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biochem sem 1 > enzyme table > Flashcards

enzyme table Flashcards

1
Q

what is the enzyme group of this enzyme: Salivary α-amylase

A

Glycosidase
Endoglycosidase

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2
Q

what is the specific activator of this enzyme: Salivary α-amylase

A

Cl-

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3
Q

what is the substrate of this enzyme: Salivary α-amylase

A

Starch, glycogen

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4
Q

what is the action mechanism of this enzyme: Salivary α-amylase

A

Hydrolysis of internal
α 1,4 glycosidic bonds

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5
Q

potential products of this enzyme: Salivary α-amylase

A

Dextrins, maltotriose, maltose,
isomaltose

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6
Q

what is the pH of saliva?

A

6.8 – 7.2

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7
Q

where do we find this enzyme? Salivary α-amylase

A

Salivary/lingual
glands

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8
Q

what is the enzyme group of this enzyme: Lingual lipase (characteristic for newborns,
infants)

A

Esterase

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9
Q

what is the specific activator of this enzyme: Lingual lipase

A

none

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10
Q

what is the substrate of this enzyme: Lingual lipase

A

Triacylglycerides

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11
Q

what is the action mechanism of this enzyme: Lingual lipase

A

Hydrolysis of ester bonds

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12
Q

potential products of this enzyme: Lingual lipase

A

Fatty acids, 1,2-diacylglycerols,
rarely monoacylglycerols

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13
Q

where do we find this enzyme? lingual lipase

A

in Salivary/lingual glands

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14
Q

what is the pH of gastric juice?

A

1-2 (acidic)

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15
Q

what is the enzyme group for pepsin?

A

peptidase, endopeptidase

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16
Q

what is the specific activator for pepsin?

A

HCl, autocatalysis

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17
Q

what is the substrate for pepsin?

A

proteins

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18
Q

what is the action mechanism for pepsin?

A

Hydrolysis of peptide
bonds after aromatic AA
(Phe, Tyr, Trp)

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19
Q

potential products of pepsin?

A

Polypeptides, oligopeptides,
tripeptides, dipeptides

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20
Q

what is the optimum pH for Gastric lipase?

A

4-6

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21
Q

what is the enzyme group for Gastric lipase?

A

esterase

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22
Q

specific activator for Gastric lipase?

A

none

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23
Q

substrate for Gastric lipase?

A

Triacylglycerides

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24
Q

action mechanism for gastric lipase

A

Hydrolysis of ester bonds

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25
Q

potential products for gastric lipase:

A

Fatty acids, 1,2-diacylglycerols,
rarely monoacylglycerols

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26
Q

which kind of cells do we have in the stomach?

A

chief cells

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27
Q

which kind of cells do we have pancreas?

A

acinar cells

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28
Q

where do we find the enzyme pepsin?

A

in stomach

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29
Q

where do we find the enzyme gastric lipase?

A

in stomach

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30
Q

what is the enzyme group for Pancreatic α-amylase?

A

Glycosidase
Endoglycosidase

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31
Q

what is the specific activator for Pancreatic α-amylase?

A

Cl-, Ca2+ (for stabilization of active site)

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32
Q

what is the substrate for Pancreatic α-amylase?

A

Starch, glycogen

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33
Q

action mechanism for Pancreatic α-amylase?

A

Hydrolysis of internal
α 1,4 glycosidic bonds

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34
Q

potential products for Pancreatic α-amylase?

A

Dextrins, maltotriose, maltose,
isomaltose

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35
Q

what is the specific activator for Trypsin?

A

Enterokinase
(enteropeptidase)
and trypsin

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36
Q

what is the enzyme group for trypsin?

A

peptidase, endopeptidase

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37
Q

substrate for trypsin?

A

Proteins,
peptides

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38
Q

action mechanism for trypsin?

A

Hydrolysis of internal
peptide bonds after basic AA
(Arg, Lys, but NOT His)

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39
Q

potential products for trypsin?

A

Shorter polypeptides, peptides

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40
Q

where do we find trypsin?

A

in the pancreas

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41
Q

where do we find Pancreatic α-amylase?

A

in pancreas

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42
Q

where do we find chymotrypsin?

A

in pancreas

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43
Q

what is the pH of pancreatic juice?

A

7.6-8.3

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44
Q

what is the enzyme group of chymotrypsin?

A

peptidase, endopeptidase

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45
Q

specific activator for chymotrypsin?

A

Trypsin –
proteolytical
cleavage

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46
Q

substrate for chymotrypsin

A

Proteins,
peptides

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47
Q

action mechanism for chymotrypsin

A

Hydrolysis of internal
peptide bonds after aromatic
AA (Phe, Tyr, Trp)

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48
Q

potential products for chymotrypsin

A

Shorter polypeptides, peptides

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49
Q

where do we find elastase?

A

in pancreas

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50
Q

what is the enzyme group for elastase?

A

peptidase, endopeptidase

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51
Q

specific activator of elastase

A

Trypsin –
proteolytical
cleavage

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51
Q

substrate of elastase

A

Proteins
(polypeptides),
peptides

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52
Q

action mechanism of elastase

A

Hydrolysis of internal
peptide bonds after short AA
(Ser, Thr, Ala, Gly, Cys)

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53
Q

potential products of elastase

A

Shorter polypeptides, peptides

54
Q

enzyme group of Carboxypeptidase A

A

Peptidase,
Exopeptidase

55
Q

specific activator of Carboxypeptidase A

A

Trypsin –
proteolytical
cleavage

56
Q

substrate of Carboxypeptidase A

A

Proteins
(polypeptides),
peptides (smallest
tripeptide)

57
Q

where do we find Pancreatic lipase?

A

in pancreas

58
Q

what is the enzyme group of Pancreatic lipase?

A

esterase

59
Q

what is the substrate of pancreatic lipase?

A

Triacylglycerides

60
Q

what is the action mechanism of pancreatic lipase?

A

Hydrolysis of ester bonds

61
Q

what is the potential products of pancreatic lipase?

A

Fatty acids, monoglycerols
glycerol

62
Q

what is the enzyme group for Pancreatic lipase related
protein-2?

A

esterase

63
Q

what is the substrate for pancreatic lipase?

A

Triacylglycerides,
galactolipids, phospholipids

64
Q

what is the action mechanism for pancreatic lipase?

A

Hydrolysis of ester bonds

65
Q

what is the potential products of pancreatic lipase?

A

Fatty acids, monoacylglycerols,
monoacylgalactosylglycerol,
monoacylphosphoglycerol

66
Q

what is the enzyme group for Carboxyl ester lipase?

A

esterase

67
Q

what is the substrate for carboxyl ester lipase?

A

Triglycerides, galactolipids,
phospholipids, cholesterol
esters

68
Q

what is the action mechanism for carboxyl ester lipase?

A

Hydrolysis of ester bonds

69
Q

what is the potential products of carboxyl ester lipase?

A

Fatty acids, monoacylglycerols,
monoacylgalactosylglycerol,
monoacylphosphoglycerol cholesterol

70
Q

what is the enzyme group for Phospholipase A2?

A

Esterase

71
Q

what is the substrate for phospholipase A2?

A

Phospholipids

72
Q

what is the action mechanism for phospholipase A2?

A

Hydrolysis of ester bonds

73
Q

potential products for phospholipase A2?

A

Fatty acids, monophosphoglycerol

74
Q

the enzyme group for Polynucleotidase:
Ribonuclease
Deoxyribonuclease?

A

Esterase

75
Q

substrate for Polynucleotidase:
Ribonuclease
Deoxyribonuclease

A

RNA - polynucleotides
DNA - polynucleotides

76
Q

action mechanism for Polynucleotidase:
Ribonuclease
Deoxyribonuclease?

A

Hydrolysis of phosphodiester
bonds between nucleotides

77
Q

potential products for Polynucleotidase:
Ribonuclease
Deoxyribonuclease?

A

Oligonucleotides,
mononucleotides

78
Q

which kind of cells do we find in the intestinal mucose?

A

absorptive cells

79
Q

enzyme group for Glucoamylase?

A

Glycosidase,
Exoglycosidase

80
Q

substrate for glucoamylase

A

Oligosaccharides, dextrins,
maltotriose, maltose

81
Q

action mechanism for glucoamylase

A

Hydrolysis of terminal α-1,4-
glycosidic bonds at
nonreducing end

82
Q

potential products for glucoamylase

A

Shorter oligosaccharide, Glucose

83
Q

enzyme group for β – glycosidase complex?

A

Glycosidase,
Exoglycosidase

84
Q

substrate for β – glycosidase complex

A

Lactose, cellobiose, glycosyl-β-ceramides

85
Q

action mechanism for β – glycosidase complex

A

Hydrolysis of β 1,4 glycosidic
bond

86
Q

potential products for β – glycosidase complex

A

Glucose and galactose

87
Q

enzyme group for Sucrase-isomaltase complex

A

Glycosidase,
Exoglycosidase

88
Q

substrate for Sucrase-isomaltase complex

A

Dextrins, maltose, maltotriose,
isomaltose, sucrose

89
Q

action mechanism for Sucrase-isomaltase complex

A

Hydrolysis of α 1,2; both α-1,4-
and α-1,6-glycosidic bonds

90
Q

potential products for Sucrase-isomaltase complex

A

Glucose; fructose, shorter
oligoscahharides

91
Q

enzyme group for Trehalase

A

glycosidase, exoglycosidase

92
Q

substrate for trehalase

A

trehalose

93
Q

action mechanism for trehalase

A

Hydrolysis of α 1,1-glycosidic
bonds

94
Q

potential products for trehalase

A

Glucose

95
Q

enzyme group for nucleosidase

A

glycosidase

96
Q

substrate for nucleosidase

A

nucleoside

97
Q

action mechanism for nucleosidase

A

hydrolysis of N-glycosidic bond

98
Q

potential products for nucleosidase

A

Pentose (ribose or deoxyribose); purine or pyrimidine base

99
Q

enzyme group for aminopeptidase

A

peptidase, exopeptidase

100
Q

substrate for aminopeptidase

A

peptide (smallest tripeptide)

101
Q

action mechanism for aminopeptidase

A

hydrolysis of any AA from N terminal

102
Q

potential products of aminopeptidase

A

shorter peptide and amino acid

103
Q

enzyme group of dipeptidase

A

peptidase, exopeptidase

104
Q

substrate for dipeptidase

A

dipeptides

105
Q

action mechanism for dipeptidase

A

Hydrolysis of any dipeptide

106
Q

potential products of dipeptidase

A

amino acids

107
Q

enzyme group of nucleotidase

A

esterase

108
Q

substrate of nucleotidase

A

nucleotide

109
Q

action mechanism for nucleotidase

A

hydrolysis of ester bonds in nucleotide

110
Q

potential products of nucleotidase

A

nucleoside and phosphate

111
Q

enzyme group of Carboxypeptidase B

A

Peptidase,
Exopeptidase

112
Q

specific activator of Carboxypeptidase B

A

Trypsin –
proteolytical
cleavage

113
Q

substrate of Carboxypeptidase B

A

Proteins
(polypeptides),
peptides (smallest
tripeptide)

114
Q

action mechanism of Carboxypeptidase B

A

Hydrolysis of basic (Arg,
Lys, but NOT His) from
carboxy-terminal end

115
Q

potential products of Carboxypeptidase B

A

Shorter peptide (smallest
dipeptide) AND amino acid

116
Q

where do we find nucleotidase?

A

intestinal mucosa

117
Q

where do we find dipeptidase

A

intestinal mucose

118
Q

where do we find aminopeptidase

A

intestinal mucosa

119
Q

where do we find nucleosidase

A

intestinal mucosa

120
Q

where do we find trehalase

A

intestinal mucosa

121
Q

where do we find ssucrase-isomaltase complex

A

intestinal mucosa

122
Q

where do we find β – glycosidase complex?

A

intestinal mucosa

123
Q

where do we find glucoamylase?

A

intestinal mucosa

124
Q

where do we find Polynucleotidase:
Ribonuclease
Deoxyribonuclease?

A

in pancreas

125
Q

where do we find Phospholipase A2

A

in pancreas

126
Q

where do we find Carboxyl ester lipase?

A

in pancreas

127
Q

where do we find Pancreatic lipase related
protein-2?

A

in pancreas

128
Q

where do we find Pancreatic lipase?

A

in pancreas

129
Q

where do we find Carboxypeptidase B?

A

in pnacreas

130
Q

where do we find Carboxypeptidase A?

A

in pancreas

131
Q

where do we find Elastase?

A

in pancreas

132
Q

action mechanism of Carboxypeptidase A

A

Hydrolysis of aromatic AA
(Phe, Tyr, Trp) from carboxy-terminal end

133
Q

potential products of Carboxypeptidase A

A

Shorter peptide (smallest
product dipeptide) AND amino
acid

biochem sem 1 (8 decks)

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