Enzymes Kinetics Flashcards
what is the stoichiometirc rate euqation
what are the elemetary steps
what is the order of a reaction
what is the molecularity of the elementary steps
what is velocity
it is the reaction rate which is equal to the change in concentration products as a function of time
what is Zero-order kinetics
why is Zero-order kinetics associated with enzyme kinetics
what is 1st order
what is 2nd order
what is 3rd order
what are the initial velocity conditions used to measure steady-state enzyme kinetics
what is the rapid equilibrium assumptions
how is it used to derive the Michaelis Menten equation
what is the steady state assumptions
h
How is it used to derive the Michaelis Menten equation
what is the steady state assumption
how is it used to derive the Michaelis Menten equation
What is the Michealis Menten Equation
What is Km
it is the kinetic activator constant`
what is Vmax
the theoretical maximal velocity
what is the turnover number
A measure of catalytic activity
what are the 4 types of reversible enzyme inhibition
- competitive
- noncompetitive
- mixed noncompetitive
- uncompetitive
what type of inhibition pattern is observed when the Lineweaver Burke Plots are constructed for an irreversible inhibitor
what is specific acid-base catalysis
what is general acid-base catalysis
what is the mechanism for RNAase
What is the role of the two histidine in Catalytic mechanisms
why doesn’t RNAas hydrolyze with DNA
