Enzymes Flashcards

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1
Q

Define enzyme

A

Enzymes are protein catalysts that speed up a reaction without being used up in the reaction

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2
Q

What organelle produces enzymes

A

Ribosome

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3
Q

What are proteins formed from

A

Proteins are formed by a sequence of amino acids forming a long chain

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4
Q

What type of protein are enzymes

A

Globular proteins

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5
Q

Define the enzymes shape

A

enzymes have a folded 3-dimensional shape

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6
Q

What is the active site

A

The part of the enzyme that joins with the substrate

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7
Q

What is a substrate

A

The substance with which an enzyme reacts

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8
Q

What is a product

A

The substance which the enzyme forms

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9
Q

Define metabolism

A

The sum of all chemical reactions within the cells of living things

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10
Q

What are the two metabolic reactions

A

Anabolic & Catabolic

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11
Q

What is a catabolic reaction

A

The breakdown of larger molecules into smaller molecules releasing energy

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12
Q

What is an anabolic reaction

A

The build up of larger molecules from smaller molecules using energy

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13
Q

Catabolic reaction from animals

A

Digestion

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14
Q

Catabolic reaction from plants

A

Respiration

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15
Q

Anabolic reaction from animals

A

Protein synthesis

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16
Q

Anabolic reaction from plants

A

Photosynthesis

17
Q

Lock and key theory

A

Enzymes will fit neatly and react only with a substance that matches the enzymes shape (the 3 diagrams)

18
Q

Chemical nature/ Biomolecule in enzymes

A

The chemical nature/Biomolecule is protein

19
Q

Examples of Catabolic enzymes

A

Lipase acts on lipids (Ph 7/8)
Amylase acts on starch (Ph 7/8)
Pepsin acts on protein (Ph 2)

20
Q

Examples of anabolic enzymes

A

Dna ligase : joins two pieces of dna together
Dna polymerase : forms & repairs dna

21
Q

Factors affecting enzyme activity

A

Temparture & Ph

22
Q

Define optimum activity

A

The rate at which an enzyme works best at

23
Q

What temperature do human enzymes work best at

A

37 degrees

24
Q

What temperature do plant enzymes work best at

A

20 - 30 degrees

25
Q

What happens to enzymes if outside of their optimum temperature

A

The enzymes rate of activity reduces and begins to denature. Above a certain temperature enzymes lose their 3 dimensional shape

26
Q

Define Ph as a factor

A

Enzymes work over a very narrow ph range. Most enzymes work best between (Ph 6-8)

27
Q

Define a denatured enzyme

A

A denatured enzyme has lost its shape and can no longer function

27
Q

What is denutration caused by

A

Denutration is caused by unsuitable temperature and Ph

28
Q

Define immobilised enzymes

A

Immobilised enzymes are attached or fixed to each other or in an insert material

29
Q

Define bio processing

A

Bio processing is the use of enzyme controlled reactions to produce a product

30
Q

Define bioreactor

A

A bioreactor is a vessel or container in which living things or their products are used to make a product

31
Q

Advantages of immobilised enzymes

A

Cheaper
Can be reused
Increased stability - less enzymes needed

32
Q

Define heat denutration

A

The shape of the enzyme is permanently changed and loses its function

33
Q

Uses/Application of immobilised enzymes

A

Glucose isomerise is used to convert glucose (substrate) into fructose (product)

Glucose - Glucose isomerise - Fructose