enzymes/digestion/plant organisation Flashcards
revision
- enzymes are biological………. that speed up ………… with out being ……….. themselves.
- enzymes work using the lock and key theory
catalysts, chemical reactions, used up
- the active site of the enzyme has a ………… shape to the
substrate - each enzyme is specific to only one ……….
complimentary
substrate
enzymes in digestion
the enzyme amylase substrate is …… the products are….. they are produced in the ……. the product works in the ……..
starch, simple sugars, pancrease and small intestine, mouth
the enzyyme amylase substrate is starch the products of this are simple sugars. they are produced in the small intestine and pancrease the product works in the mouth.
enzymes in digestion
the enzyme protease substrate is ……. the products are ……. these are produced in the ……… and ……… they work in the ………
proteins, amino acids, pancrease and small intestine, stomach
the enzyme protease substrate is proteins the products are amino acids these are produced in the pancrease and small intestine they work in the stomach.
enzymes in digestion
the enzyme lipase substrate is ……. the products are …….. and …….. they are produced in the ……. and ……… they work in the ………..
lipids, glycerol fatty acids, pancrease and small intestine, small intestine
the enzyme lipases substarte is lipids the products are glycerol and fatty acids they are produced in the pancrease and small intestines they are used in the small intestine.
required practical- effect of ph on amylase activity
- add a drop of ……. to each well of a spotting tile
- place 3 test tubes containing solutions of …… and ……… and ph buffer into a water bath at ….. degrease celcius leave for 5mins to ………..
- mix amylase and ph …… solutions then add the …. solution and start a stop watch.
- every 30 seconds use a …… to remove a drop of the mixture and add to the iodine in the spotting tile
- repeat until the iodine remains …….. and does not change colour
- repeat step 1-5 using a different ph…….. and the same temp water and …….. of the solutions.
iodine
amylase and starch, 35, reach the right temp
buffer, starch
pippet
orange
buffer
volume
to more accuratley find the optimum ph you should test smaller intervals between ph 6 and 7