Enzymes I

Question 1

Define the terms: holoenzyme, apoenzyme, coenzyme, cofactor, activation energy.

Answer 1

.

Question 2

Describe the mechanism of enzyme activity.

Answer 2

.

Question 3

Classify enzyme (6 classes) nomenclature based on activity.

Answer 3

.

Question 4

Differentiate between the lock and key and the induced fit model of enzyme-substrate binding.

Answer 4

.

Question 5

Differentiate between the lock and key and the induced fit model of enzyme-substrate binding.

Answer 5

.

Question 6

Differentiate between the binding site and the catalytic site.

Answer 6

.

Question 7

Explain the effect of the following factors on enzyme activity: substrate concentration, enzyme concentration, pH, temperature, and inhibitors/activators

Answer 7

.

Question 8

Identify the various regulatory mechanisms of enzyme activity: proteolytic cleavage, allosteric , covalent modification upregulatio/induction, repression/ down regulation

Answer 8

.