enzymes

Question 1

aim

Answer 1

how temp affects enzyme activity

Question 2

method for amylase

Answer 2

• add iodine to spotting tile
• mix starch solution and amylase in test tube
• place in water bath at specific temp
• every 30s take a drop and add to iodine
• if iodine stays brown starch is gone so record the time taken
• repeat for other temp

Question 3

IV and DV

Answer 3

IV: temp
DV: time taken for starch to be broken down

Question 4

control variables

Answer 4

• vol. and conc. of starch and amylase (substrate and enzyme vol. and conc.
• same buffer solution
• time intervals

Question 5

improvements

Answer 5

• water bath
• colorimeter
• buffer - maintain constant pH
• repeat and calc. mean

Question 6

results

Answer 6

• low temp = less KE - few collisions
• opt temp = max rate
• high temp = denatures - AS changes - ROR decreases