Enzyme Kinetics Flashcards
What is V max?
The maximum velocity of a reaction (when all the enzyme has been used)
What is Km?
The concentration of substrate which is where Vmax is at 50%
In the reaction E + S = ES what are the two rate constants?
Forwards is K1 (enzyme association) and backwards is K-1 (enzyme dissociation)
In the reaction ES = E + P what represents the forward equation?
K2 - rate constant of enzyme conversion of substrate to product
What is the Michaelis Constant (Km)?
Km = (K-1 + K2)/K1
What is the Michaelis-Menten model?
[ES] = K1[E][S]/(K-1+K2)
How are Vmax and Km measured?
Measure initial reaction velocity (V0) at a known substrate concentration and repeat at increasing substrate concentration
How do you plot Vmax and Km on a graph?
Initial reaction rates (V0) are plotted as a function of substrate concentration. At infinite substrate concentration the initial reaction rate approaches a maximal rate Vmax. Take half of Vmax = Km
What are Vmax and Km as straight line components?
Vmax is the Y axis interception and Km is the x axis interception
How does level of Km affect substrate concentration?
Low Km means only a little substrate required and a high Km means a lot of substrate for half-maximal velocity
How do red blood cells and liver&pancreas both catalyse glucose conversion with different Km?
RBC: Hexokinase Km is 0.05mM glucose which maintains energy production by glycolysis even if glucose levels drastically fall
L&P: Glucokinase Km is 5mM glucose which enables glucose sensing and homeostasis. It’s abundance in the liver is regulated by insulin and excess blood glucose is metabolised
What is MODY?
Maturity Onset Diabetes of the Young - loss of glucokinase activity = loss of insulin-mediated glucose homeostasis
What is the transcription factor in oxygen regulation pf gene expression?
Hypoxia inducible factor
What is the mechanism in oxygen regulation of gene expression?
Oxygen sensors (prolyl hydroxylases) –> transcription factor (hypoxia inducible factor) –> genes for surviving hypoxia (RBC synthesis, blood vessel growth and anaerobic survival pathways)
Do proline hydroxylases have a high or low Km?
High which enables oxygen sensitive oxygen sensing over physiological ranges of PO2