA?
BONE AND LIVER ISOENZYME
WHAT ARE THE MINOR ISOENZYMES OF ALP
REGAN (LUNG) AND NAGAO (PANCREASE)
WHAT INHIBITS REGAN ALP?
PHENULALANINE
WHAT INHIBITS NAGAO?
PHENYLALANINE AND L-LEUCINE
WHAT ISOENZYME IS MOST STABLE THAN THE PLACENTA ALP
REGAN (LUNG)
IT IS A VARIANT OF REGAN ALP
NAGAO (PANCREAS) ALP
IN SPECIMEN CONSIDERATION FOR ALP, ROOM TEMPERATURE CAUSE FALSE DECREASE OF ALP DUE TO WHAT CAUSE?
DUE TO LOSS OF CO2
THIS IS THE REFERENCE METHOD FOR ALP DETERMINATION
BOWER’S AND MCCOMB METHOD
THIS IS THE MODIFICATION OF BOWERS AND MCCOMB BY IFCC
SZASZ METHOD
WHAT IS THE PH AND WAVELENTH OF BOWERS AND MCCOMB METHOD?
PH- 10.15
WAVELENTH: 405NM
WHAT IS THE SUBSTRATE AND ENDPRODUCT OF BOWERS AND MCCOMB METHOD
SUBSTRATE: P-NITROPHENYLPHOSPHATE
PRODUCT: P-NITROPPHENOL YELLOW NITROPHENOXIDE ION
WHAT ARE THE INHIBITORS AND ACTIVATORS FOR ALP?
INHIBITOR: P
ACTIVATOR: Mg Mn
WHAT IS THE ENZYME NOMENCLATURE FOR ACP
3.1.3.2
WHAT IS THE MAJOR TISSUE FOR ACP
PROSTATE
WHAT ARE THE NONPROSTATIC SOURCES FOR ACP?
BONE MARROW, RBC, PLATELETS, LIVER, KIDNEY, SPLEEN, SEMEN
OTHER NAME FOR ACP
ACID ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE
THIS IS INVOLVED IN THE HYDROLYSIS OF ORGANIC PHOSPHATE ESTERS AND PRODUCES ALCOHOL AND PHOSPHATE ION IN AN ACIDIC PH
ACP
VAGINAL WASHING IS EXAMINED FOR SEMINAL FLUID ACP-ACTIVITY AND THIS ENZYMATIC ACTIVITY CAN PERSIST FOR UP TO __ DAYS
4
VAGINAL ACP VALUE THAT INDICATES PRESENCE OF SEMINAL FLUID
> 50 IU/L
IN THE DETERMINATION OF PROSTATIC CARCINOMA: WHAT OTHER TEST IS MORE SENSITIVE THAT IS COMBINED WITH ACP?
PROSTATE SPECIFIC ANTIGEN
INCREASED ACP LEVELS ARE OBSERVED IN WHAT DIASEASES?
OSTEOPOROSIS, MULTIPLE MYELOMA, PAGETS DISEASE, GAUCHERS DISEASE, NIEMANN PICH DISEASE, THROMBOCYTOPENIA
DECREASED ACP
NOT OF CLINICAL SIGNIFICANCE
WHAT DOES HEMOLYSIS DO IN ACP DETERMINATION
FALSE INCREASE
THIS CAUSE FALSE DECREASE OF ACP DETERMINATION
USE OF FLUORIDE ANTICOAGULANTS
SERUM ACP ____ WITHIN 1-2 HOURS IF LEFT AT ____
FALSELY DECREASES : ROOM TEMP
WHAT ARE THE COMMON SUBSTRATES USED IN ALP DETERMINATION?
PARANITROPHENYL PHOSPHATE
THYMOLPHTHALEIN MONOPHOSPHATE
ALPHA-NAPTHYLPHOSPHATE
WHAT ARE THE COMMON TESTS FOR ALP?
ROY AND HILLMAN WHICH USES THYMOLPHTHALEIN MONOPHOSPHATE TO FREE THYMPLPHTHALEIN
RIETZ AND GUIBALT WHICH USES 4-METHYLUMBELLIFERYLONEPHOSPHATE TP FLUORESCENCE
WHAT ANTICOAGULANT, PH, AND WAVELENTH IS USED FOR ROY AND HILLMAN AND
CITRATE BUFFER | PH 6.0 | 410-450NM
THIS ISOENZYME DIFFERENTITATION FOR ACP IS BULKY IN NATURE AND NON-SPECIFIC
CHEMICAL INACTIVATION
THIS ISOENZYME DIFFERENTIATION FOR ACP IS NOT EASILY REPRODUCED
ELECTROPHORESIS
THIS ISOENZYME DIFFERENTITAION FOR ACP IS RECOMMENDED APPROACH FOR ACP DETERMINATION
IMMUNOASSAY
TRAP
RBC ACP
NONTRAP
PROSTATIC ACP
WHAT INHIBITS RBC ACP
COPPER AND FORMALDEHYDE
WHAT INHIBITS PROSTATIC ACP
TARTRATE SINCE IT IS NON-TRAP
FORMULA FOR PROSTATIC ACP
TOTAL ACP - TRAP
WHAT IS THE ENZYME NOMENCALTURE FOR AST
2.6.1.1
MAJOR TISSUES OF AST?
CARDIAC, LIVER, SKELETAL MUSCLE
OTHER NAME FOR AST
SGOT SERUM GLUTAMIX-OXALOACETIC TRANSAMINASE
MAJOR ISOENZYMES FOR AST
CYTOPLASMIC 80% AND MITOCHONDRIAL 20%
WHAT CAUSES AN INCREASE AST
MYOCARDIAL INFARCTION, HEPATOCELLULAR DISCORDER, SKELETAL MUSCLE, CHRONIC LIVER DISORDER, MUSCULAR DYSTROPHY, ACUTE PANCREATITTIS
HIGHEST ELEVATIONS OF AST
ACUTE HEPATITIS
WHAT CAUSES FALSE ELEVATIONS OF AST
HEMOLYSIS (10X ELEVATIONS), LIPEMIC,A ND ECTERIC
WHAT CAUSES DECREASED AST?
UREMIA
WHAT IS THE REFERENCE METHOD FOR DETERINING AST?
KARMEN METHOD WHERE WE DETEMINE THE DECREASE IN ABSORBANCE
WHAT COUPLED ENZYMES ARE USED IN KARMEN METHOD FOR AST?
NADH AND MALATE DEHYDROGENASE
WHAT PH AND WAVELENGTH IS USED FOR KARMEN METHOD
PH 7.5 | 340NM
WHAT OTHER METHODS ARE USED FOR AST DETERMINATION ASIDE FROM KARMEN METHOD
REITMAN AND FRANKEL| DIAZONIUM SALT COUPLING| BABSON METHOD
WHAT IS THE ENZYME NOMENCALTURE FOR ALP
2.6.1.2
MAJOR TISSUE SOURCE FOR ALP
LIVER INTESTINE PLACENTA BONE
WHAT ENZYMES DO NOT CONTAIN ANY ISOENZYMES?
ALP, LIPASE, GGT, 5N’T
OTHER NAME FOR ALP
SGPT SERUM GLUTAMIC PYRUVIC TRANSAMINASE
TRUE OR FALSE: DECREASE IN ALP IS NOT SIGNIFICANT
TRUE
WHAT IS THE REFERENCE METHOD FOR ALP DETERMINATION?
COUPLED ENZYMATIC REACTION
WHAT COUPLED ENZYMES ARE USED FOR THE DETERMINATION OF ALT
NADH AND LD OR LACTATE DEHYDROGENASE
WHAT PH AND WAVELENGTH IS USED FOR COUPLED ENZYMATIC METHOD FOR ALP DETERMINATION
7PH 7.5| 340NM
WHAT OTHER METHOD IS USED FOR ALP DETERMINATION ASIDE FROM COUPLED ENZYMATIC REACTION?
DIAZONIUM SALT COUPLING
WHAT COENZYME IS USED FOR AST AND ALT DETERMINATION
PYROXIDAL PHOSPHATE VITB6
AMINOTRANSFERASES ARE PRESENT IN WHAT SPECIMESNS?
PLASMA, BILE, CSF, SALIVA
CHILDREN: AST__ALT
>
ADULT: AST __ ALT
WHICH HAS A DIURNAL VARIATION AST OR ALT
ALT
WHAT IS THE MAJOR ORGAN AFFECTED FOR AST ? FOR ALT?
AST: HEART
ALT: LIVER
WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?
ALT: ALANINE ALPHA KETOGLUTARIC ACID
AST: ASPARTATE ALPHA KETOGLUTARIC ACID
WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?
AST: GLUTAMIC ACID AND OXALOACETIC ACID
ALT: GLUTAMIC ACID AND PYRUVIC ACID
WHAT COLOR DEVELOPER IS USED FOR AST AND ALT?
2-4 DNPH DINITROPHENYLHYDRAZINE
WHAT COLOR INTENSIFIER IS USED FOR ALT AND AST?
NAOH
BOTH AST AND ALT ARE AFFECTED BY HEMOLYSIS TRUE OR FALSE?
TRUE
BOTH AST AND ALT ARE ELEVATED IN HEPARIN THERAPHY 3X
TRUE
AT WHAT DISEASE DO BOTH ALT AND AST HAVE ARE DECREASED?
RENAL DISEASE
WHAT RATIO IS USED FOR AST AND ALT
DE RITIS RATIO
WHAT CAUSES 20X INCREASE OF THE DE RITIS RATIO?
VIRAL AND TOXIC HEPATITIS
WHAT DISEASE IS ASSOCIATED WITH HIGHEST ELEVATION OF DERITIS RATIO
ACUTE HEPATITIS
WHAT DISEASE IS ASSOCIATED WITH MODERATE ELEVATIONS OF DE RITIS RATIO
CHRONIC HEPATITIS, HEPATIC CARCINOMA, IM
WHAT DISEASE IS ASSOCIATED WITH SLIGHT ELEVATIONS OF DE RITIS RATIO?
HEPATIC CIRRHOSIS, ALCOHOL HEPATITIS, AND OBSTRUCTIVE JAUNDICE
> 2 DE RITIS RATIO
ALCOHOLIC HEPATITIS AND HEPATOCELLULAR DISORDER
1-2 DERITIS RATIO
ACUTE HEPATITIS AND CIRRHOSIS
<1 DERITIS RATIO
VIRAL HEPATITIS
WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE
3.2.1.1
MAJOR TISSUE SOURCES OF AMYLASE
PANCREAS AND SALIVARY CELLS OR GLANDS
WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE
ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE
WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE
ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE
OTHER NAME FOR AMYLASE
4-1 GLUCAN-4-GLUCOHYDROLASE
IN AMYLASE, PHYSIOLOGIC DIGESTION OF __ AND __ IS OBSERVED
STARCH AND GLYCOGEN
THIS IS CONSIDERED AS THE SMALLEST ENZYME
AMYLASE
TRUE OF FALSE: AMYLASE IS 15% HIGHER IN FEMALES
TRUE
THIS IS THE MOST PREDOMINANT PANCREATIC AMYLASE ISOENZYME IN THE ONSET OF ACUTE PANCREATITIS
P3 ISOENZYME
WHAT IS THE OTHER NAME FOR AMS SALIVARY TYPE
PTYALIN
WHERE DOES PTYALIN MIGRATE IN ELECTRODE
ANODAL
PATHOLOGIC INCREASE OF THE SALIVARY AMYLASE
PAROTITIS
OTHER NAME FOR THE PANCREATI AMYLASE
AMYLOPSIN
MIGRATION OF PANCREATIC AMYLASE IN THE ELECTRODE
CATHODAL
PATHOLOGICAL INCREASE OF PANCRATIC AMYLASE
PANCREATITIS
WHAT IS ADDED TO THE SPECIMEN TO AVOID THE INACTIVATION OF THE AMYLASE?
DILUTION WITH NACL
WHAT ENDOGENOUS INHIBITORS ARE FOUND IN THE SERUM THAT INHIBIT AMYLASE
WHEAT GERM LECTIN AND TAG
WHAT ANTICOAGULANTS INTERFERE WITH AMYLASE
CITRATE, EDTA, OXALATE
WHAT SUBSTRATE IS USED IN AMYLASE DETERMINATION
STARCH
this test for amylase measures the reducing sugar produced form hydrolyzed starch
SACCHAROGENIC
THIS TEST FOR AMYLASE MEASURES THE DRECREASE IN SUBSTRATE CONNCENTRATION WHERE THE TIME OF DECOLORIZATION = THE AMOUNT OF AMYLASE ANCITVITY
AMYLOCLASTIC
THIS TEST FOR AMYLASE MEASURES THE INCREASE IN COLOR INTENSITY WHERE THE AMOUNT OF STARCH DYE DESTROTYED = INTENSITY OF COLOR = AMOUNT OF AMYLASE ACTIVITY
CHROMOGENIC
THIS METHOD OF AMYLASE DETERMINATION USES A-GLUCOSIDASE AND HEXOKINASE
COUPLED ENZYME
THERE ARE 6 BANDS FORMED IN THE ELECTROPHORESIS OF AMYLASE: WHAT ARE THE FIRST 3 BANDS AND THE LAST 3 BANDS?
FIRST: PTYALIN
LAST: AMYLOPSIN
THE INHIBITION TEST FOR AMYLASE USES TRITICUM VULGARIS AND IT INHIBITS WHAT TYPE OF AMYLASE
S TYPE OR SALIVARY TYPE OR PTYALIN
WHAT IS THE FORMULA FOR AMYLASE CREATININE RATIO:
URINE AMYLASE/ SERUM AMYASE X SERUM CREATININE/ URINE CREATININE
WHAT IS THE NORMAL REF RANGE FOR AMYLASE CREATININE RATIO?
1-5%
INCREASED AMYLASE IN BLOOD AND URINARY SECRETIONS INDICATE WHAT DISEASE. AC RATIO IS 4-15%
ACUTE PANCREATITIS
WHAT DISEASE CAUSES ELEVATED AMYLASE IN THE SERUM AND NORMAL AMYLASE URINARY SECRETION? THIS IS CHARACTERIZED BY AMYLASE BOUNDED TO AN ANTIBODY
MACROAMYLASEMIA
WHAT IS THE ENZYMATIC NOMENCLATURE FOR LIPASE
3.1.1.3
MAJOR TISSUE SOURCE OF LIPASE
PANCREAS
ANOTHER NAME FOR LIPASE
TRIAGLYCEROL ACYLHYDROLASE
IT INVOLVES THE HYDROLYSIS OF FATS TO PRODUCE ALCOHOL AND FATTY ACIDS
LIPASE
THIS IS THE SUPERIOR DIAGNOSTIC TOOL FOR PANCREATITIS
LIPASE
THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE
ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS
THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE
ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS
DECREASED LIPASE VALUES ARE OBSERVED IN
PANCREATIC INSUFFICIENCY
WHAT SUBSTRATE IS USED FOR LIPASE MEASUREMENT
OLIVE OIL
WHAT IS AN ALTERNATIVE FOR THE SUBSTRATE IN LIPASE MEASUREMENT
TIOLEIN OIL
THIS INCREASES THE SENSITIVITY AND SPECIFICITY OF LIPASE MEASUREMENT
BILE SALTS AND COLIPASE
THIS PREVENTS LIPASE INACTIVAITON
THIS PREVENTS LIPASE INACTIVATION
COLIPASE
INHIBITOR FOR LIPASE AND WHICH CAUSES FALSE DECREASE OF LIPASE
HEMGLOBIN (HEMOLYSIS)
BACTERIAL CONTAMINATION CAUSES __ IN LIPASE VALUES
FALSE INCREASE
WHAT IS THE REFERENCE METHOD FOR LIPASE DETERMINATION
CHERRY CRANDAL METHOD
THIS USES OLIVE OIL IN THE DETERMINATION OF LIPASE. INCUBATIION IS DONE FOR 24 HOURS AT 37 C
CHERRY CRANDAL METHOD
THIS IS COMMONLY USED METHOD FOR LIPASE MEASUREMENT; IT IS A COLORIMETRIC METHOD THAT USES MINIMAL SAMPLE
peroxidase coupling
THIS LIPASE MEASUREMENT USES BOTH OLIVE AND TIROLEIN OIL
TURBIDIMETRY
TRUE OR FALSE: DECREASE IN TURBIDIMETRY INDICATE DECREASE IN LIPASE?
FALSE. DECREASE IN TURBIDIMETRY = INCREASE LIPASE
WHAT IS THE ENZYMATIC NOMENCLATURE FOR LACTATE DEHYDROGENASE?
1.1.1.27
THIS ENZYME CAUSES THE INTERCONVERSION OF LACTIC AND PYRUVIC ACID
LACTATE DEHYDROGENASE
WHAT COENZYME IS USED IN LACTATE DEHYDROGENASE?
NAD NICOTINAMIDE ADENINE DINUCLEOTIDE
WHAT ARE THE 2 FORMS OF LACTATE DEHYDROGENASE
H AND M FORMS
WHAT ARE THE MAJOR ISOENZYMES OF LACTATE DEHYDROGENASE
LD1, 2, 3, 4, 5
WHAT IS THE MINOR ISOENZYME OF LACTATE DEHYDROGENASE
LD6
WHAT LD ISOEZNYME HAS THE HIGHEST PERCENTAGE
LD2
what are the sources of LD1 and LD2 clue: RK heart
RBC KIDNEY AND HEART
WHAT ISOENZYME OF LD IS THE MOST ANODAL AND HEAT STABLE
LD 1 AND 2
WHAT ARE THE SOURCES OF LD 2 (CLUE PLS)
PANCREAS LUNGS SPLEEN
THE IS THE INTERMEDIARY ISOENZYME. MODERATE IN ABUNDANCE
LD3
WHAT ARE THE SOURCES OF LD 4 AND 5 (CLUE SSmILI)
SKIN, SMALL, ILIUM, LIVER, INSTESTINE
THIS IS THE LEAST ANODAL AND AND HEAT LABILE AND ARE SCARCE
LD 4 AND LD 5
WHAT IS ANOTHER NAME FOR LD 6
ALCOHOL DEHYDROGENASE
AT WHAT BAND DOES THE LD6 APPEAR IN ELECTROPHORESIS
6TH BAND
THIS IS OBSERVED WHEN THERE IS HIGHEST ELEVATIONS OF LACTATE DEHYDROGENASE
PERNICIOUS ANEMIA
NORMAL ELECTROPHORETIC PATTERN OF SERUM LD AS WELL AS WHEN THERE IS SEIZURE
2>1>3>4>5
CSF AND ACUTE MYOCARDIAL INFARCTION LD ELECTROPHORETIC PATTERN
1>2>3>4>5
LD ELETROPHORETIC PATTERN FOR BACTERIAL MENINGITIS
5>4>3>2>1
WHAT LD ISOENZYMES ARE CONSIDERED AS CANCER MARKERS.
LD 2 3 4
WHAT LD IS ASSOCIATED WTH HEPATIC CARCINOMA ANND TOXIC HEPATITIS
ld 5
THIS ENZYME IS SEVERELY AFFECTED BY HEMOLYSIS
LD LACTATE DEHYDROGENASE
LD LEVEL (>/
>
TRUE OR FALSE: THERE IS DECREASE VALUE OF LD IF SAMPLE IS FROZEN
TRUE. THE STORAGE FOR LD IS AT ROOM TEMP 20 TO 26 C
THIS METHOD FOR LD CONVERTS LACTATE TO PYRUVATE AT PH 8.8 AND THIS IDENTIFIES LD 1
WACKER METHOD
THIS LD METHOD CONVERTS PYRUVATE TO LACTATE AT PH 7.2 AND IDENTIFIES LD 5
WROBLEWSKI LADUE (REVERSE METHOD
IN HEAT DENATURATION FOR LD MEHTODS WHAT ISOENZYME IS STABLE
LD 1
WHAT IS THE ISOENZYME NOMENCLATURE FOR CREATINE KINASE
2.7.3.2
MAJOR TISSUE SOURCES FOR CREATINE KINASE
CARDIAC MUSCLE, SMOOTH MUSCLE, SKELETAL MUSCLE AND BRAIN
TRUE OR FALSE: ATHLETES HAVE INCREASE BASELINE LEVEL OF CREATINE KINASE
TRUE
THIS CAUSES INCREASE OF CK LEVELS FOR UPTO 5 TIMES
INTRAMUSCULAR INJECTION
TRUE OR FALSE: BEDRIDDEN PATIENTS HAVE DECREASED CK VALUES
TRUE
THIS IS A MINOR ISOENZYME OF CK SEEN IN ADVANCED MALIGNANCIES
MITOCHONDRIAL CK
WHAT ARE THE DIFFERENT ISOZENZYMES OF CK
CK-BB CK-MB AND CK-MM
THE MOST ANDODAL AND MOST LABILE CK ISOENZYME
CK BB
HOW MANY PERCENT OF CK-MB IS PRESENT IN CARDIAC TISSUE?
WHAT PERCENT OF TOTAL CK INDICATES MYOCARDIAL DAMAGE
20% OF CK MBB IS PRESENT IN CARDIAC TISSUE
>6% OF TOTAL CK = MYOCARDIAL DAMAGAE
THIS IS THE MAJOR ISOENZYME OF CK IN THE SERUM
CK-MM
THIS IS THE LEAST ANODAL CK ISOENZYME
CK-MM
HIGHEST ELEVATIONS OF CK IS SEEN IN THIS DISEASE
DUCHENE DISORDER
PERCENTAGE OF TOTAL CK ATTRIBUTED TO CK-MB
POSSIBLE RELEASE OF CK-MB FROM NON-CARDIAC TISSUE
CK RELATIVE INDEX
CK ACTIVATOR
N-ACETYL-CYSTEINE AND MG
WHAT SUBSTANCE IS FOUND IN THE RBC CAUSING INCREASED CK
ADENYLATE KINASE
INHIBITOR OF CK
URATE AND CYSTEINE
TRUE OR FALSE: CK IS PHOTOSENSITIVE
TRUE
CK WOULD BE DECREASED DURING STORAGE, WHAT REAGENTS ARE USED TO REMEDY THIS
CLELAND’S REAGENT AND GLUTATHIONE
THIS IS THE FORWARD METHOD IN DETECTING CK. WHAT PH AND WAVELENGTH IS USED?
TANZER-GILOVARD METHOD | pH 9 | 340 nm
THIS IS THE INDIRECT METHOD IN MEASURING CK. WHAT PH AND WAVELENTH IS USED?
OLIVER-ROSALSKI METHOD
PH6.8
340NM
WHAT IS THE ENZYME NOMENCLATURE FOR GGT
2.3.2.1
THIS ENZYME IS AN INDICATOR FOR ALCOHOLISM
GGT
HIGHEST ELEVATIONS OF GGT
PRIMARY BILIARY CIRRHOSIS
WHAT METHOD IS USED FOR GGT DETERMINATION AND WHAT WAVELENGTH IS USED?
SZASZ ASSAY
405-410 nm
WHAT IS THE ENZYME NOMENCLATURE FOR TRUE AND PSEUDO CHOLINESTERASE
- 1.1.7 TRUE CHOLINESTERASE
3. 1.1.8 PSEUDOCHOLINESTERASE
WHAT IS ANOTHER NAME FOR TRUE CHOLINESTERASE
ACYLCHOLINE ACYLHYDROLASE
WHAT IS ANOTHER NAEM FOR PSEUDOCHOLINESTERASE
ACETYLCHOLINE ACETYLHYDROLASE
THIS ENZYME IS USED IN THE ASSESSMENT OF PARENCHYMAL FUNCTION
CHOLINESTERASE
INHIBITOR FOR CHOLINESTERASE
SUCCINYLCHOLINE
WHAT IS THE MOST USED METHOD IN CHOLINESTERASE? WHAT WAVELENGTH IS USED?
ELLMAN
410 NM
THIS METHOD IN CHOLINESTERASE DETERMINATION MAKE USE OF A SPECIALIZED EQUIPMENT
MICHEL
WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE
4.1.2.13
WHAT IS OTHER NAME FOR ALDOLASE
FRUCTOSE 1-6 DIPHOSPHATE ALDOLASE
ISOENZYMES OF ALDOLASE AND WHERE ARE THEY FOUND?
ALDOLASE A: SKELETAL MUSCLE
ALDOLASE B : WBC, LIVER, KIDNEY
ALDOLASE C: BRAIN TISSUE
WHAT IS THE MAIN TISSUE SOURCE OF OCT
LIVER
WHAT METHOD IS USED FOR OCT
REICHARD AND REICHARD
WHAT IS THE ENZYMATIC NOMENCLATURE FOR OCT
2.1.1.3
WHAT ARE THE MAIN SOURCES OF LEUCINE AMINOPEPTIDASE
BUS
BILE URINE SERUM
WHAT IS THE METHOD FOR LEUCINE AMINOPEPTIDASE
GOLDBERG AND RUTENBERG
WHAT ARE THE LABORATORY METHODS FOR 5NT
DIXON AND PURDON
BELLFIELD AND GLODBERG
CAMPBELL
ACE NOMENCLATURE
3.4.15.1
MAIN SOURCE OF ACE
MACROPHATGES AND EPITHELOID CELLS
THIS ENZYME IS INCREASED IN ALZHEIMERS DISEASE IN VIA CSF ANALYSIS
ACE
CERULOPLASMIN NOMENCLATURE
1.16.3.1
ENZYME THAT IS A KNOWN MARKER FOR WILSONS DISEASE
CERULOPLASMIN
THIS ENZYME MAINTAINS THE NADPH IN A REDUCED FROM INSIDE THE RBC
G6PD
G6PD NOMENCLATURE
1.1.1.49
