Enzymes Kinetics Flashcards

1
Q

what is the stoichiometirc rate euqation

A
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2
Q

what are the elemetary steps

A
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3
Q

what is the order of a reaction

A
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4
Q

what is the molecularity of the elementary steps

A
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5
Q

what is velocity

A

it is the reaction rate which is equal to the change in concentration products as a function of time

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6
Q

what is Zero-order kinetics

A
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7
Q

why is Zero-order kinetics associated with enzyme kinetics

A
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8
Q

what is 1st order

A
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9
Q

what is 2nd order

A
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10
Q

what is 3rd order

A
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11
Q

what are the initial velocity conditions used to measure steady-state enzyme kinetics

A
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12
Q

what is the rapid equilibrium assumptions

A
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13
Q

how is it used to derive the Michaelis Menten equation

A
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14
Q

what is the steady state assumptions

A

h

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15
Q

How is it used to derive the Michaelis Menten equation

A
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16
Q

what is the steady state assumption

A
17
Q

how is it used to derive the Michaelis Menten equation

A
18
Q

What is the Michealis Menten Equation

A
19
Q

What is Km

A

it is the kinetic activator constant`

20
Q

what is Vmax

A

the theoretical maximal velocity

21
Q

what is the turnover number

A

A measure of catalytic activity

22
Q

what are the 4 types of reversible enzyme inhibition

A
  1. competitive
  2. noncompetitive
  3. mixed noncompetitive
  4. uncompetitive
23
Q

what type of inhibition pattern is observed when the Lineweaver Burke Plots are constructed for an irreversible inhibitor

A
24
Q

what is specific acid-base catalysis

A
25
Q

what is general acid-base catalysis

A
26
Q

what is the mechanism for RNAase

A
27
Q

What is the role of the two histidine in Catalytic mechanisms

A
28
Q

why doesn’t RNAas hydrolyze with DNA

A
29
Q
A
30
Q
A