enzymes Flashcards
define metabolism
the sum of all the enzyme controlled reactions taking place in a cell
what are the two main types of reactions that make up metabolism?
anabolic and catabolic reactions
what is an enzyme?
an enzyme is a biological catalyst used to speed up the rate of intercellular and extra cellular biochemical reactions. an enzyme cannot be used up or permanently altered
what is an intracellular enzyme?
an enzyme that acts within cells, for example catalase
what is the active site of an enzyme?
a region on the enzyme that is complementary to the shape of a specific substrate. the substrate binds and the reaction takes place
why is the active site described as ‘specific’?
only specific substrates complementary to the active site can bind.
the structure of each enzyme (including the active site) is unique due to the presence of different side chains and branches
define activation energy
the minimum amount of energy required for a reaction to take place
what is catalysis?
an increase in the rate of a chemical reaction using a catalyst such as an enzyme. the catalyst lowers the activation energy of the reaction
what factors affect the rate of an enzyme controlled reaction?
temperature, pH, substrate concentration, and enzyme concentration
what is a buffer?
a molecule that maintains a constant pH in a solution when small amounts of acid (H+) or base (OH-) are added