Enzymes Flashcards
Theories of mechanism of activation?
1) Lowering of activation energy
2) Fischer’s template theory
3) Michaelis menten theory
4) koshland’s induced fit theory
Class 1 of enzymes?
Oxidoreductase
Class 2 of enzymes
Transferases
Class 3 of enzymes
Hydrolase
Class 4 of enzymes
Lyase
Class 5 of enzymes
Isomerases
Class 6 of enzymes
Ligases
Factors influencing enzyme activity?
1)Velocity proportional to enzyme Concentration
2) temperature:bell shaped curve
Increasing :with increase rate of reaction
Decreasing :dt denaturation of protiens
3) substrate concentration/saturation concentration.
Hyperbolic curve
4) Michaelis-menten equation
Velocity of reaction varies with substrate concentration
Vi=(vmax * s) / km + s
5)lineweaver-burk plot
Double reciprocal plot
Y= a x + b
Stating (CI)inhibits? And clinical use
- Hmg coA
- lowering cholesterol
Dicumora(CI)l inhibits and use?
Vit k epoxide reductase
Anti coagulant
Allopurinol (CI)inhibits and use
Xanthine oxidase
Gout
Penicillin (CI)inhibits and use
Transpeptidase
Antibiotic
Sulfonamide (CI) inhibits and use?
Pteroid synthetase
Antibiotic
Trimethoprim(CI) inhibits and use?
Dihydrofolate
Antibiotic
Primethamine (CI)inhibits and use
FH2 reductase
Malaria