Enzymes Flashcards

1
Q

the nucleic acids which behave like enzymes are called

A

ribozymes

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2
Q

an _______ ______ of an enzyme is the crevice or pocket into which the substrate fits

A

active site

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3
Q

difference between inorganic catalysts and enzymes

A

inorganic catalysts work efficiently at high temperatures and high pressures but enzymes get damaged above 40 degree C

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4
Q

enzymes isolated from organisms who normally live under extremely high temperatures (example; ______ and ______) are stable and retain their catalytic power even at high temperatures (example; ____ , _____)

A

hot vents and sulphur springs
80 to 90 degree C

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5
Q

_____ _____ is an important quality of such enzymes isolated from thermophilic organisms

A

thermal stability

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6
Q

hydrolysis of starch into ____ is an organic chemical reaction

A

glucose

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7
Q

rates of physical and chemical processes are influenced by ______ among other factors

A

temperature

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8
Q

a general rule of thumb is that rate doubles or decreases by half for every _____ change in either direction

A

10 degree C

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9
Q

in the reaction of carbion dioxide and water forming carbonic acid, the enzyme used is

A

carbonic anhydrase

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10
Q

reaction of formation of carbonic acid without the enzyme forms _____ molecules in ______ minutes

A

200
60

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11
Q

reaction of formation of carbonic acid with the enzyme forms _____ molecules in _____ seconds

A

6,00,000
1

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12
Q

with carbonic anhydrase, the rate of reaction increases by _____ times

A

10 million

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13
Q

example of metabolic pathway

A

glucose to pyruvic acid

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14
Q

in our skeletal muscle under anaerobic conditions ___ is formed

A

lactic acid

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15
Q

in our skeletal muscle under normal aerobic conditions, ___ is formed

A

pyruvic acid

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16
Q

in yeast, during fermentation, ____ is formed

A

ethanol

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17
Q

during the state where substrate is bound to the enzyme’s active site, a new structure of the substrate is formed called

A

transition state structure

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18
Q

if potential energy of product is lesser than potential energy of its substrate, then the type of reaction is

A

exothermic

19
Q

do exothermic reactions need energy to form the product

20
Q

factors affecting enzyme activity are

A

temperature
ph
change in substrate concentration
binding of specific chemicals that regulate its activity

21
Q

low temperature preserves the enzymes in a _____ ______ state

A

temporarily inactive

22
Q

high temperature destroys the enzymatic activity because

A

proteins are denatured by heat

23
Q

when the binding of the chemical shuts off enzyme activity, the process is called _____ and the chemical is called an ______

A

inhibition
inhibitor

24
Q

when the inhibitor closely resembles the substrate in its molecular structure and inhibits the activity of the enzyme, it is known as

A

competitive inhibitor

25
example of competitive inhibition
enzyme : succinic dehydrogenase inhibitor : malonate substrate: succinate
26
competitive inhibitors are often used in the control of
bacterial pathogens
27
how are enzymes named
by a four-digit number
28
6 classes of enzymes
oxidoreductases transferases hydrolases lyases isomerases ligases
29
oxidoreductases are also called
dehydrogenases
30
role of oxidoreductases
catalyse oxidoreduction between two substrates
31
role of transferases
catalysing a transfer of a group other than hydrogen between a pair of substrates
32
role of hydrolases
catalysing hydrolysis between ester, ether, peptide, glycosidic, carbon-carbon, carbon-halide, phorphorus-nitrogen bonds
33
role of lyases
catalysing removal of groups from substrates in mechanisms other than hydrolysis and resulting in double bonds
34
role of isomerases
catalysing inter-conversion of optical, geometric and positional isomers
35
role of ligases
catalysing linking of two compounds eg. carbon-oxygen, carbon-sulphur, carbon-nitrogen, phosphorus-oxygen, etc bonds
36
when a cofactor is bound to an enzyme to make it catalytically active, the protein portion of the enzyme is called
apoenzyme
37
three kinds of cofactors for enzymes
prosthetic groups coenzymes metal ions
38
prosthetic groups are ______ compounds and distinguished from other cofactors because
organic they are tightly bound to the apoenzyme
39
role of peroxidase and catalase
catalyse the breakdown of hydrogen peroxide to water and oxygen
40
example of a prosthetic group
enzyme: peroxidase, catalase prosthetic group- cofactor: haem (part of active site)
41
coenzymes are _____ compounds but their association with the apoenzyme is only ____
organic transient
42
the essential chemical components of many coenzymes are ______ for eg; NAD and NADP contain _____
vitamins niacin
43
full form of NAD
nicotinamide adenine dinucleotide
44
example for metal ions as cofactor for enzymes
cofactor: zinc proteolytic enzyme: carboxypeptidase