Enzymes Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Aim

A

Determine the time taken for starch to be digested and to calculate the rate of enzyme activity at different pH levels

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Step one

A

Add 2 cm³ of each solution ranging from pH 3 to 7 into each labelled test tube using a syringe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Step two

A

Immerse test tube, starch and Amylase solution in water bath at 25°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Step three

A

Place 2 cm³ of Aminé solution into test tube of buffer solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Step four

A

Place 2 cm³ of starch into test tube and start time

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Step five

A

Repeat step for every 30 seconds until the iodine solution remains brown and does not turn blue black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Step six

A

Calculate the rate of enzyme action using:
1 / Time taken for iodine solution to remain brown

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Step seven

A

Repeat steps 1–6 for different Ph values

How well did you know this?
1
Not at all
2
3
4
5
Perfectly