Enzymes and Restriction Mapping Flashcards
What are 3 recombinant proteins that are made using genetic engineering?
→ Insulin
→ Interferon
→ G-CSF
What is G-CSF?
→ produced by infected cells and induces antiviral defense
What do nucleases do?
→ Degrade nucleic acids by hydrolysing phosphodiester bonds
What does ribonuclease degrade?
→ RNA
What does deoxyribonuclease degrade?
→ degrades DNA
What do exonucleases degrade?
→ degrades from the end of the molecule
What do endonucleases degrade?
→ degrades from the middle of the molecule
What is the purpose of restriction?
→ to limit the transfer of nucleic acids from infecting phages into bacteria
What 2 things do restriction endonucleases do?
→Recognize a specific sequence
→ cut a specific sequence
What sequence does ECOR1 recognise?
→ GAATTC
What are the characteristics of recognition sites?
→ 4-8 base pairs in length
→ they are palindromic
How often does a 4 base sequence occur?
→ every 265 bases
When does a 6 base recognition sequence occur?
→ every 4096 base pairs
What 2 nucleases produce an overhang?
→ EcoR1
→ Kpn1
What nuclease does not produce an overhang?
→Alu 1