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CLINICAL CHEMISTRY > ENZYMES LABORATORY > Flashcards

ENZYMES LABORATORY Flashcards

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1
Q

WHAT IS THE ENZYME NOMENCLATURE FOR ALP

A

ALKALINE PHOSPHATASE 3.1.3.1

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2
Q

Major tissues of the ALP

A

Intestinal, Placenta, Bone, Liver

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3
Q

Another name of ALP

A

Alkaline Orthophosphoric Monoester Phosphohydrolase

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4
Q

It is involved in the hydrolysis of organic phosphate esters to alcohol and phosphate ion in an ALKALINE pH

A

ALP

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5
Q

TRUE OR FALSE: BONE ISOENZYMES ARE INCREASED IN CHILDREN AND GERIATRIC

A

TRUE. THIS IS DUE TO INCREASED OSTEOBLASTIC ACTIVITY

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6
Q

THE LIVER AND BONE ISOENZYMES ARE INHIBITED BY WHAT?

A

LEVAMISOLE AND UREA

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7
Q

THE PLACENTA AND INTESTINAL ISOENZYMES OF ALP ARE INHIBITED BY WHAT

A

PHENYLALANINE

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8
Q

WHAT CAUSES INCREASED LIVER ALP

A

OBSTRUCTIVE JAUNDICE

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9
Q

WHAT CAUSES INCREASED BONE ALP AND THIS HAS THE HIGHEST ELEVATION OF ALP

A

OSTEITIS DEFORMANS OR PAGET’S DISEASE

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10
Q

PLACENTAL ALP IS SEEN IN WHAT WEEK OF NORMAL PREGNANCY

A

16-20TH WEEK

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11
Q

INTESTINAL ALP IS INCREASED DUE TO INTAKE OF FATTY MEALS. WHAT BLOOD TYPE CAN THIS BE OBSERVED

A

B AND O

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12
Q

IN ISOENZYME DIFFERENTIATION FOR ALP, ARRANGE THEM FROM CATHODAL TO ANODAL

A

INTESTINAL –> PLACENTA –> BONE –> LIVER

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13
Q

AT WHAT TEMPERATURE AND TIME IS HEAT FRACTIONATION TEST FOR ALP ISOENZYME DIFFERENTITATION SET?

A

56 C FOR 15 MINUTES

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14
Q

ARRANGE THE ISOENZYMES OF ALP FROM STABLE TO LABILE

A

PLACENTA –> INTESTINE –> LIVER –> BONE

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15
Q

IN UREA INHIBITION TEST FOR ALP ISOENZYME DIFFERENTIATION, WHAT IS INACTIVATED OR DESTROYED WITH THE USE OF 2-3M UREA?

A

BONE AND LIVER ISOENZYME

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16
Q

WHAT ARE THE MINOR ISOENZYMES OF ALP

A

REGAN (LUNG) AND NAGAO (PANCREASE)

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17
Q

WHAT INHIBITS REGAN ALP?

A

PHENULALANINE

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18
Q

WHAT INHIBITS NAGAO?

A

PHENYLALANINE AND L-LEUCINE

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19
Q

WHAT ISOENZYME IS MOST STABLE THAN THE PLACENTA ALP

A

REGAN (LUNG)

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20
Q

IT IS A VARIANT OF REGAN ALP

A

NAGAO (PANCREAS) ALP

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21
Q

IN SPECIMEN CONSIDERATION FOR ALP, ROOM TEMPERATURE CAUSE FALSE DECREASE OF ALP DUE TO WHAT CAUSE?

A

DUE TO LOSS OF CO2

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22
Q

THIS IS THE REFERENCE METHOD FOR ALP DETERMINATION

A

BOWER’S AND MCCOMB METHOD

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23
Q

THIS IS THE MODIFICATION OF BOWERS AND MCCOMB BY IFCC

A

SZASZ METHOD

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24
Q

WHAT IS THE PH AND WAVELENTH OF BOWERS AND MCCOMB METHOD?

A

PH- 10.15

WAVELENTH: 405NM

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25
Q

WHAT IS THE SUBSTRATE AND ENDPRODUCT OF BOWERS AND MCCOMB METHOD

A

SUBSTRATE: P-NITROPHENYLPHOSPHATE
PRODUCT: P-NITROPPHENOL YELLOW NITROPHENOXIDE ION

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26
Q

WHAT ARE THE INHIBITORS AND ACTIVATORS FOR ALP?

A

INHIBITOR: P
ACTIVATOR: Mg Mn

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27
Q

WHAT IS THE ENZYME NOMENCLATURE FOR ACP

A

3.1.3.2

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28
Q

WHAT IS THE MAJOR TISSUE FOR ACP

A

PROSTATE

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29
Q

WHAT ARE THE NONPROSTATIC SOURCES FOR ACP?

A

BONE MARROW, RBC, PLATELETS, LIVER, KIDNEY, SPLEEN, SEMEN

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30
Q

OTHER NAME FOR ACP

A

ACID ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE

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31
Q

THIS IS INVOLVED IN THE HYDROLYSIS OF ORGANIC PHOSPHATE ESTERS AND PRODUCES ALCOHOL AND PHOSPHATE ION IN AN ACIDIC PH

A

ACP

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32
Q

VAGINAL WASHING IS EXAMINED FOR SEMINAL FLUID ACP-ACTIVITY AND THIS ENZYMATIC ACTIVITY CAN PERSIST FOR UP TO __ DAYS

A

4

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33
Q

VAGINAL ACP VALUE THAT INDICATES PRESENCE OF SEMINAL FLUID

A

> 50 IU/L

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34
Q

IN THE DETERMINATION OF PROSTATIC CARCINOMA: WHAT OTHER TEST IS MORE SENSITIVE THAT IS COMBINED WITH ACP?

A

PROSTATE SPECIFIC ANTIGEN

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35
Q

INCREASED ACP LEVELS ARE OBSERVED IN WHAT DIASEASES?

A

OSTEOPOROSIS, MULTIPLE MYELOMA, PAGETS DISEASE, GAUCHERS DISEASE, NIEMANN PICH DISEASE, THROMBOCYTOPENIA

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36
Q

DECREASED ACP

A

NOT OF CLINICAL SIGNIFICANCE

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37
Q

WHAT DOES HEMOLYSIS DO IN ACP DETERMINATION

A

FALSE INCREASE

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38
Q

THIS CAUSE FALSE DECREASE OF ACP DETERMINATION

A

USE OF FLUORIDE ANTICOAGULANTS

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39
Q

SERUM ACP ____ WITHIN 1-2 HOURS IF LEFT AT ____

A

FALSELY DECREASES : ROOM TEMP

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40
Q

WHAT ARE THE COMMON SUBSTRATES USED IN ALP DETERMINATION?

A

PARANITROPHENYL PHOSPHATE
THYMOLPHTHALEIN MONOPHOSPHATE
ALPHA-NAPTHYLPHOSPHATE

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41
Q

WHAT ARE THE COMMON TESTS FOR ALP?

A

ROY AND HILLMAN WHICH USES THYMOLPHTHALEIN MONOPHOSPHATE TO FREE THYMPLPHTHALEIN
RIETZ AND GUIBALT WHICH USES 4-METHYLUMBELLIFERYLONEPHOSPHATE TP FLUORESCENCE

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42
Q

WHAT ANTICOAGULANT, PH, AND WAVELENTH IS USED FOR ROY AND HILLMAN AND

A

CITRATE BUFFER | PH 6.0 | 410-450NM

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43
Q

THIS ISOENZYME DIFFERENTITATION FOR ACP IS BULKY IN NATURE AND NON-SPECIFIC

A

CHEMICAL INACTIVATION

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44
Q

THIS ISOENZYME DIFFERENTIATION FOR ACP IS NOT EASILY REPRODUCED

A

ELECTROPHORESIS

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45
Q

THIS ISOENZYME DIFFERENTITAION FOR ACP IS RECOMMENDED APPROACH FOR ACP DETERMINATION

A

IMMUNOASSAY

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46
Q

TRAP

A

RBC ACP

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47
Q

NONTRAP

A

PROSTATIC ACP

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48
Q

WHAT INHIBITS RBC ACP

A

COPPER AND FORMALDEHYDE

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49
Q

WHAT INHIBITS PROSTATIC ACP

A

TARTRATE SINCE IT IS NON-TRAP

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50
Q

FORMULA FOR PROSTATIC ACP

A

TOTAL ACP - TRAP

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51
Q

WHAT IS THE ENZYME NOMENCALTURE FOR AST

A

2.6.1.1

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52
Q

MAJOR TISSUES OF AST?

A

CARDIAC, LIVER, SKELETAL MUSCLE

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53
Q

OTHER NAME FOR AST

A

SGOT SERUM GLUTAMIX-OXALOACETIC TRANSAMINASE

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54
Q

MAJOR ISOENZYMES FOR AST

A

CYTOPLASMIC 80% AND MITOCHONDRIAL 20%

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55
Q

WHAT CAUSES AN INCREASE AST

A

MYOCARDIAL INFARCTION, HEPATOCELLULAR DISCORDER, SKELETAL MUSCLE, CHRONIC LIVER DISORDER, MUSCULAR DYSTROPHY, ACUTE PANCREATITTIS

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56
Q

HIGHEST ELEVATIONS OF AST

A

ACUTE HEPATITIS

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57
Q

WHAT CAUSES FALSE ELEVATIONS OF AST

A

HEMOLYSIS (10X ELEVATIONS), LIPEMIC,A ND ECTERIC

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58
Q

WHAT CAUSES DECREASED AST?

A

UREMIA

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59
Q

WHAT IS THE REFERENCE METHOD FOR DETERINING AST?

A

KARMEN METHOD WHERE WE DETEMINE THE DECREASE IN ABSORBANCE

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60
Q

WHAT COUPLED ENZYMES ARE USED IN KARMEN METHOD FOR AST?

A

NADH AND MALATE DEHYDROGENASE

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61
Q

WHAT PH AND WAVELENGTH IS USED FOR KARMEN METHOD

A

PH 7.5 | 340NM

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62
Q

WHAT OTHER METHODS ARE USED FOR AST DETERMINATION ASIDE FROM KARMEN METHOD

A

REITMAN AND FRANKEL| DIAZONIUM SALT COUPLING| BABSON METHOD

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63
Q

WHAT IS THE ENZYME NOMENCALTURE FOR ALP

A

2.6.1.2

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64
Q

MAJOR TISSUE SOURCE FOR ALP

A

LIVER INTESTINE PLACENTA BONE

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65
Q

WHAT ENZYMES DO NOT CONTAIN ANY ISOENZYMES?

A

ALP, LIPASE, GGT, 5N’T

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66
Q

OTHER NAME FOR ALP

A

SGPT SERUM GLUTAMIC PYRUVIC TRANSAMINASE

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67
Q

TRUE OR FALSE: DECREASE IN ALP IS NOT SIGNIFICANT

A

TRUE

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68
Q

WHAT IS THE REFERENCE METHOD FOR ALP DETERMINATION?

A

COUPLED ENZYMATIC REACTION

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69
Q

WHAT COUPLED ENZYMES ARE USED FOR THE DETERMINATION OF ALT

A

NADH AND LD OR LACTATE DEHYDROGENASE

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70
Q

WHAT PH AND WAVELENGTH IS USED FOR COUPLED ENZYMATIC METHOD FOR ALP DETERMINATION

A

7PH 7.5| 340NM

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71
Q

WHAT OTHER METHOD IS USED FOR ALP DETERMINATION ASIDE FROM COUPLED ENZYMATIC REACTION?

A

DIAZONIUM SALT COUPLING

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72
Q

WHAT COENZYME IS USED FOR AST AND ALT DETERMINATION

A

PYROXIDAL PHOSPHATE VITB6

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73
Q

AMINOTRANSFERASES ARE PRESENT IN WHAT SPECIMESNS?

A

PLASMA, BILE, CSF, SALIVA

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74
Q

CHILDREN: AST__ALT

A

>

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75
Q

ADULT: AST __ ALT

A
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76
Q

WHICH HAS A DIURNAL VARIATION AST OR ALT

A

ALT

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77
Q

WHAT IS THE MAJOR ORGAN AFFECTED FOR AST ? FOR ALT?

A

AST: HEART
ALT: LIVER

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78
Q

WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?

A

ALT: ALANINE ALPHA KETOGLUTARIC ACID
AST: ASPARTATE ALPHA KETOGLUTARIC ACID

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79
Q

WHAT ARE THE ENDPRODUCTS FOR AST? FOR ALT?

A

AST: GLUTAMIC ACID AND OXALOACETIC ACID
ALT: GLUTAMIC ACID AND PYRUVIC ACID

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80
Q

WHAT COLOR DEVELOPER IS USED FOR AST AND ALT?

A

2-4 DNPH DINITROPHENYLHYDRAZINE

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81
Q

WHAT COLOR INTENSIFIER IS USED FOR ALT AND AST?

A

NAOH

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82
Q

BOTH AST AND ALT ARE AFFECTED BY HEMOLYSIS TRUE OR FALSE?

A

TRUE

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83
Q

BOTH AST AND ALT ARE ELEVATED IN HEPARIN THERAPHY 3X

A

TRUE

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84
Q

AT WHAT DISEASE DO BOTH ALT AND AST HAVE ARE DECREASED?

A

RENAL DISEASE

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85
Q

WHAT RATIO IS USED FOR AST AND ALT

A

DE RITIS RATIO

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86
Q

WHAT CAUSES 20X INCREASE OF THE DE RITIS RATIO?

A

VIRAL AND TOXIC HEPATITIS

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87
Q

WHAT DISEASE IS ASSOCIATED WITH HIGHEST ELEVATION OF DERITIS RATIO

A

ACUTE HEPATITIS

88
Q

WHAT DISEASE IS ASSOCIATED WITH MODERATE ELEVATIONS OF DE RITIS RATIO

A

CHRONIC HEPATITIS, HEPATIC CARCINOMA, IM

89
Q

WHAT DISEASE IS ASSOCIATED WITH SLIGHT ELEVATIONS OF DE RITIS RATIO?

A

HEPATIC CIRRHOSIS, ALCOHOL HEPATITIS, AND OBSTRUCTIVE JAUNDICE

90
Q

> 2 DE RITIS RATIO

A

ALCOHOLIC HEPATITIS AND HEPATOCELLULAR DISORDER

91
Q

1-2 DERITIS RATIO

A

ACUTE HEPATITIS AND CIRRHOSIS

92
Q

<1 DERITIS RATIO

A

VIRAL HEPATITIS

93
Q

WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE

A

3.2.1.1

94
Q

MAJOR TISSUE SOURCES OF AMYLASE

A

PANCREAS AND SALIVARY CELLS OR GLANDS

95
Q

WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE

A

ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE

96
Q

WHAT ARE THE MINOR TISSUE SOURCES FOR AMYLASE

A

ADIPOCYTES, FALLOPIAN TUBE, SMALL INTESTINE, AND SKELETAL MUSCLE

97
Q

OTHER NAME FOR AMYLASE

A

4-1 GLUCAN-4-GLUCOHYDROLASE

98
Q

IN AMYLASE, PHYSIOLOGIC DIGESTION OF __ AND __ IS OBSERVED

A

STARCH AND GLYCOGEN

99
Q

THIS IS CONSIDERED AS THE SMALLEST ENZYME

A

AMYLASE

100
Q

TRUE OF FALSE: AMYLASE IS 15% HIGHER IN FEMALES

A

TRUE

101
Q

THIS IS THE MOST PREDOMINANT PANCREATIC AMYLASE ISOENZYME IN THE ONSET OF ACUTE PANCREATITIS

A

P3 ISOENZYME

102
Q

WHAT IS THE OTHER NAME FOR AMS SALIVARY TYPE

A

PTYALIN

103
Q

WHERE DOES PTYALIN MIGRATE IN ELECTRODE

A

ANODAL

104
Q

PATHOLOGIC INCREASE OF THE SALIVARY AMYLASE

A

PAROTITIS

105
Q

OTHER NAME FOR THE PANCREATI AMYLASE

A

AMYLOPSIN

106
Q

MIGRATION OF PANCREATIC AMYLASE IN THE ELECTRODE

A

CATHODAL

107
Q

PATHOLOGICAL INCREASE OF PANCRATIC AMYLASE

A

PANCREATITIS

108
Q

WHAT IS ADDED TO THE SPECIMEN TO AVOID THE INACTIVATION OF THE AMYLASE?

A

DILUTION WITH NACL

109
Q

WHAT ENDOGENOUS INHIBITORS ARE FOUND IN THE SERUM THAT INHIBIT AMYLASE

A

WHEAT GERM LECTIN AND TAG

110
Q

WHAT ANTICOAGULANTS INTERFERE WITH AMYLASE

A

CITRATE, EDTA, OXALATE

111
Q

WHAT SUBSTRATE IS USED IN AMYLASE DETERMINATION

A

STARCH

112
Q

this test for amylase measures the reducing sugar produced form hydrolyzed starch

A

SACCHAROGENIC

113
Q

THIS TEST FOR AMYLASE MEASURES THE DRECREASE IN SUBSTRATE CONNCENTRATION WHERE THE TIME OF DECOLORIZATION = THE AMOUNT OF AMYLASE ANCITVITY

A

AMYLOCLASTIC

114
Q

THIS TEST FOR AMYLASE MEASURES THE INCREASE IN COLOR INTENSITY WHERE THE AMOUNT OF STARCH DYE DESTROTYED = INTENSITY OF COLOR = AMOUNT OF AMYLASE ACTIVITY

A

CHROMOGENIC

115
Q

THIS METHOD OF AMYLASE DETERMINATION USES A-GLUCOSIDASE AND HEXOKINASE

A

COUPLED ENZYME

116
Q

THERE ARE 6 BANDS FORMED IN THE ELECTROPHORESIS OF AMYLASE: WHAT ARE THE FIRST 3 BANDS AND THE LAST 3 BANDS?

A

FIRST: PTYALIN
LAST: AMYLOPSIN

117
Q

THE INHIBITION TEST FOR AMYLASE USES TRITICUM VULGARIS AND IT INHIBITS WHAT TYPE OF AMYLASE

A

S TYPE OR SALIVARY TYPE OR PTYALIN

118
Q

WHAT IS THE FORMULA FOR AMYLASE CREATININE RATIO:

A

URINE AMYLASE/ SERUM AMYASE X SERUM CREATININE/ URINE CREATININE

119
Q

WHAT IS THE NORMAL REF RANGE FOR AMYLASE CREATININE RATIO?

A

1-5%

120
Q

INCREASED AMYLASE IN BLOOD AND URINARY SECRETIONS INDICATE WHAT DISEASE. AC RATIO IS 4-15%

A

ACUTE PANCREATITIS

121
Q

WHAT DISEASE CAUSES ELEVATED AMYLASE IN THE SERUM AND NORMAL AMYLASE URINARY SECRETION? THIS IS CHARACTERIZED BY AMYLASE BOUNDED TO AN ANTIBODY

A

MACROAMYLASEMIA

122
Q

WHAT IS THE ENZYMATIC NOMENCLATURE FOR LIPASE

A

3.1.1.3

123
Q

MAJOR TISSUE SOURCE OF LIPASE

A

PANCREAS

124
Q

ANOTHER NAME FOR LIPASE

A

TRIAGLYCEROL ACYLHYDROLASE

125
Q

IT INVOLVES THE HYDROLYSIS OF FATS TO PRODUCE ALCOHOL AND FATTY ACIDS

A

LIPASE

126
Q

THIS IS THE SUPERIOR DIAGNOSTIC TOOL FOR PANCREATITIS

A

LIPASE

127
Q

THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE

A

ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS

128
Q

THIS DISEASE CAUSES LOSS OF AMYLASE AND LIPASE

A

ACINAR CELL DEGRADATION FRO CHRONIC PANCREATITIS

129
Q

DECREASED LIPASE VALUES ARE OBSERVED IN

A

PANCREATIC INSUFFICIENCY

130
Q

WHAT SUBSTRATE IS USED FOR LIPASE MEASUREMENT

A

OLIVE OIL

131
Q

WHAT IS AN ALTERNATIVE FOR THE SUBSTRATE IN LIPASE MEASUREMENT

A

TIOLEIN OIL

132
Q

THIS INCREASES THE SENSITIVITY AND SPECIFICITY OF LIPASE MEASUREMENT

A

BILE SALTS AND COLIPASE

133
Q

THIS PREVENTS LIPASE INACTIVAITON

A
134
Q

THIS PREVENTS LIPASE INACTIVATION

A

COLIPASE

135
Q

INHIBITOR FOR LIPASE AND WHICH CAUSES FALSE DECREASE OF LIPASE

A

HEMGLOBIN (HEMOLYSIS)

136
Q

BACTERIAL CONTAMINATION CAUSES __ IN LIPASE VALUES

A

FALSE INCREASE

137
Q

WHAT IS THE REFERENCE METHOD FOR LIPASE DETERMINATION

A

CHERRY CRANDAL METHOD

138
Q

THIS USES OLIVE OIL IN THE DETERMINATION OF LIPASE. INCUBATIION IS DONE FOR 24 HOURS AT 37 C

A

CHERRY CRANDAL METHOD

139
Q

THIS IS COMMONLY USED METHOD FOR LIPASE MEASUREMENT; IT IS A COLORIMETRIC METHOD THAT USES MINIMAL SAMPLE

A

peroxidase coupling

140
Q

THIS LIPASE MEASUREMENT USES BOTH OLIVE AND TIROLEIN OIL

A

TURBIDIMETRY

141
Q

TRUE OR FALSE: DECREASE IN TURBIDIMETRY INDICATE DECREASE IN LIPASE?

A

FALSE. DECREASE IN TURBIDIMETRY = INCREASE LIPASE

142
Q

WHAT IS THE ENZYMATIC NOMENCLATURE FOR LACTATE DEHYDROGENASE?

A

1.1.1.27

143
Q

THIS ENZYME CAUSES THE INTERCONVERSION OF LACTIC AND PYRUVIC ACID

A

LACTATE DEHYDROGENASE

144
Q

WHAT COENZYME IS USED IN LACTATE DEHYDROGENASE?

A

NAD NICOTINAMIDE ADENINE DINUCLEOTIDE

145
Q

WHAT ARE THE 2 FORMS OF LACTATE DEHYDROGENASE

A

H AND M FORMS

146
Q

WHAT ARE THE MAJOR ISOENZYMES OF LACTATE DEHYDROGENASE

A

LD1, 2, 3, 4, 5

147
Q

WHAT IS THE MINOR ISOENZYME OF LACTATE DEHYDROGENASE

A

LD6

148
Q

WHAT LD ISOEZNYME HAS THE HIGHEST PERCENTAGE

A

LD2

149
Q

what are the sources of LD1 and LD2 clue: RK heart

A

RBC KIDNEY AND HEART

150
Q

WHAT ISOENZYME OF LD IS THE MOST ANODAL AND HEAT STABLE

A

LD 1 AND 2

151
Q

WHAT ARE THE SOURCES OF LD 2 (CLUE PLS)

A

PANCREAS LUNGS SPLEEN

152
Q

THE IS THE INTERMEDIARY ISOENZYME. MODERATE IN ABUNDANCE

A

LD3

153
Q

WHAT ARE THE SOURCES OF LD 4 AND 5 (CLUE SSmILI)

A

SKIN, SMALL, ILIUM, LIVER, INSTESTINE

154
Q

THIS IS THE LEAST ANODAL AND AND HEAT LABILE AND ARE SCARCE

A

LD 4 AND LD 5

155
Q

WHAT IS ANOTHER NAME FOR LD 6

A

ALCOHOL DEHYDROGENASE

156
Q

AT WHAT BAND DOES THE LD6 APPEAR IN ELECTROPHORESIS

A

6TH BAND

157
Q

THIS IS OBSERVED WHEN THERE IS HIGHEST ELEVATIONS OF LACTATE DEHYDROGENASE

A

PERNICIOUS ANEMIA

158
Q

NORMAL ELECTROPHORETIC PATTERN OF SERUM LD AS WELL AS WHEN THERE IS SEIZURE

A

2>1>3>4>5

159
Q

CSF AND ACUTE MYOCARDIAL INFARCTION LD ELECTROPHORETIC PATTERN

A

1>2>3>4>5

160
Q

LD ELETROPHORETIC PATTERN FOR BACTERIAL MENINGITIS

A

5>4>3>2>1

161
Q

WHAT LD ISOENZYMES ARE CONSIDERED AS CANCER MARKERS.

A

LD 2 3 4

162
Q

WHAT LD IS ASSOCIATED WTH HEPATIC CARCINOMA ANND TOXIC HEPATITIS

A

ld 5

163
Q

THIS ENZYME IS SEVERELY AFFECTED BY HEMOLYSIS

A

LD LACTATE DEHYDROGENASE

164
Q

LD LEVEL (>/

A

>

165
Q

TRUE OR FALSE: THERE IS DECREASE VALUE OF LD IF SAMPLE IS FROZEN

A

TRUE. THE STORAGE FOR LD IS AT ROOM TEMP 20 TO 26 C

166
Q

THIS METHOD FOR LD CONVERTS LACTATE TO PYRUVATE AT PH 8.8 AND THIS IDENTIFIES LD 1

A

WACKER METHOD

167
Q

THIS LD METHOD CONVERTS PYRUVATE TO LACTATE AT PH 7.2 AND IDENTIFIES LD 5

A

WROBLEWSKI LADUE (REVERSE METHOD

168
Q

IN HEAT DENATURATION FOR LD MEHTODS WHAT ISOENZYME IS STABLE

A

LD 1

169
Q

WHAT IS THE ISOENZYME NOMENCLATURE FOR CREATINE KINASE

A

2.7.3.2

170
Q

MAJOR TISSUE SOURCES FOR CREATINE KINASE

A

CARDIAC MUSCLE, SMOOTH MUSCLE, SKELETAL MUSCLE AND BRAIN

171
Q

TRUE OR FALSE: ATHLETES HAVE INCREASE BASELINE LEVEL OF CREATINE KINASE

A

TRUE

172
Q

THIS CAUSES INCREASE OF CK LEVELS FOR UPTO 5 TIMES

A

INTRAMUSCULAR INJECTION

173
Q

TRUE OR FALSE: BEDRIDDEN PATIENTS HAVE DECREASED CK VALUES

A

TRUE

174
Q

THIS IS A MINOR ISOENZYME OF CK SEEN IN ADVANCED MALIGNANCIES

A

MITOCHONDRIAL CK

175
Q

WHAT ARE THE DIFFERENT ISOZENZYMES OF CK

A

CK-BB CK-MB AND CK-MM

176
Q

THE MOST ANDODAL AND MOST LABILE CK ISOENZYME

A

CK BB

177
Q

HOW MANY PERCENT OF CK-MB IS PRESENT IN CARDIAC TISSUE?

WHAT PERCENT OF TOTAL CK INDICATES MYOCARDIAL DAMAGE

A

20% OF CK MBB IS PRESENT IN CARDIAC TISSUE

>6% OF TOTAL CK = MYOCARDIAL DAMAGAE

178
Q

THIS IS THE MAJOR ISOENZYME OF CK IN THE SERUM

A

CK-MM

179
Q

THIS IS THE LEAST ANODAL CK ISOENZYME

A

CK-MM

180
Q

HIGHEST ELEVATIONS OF CK IS SEEN IN THIS DISEASE

A

DUCHENE DISORDER

181
Q

PERCENTAGE OF TOTAL CK ATTRIBUTED TO CK-MB

POSSIBLE RELEASE OF CK-MB FROM NON-CARDIAC TISSUE

A

CK RELATIVE INDEX

182
Q

CK ACTIVATOR

A

N-ACETYL-CYSTEINE AND MG

183
Q

WHAT SUBSTANCE IS FOUND IN THE RBC CAUSING INCREASED CK

A

ADENYLATE KINASE

184
Q

INHIBITOR OF CK

A

URATE AND CYSTEINE

185
Q

TRUE OR FALSE: CK IS PHOTOSENSITIVE

A

TRUE

186
Q

CK WOULD BE DECREASED DURING STORAGE, WHAT REAGENTS ARE USED TO REMEDY THIS

A

CLELAND’S REAGENT AND GLUTATHIONE

187
Q

THIS IS THE FORWARD METHOD IN DETECTING CK. WHAT PH AND WAVELENGTH IS USED?

A

TANZER-GILOVARD METHOD | pH 9 | 340 nm

188
Q

THIS IS THE INDIRECT METHOD IN MEASURING CK. WHAT PH AND WAVELENTH IS USED?

A

OLIVER-ROSALSKI METHOD
PH6.8
340NM

189
Q

WHAT IS THE ENZYME NOMENCLATURE FOR GGT

A

2.3.2.1

190
Q

THIS ENZYME IS AN INDICATOR FOR ALCOHOLISM

A

GGT

191
Q

HIGHEST ELEVATIONS OF GGT

A

PRIMARY BILIARY CIRRHOSIS

192
Q

WHAT METHOD IS USED FOR GGT DETERMINATION AND WHAT WAVELENGTH IS USED?

A

SZASZ ASSAY

405-410 nm

193
Q

WHAT IS THE ENZYME NOMENCLATURE FOR TRUE AND PSEUDO CHOLINESTERASE

A
  1. 1.1.7 TRUE CHOLINESTERASE

3. 1.1.8 PSEUDOCHOLINESTERASE

194
Q

WHAT IS ANOTHER NAME FOR TRUE CHOLINESTERASE

A

ACYLCHOLINE ACYLHYDROLASE

195
Q

WHAT IS ANOTHER NAEM FOR PSEUDOCHOLINESTERASE

A

ACETYLCHOLINE ACETYLHYDROLASE

196
Q

THIS ENZYME IS USED IN THE ASSESSMENT OF PARENCHYMAL FUNCTION

A

CHOLINESTERASE

197
Q

INHIBITOR FOR CHOLINESTERASE

A

SUCCINYLCHOLINE

198
Q

WHAT IS THE MOST USED METHOD IN CHOLINESTERASE? WHAT WAVELENGTH IS USED?

A

ELLMAN

410 NM

199
Q

THIS METHOD IN CHOLINESTERASE DETERMINATION MAKE USE OF A SPECIALIZED EQUIPMENT

A

MICHEL

200
Q

WHAT IS THE ENZYME NOMENCLATURE FOR AMYLASE

A

4.1.2.13

201
Q

WHAT IS OTHER NAME FOR ALDOLASE

A

FRUCTOSE 1-6 DIPHOSPHATE ALDOLASE

202
Q

ISOENZYMES OF ALDOLASE AND WHERE ARE THEY FOUND?

A

ALDOLASE A: SKELETAL MUSCLE
ALDOLASE B : WBC, LIVER, KIDNEY
ALDOLASE C: BRAIN TISSUE

203
Q

WHAT IS THE MAIN TISSUE SOURCE OF OCT

A

LIVER

204
Q

WHAT METHOD IS USED FOR OCT

A

REICHARD AND REICHARD

205
Q

WHAT IS THE ENZYMATIC NOMENCLATURE FOR OCT

A

2.1.1.3

206
Q

WHAT ARE THE MAIN SOURCES OF LEUCINE AMINOPEPTIDASE

A

BUS

BILE URINE SERUM

207
Q

WHAT IS THE METHOD FOR LEUCINE AMINOPEPTIDASE

A

GOLDBERG AND RUTENBERG

208
Q

WHAT ARE THE LABORATORY METHODS FOR 5NT

A

DIXON AND PURDON
BELLFIELD AND GLODBERG
CAMPBELL

209
Q

ACE NOMENCLATURE

A

3.4.15.1

210
Q

MAIN SOURCE OF ACE

A

MACROPHATGES AND EPITHELOID CELLS

211
Q

THIS ENZYME IS INCREASED IN ALZHEIMERS DISEASE IN VIA CSF ANALYSIS

A

ACE

212
Q

CERULOPLASMIN NOMENCLATURE

A

1.16.3.1

213
Q

ENZYME THAT IS A KNOWN MARKER FOR WILSONS DISEASE

A

CERULOPLASMIN

214
Q

THIS ENZYME MAINTAINS THE NADPH IN A REDUCED FROM INSIDE THE RBC

A

G6PD

215
Q

G6PD NOMENCLATURE

A

1.1.1.49

CLINICAL CHEMISTRY (2 decks)

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