Enzymes

Question 1

Describe the induced fit model, why is this better than the lock and key model?

Answer 1

Active site forms because of interactions between the enzymes and the substrate, substrate triggers a change in the protein structure of the enzyme
Better than lock and key because enzymes aren’t rigid structures

Question 2

Describe the effect of pH on an enzyme

Answer 2

Enzymes have an optimum pH
Change from optimum leads to charges being changed on amino acids which disrupts ES-Complex formation
May also cause hydrogen bonds in tertiary structure to break

Question 3

Describe the relation between enzyme and substrate concentrations on rate of enzyme catalysed reactions

Answer 3

An Enzyme concentration increase will lead to proportional rate increase until the substrate becomes a limiting factor, the opposite is true for substrate concentration

Question 4

Differenatiate between competitive and non competitive inhibition

Answer 4

Competitive is where the inhibitor works on the active site
Non competitive is where the inhibitor binds elsewhere
Non competitive inhibitors can not be tackled with increasing substrate concentration