Enzyme inhibition Flashcards
What is enzyme inhibition?
It is the decrease rate of an enzyme controlled reaction by another molecule called an inhibitor. An inhibitor combines with an enzyme and prevents it from forming an enzyme/substrate complex.
What is an inhibitor?
It is a molecule or ion bonds to an enzyme which slows down the rate of reaction.
What is competitive inhibition?
It is the reduction of rate in an enzyme controlled reaction by a molecule or ion which is complementary to the active site and similar to the substrate. Preventing the substrate from binding.
What are competitive inhibitors?
They have a molecular shape which is complimentary to the active site in similar to the substrate which means that they compete for the substrates position in the active site.
What happens to the rate of reaction when the substrate concentration increases in a competitive inhibition?
Increasing the substrate concentration reduces the effect of the inhibitor as there are more substrate molecules present. This means that the substrate has a greater chance of being bonded to the active site leaving fewer available for the inhibitor.
What would happen if the inhibited concentration was to increase?
If the inhibitors concentration was to increase the reaction would slow down as it would bind more to the active sites
What is a non-competitive inhibitor?
It is an atom, molecule or ion which reduces the rate of enzyme activity by binding at a allosteric site altering the shape of the active site preventing substrate from binding successfully.
What happens in non-competitive inhibition?
It is when the non-competitive inhibitors don’t compete for the active site against substrate. They affect the bonds within an enzyme molecule and affect its overall shape including the active site. This affects the rate as the substrate is no longer able to bind into the active site. So no enzyme-substrate complexes can form.
What happens if the noncompetitive inhibitor concentration increases?
The rate of reaction decreases as well as the final mass of product.
