Enzyme Inhibition Flashcards
What do competitive inhibitors do?
Bind to the active site of the enzyme
What do non-competitive inhibitors do?
Bind to the enzyme at a position other than the active site
What is the name of the site that the inhibitor binds to in non-competitive?
Allosteric site
What will adding a small concentration of inhibitor do?
Reduce the rate of reaction
What will adding an excess of an inhibitor to a solution do?
May Prevent the reaction completely
How does competitive inhibition reduce enzyme activity?
Occupies the active site so forms a physical barrier that prevents the enzyme-substrate complex forming.
Do competitive inhibitors have a more or less powerful inhibition effect?
Less powerful
Why does the rate of reaction eventually return to normal with competitive inhibition?
The competitors bind reversibly
What does the binding of a non-competitive inhibitor cause?
A conformational change in the enzyme
What causes the change in the enzyme from non-competitive?
Inhibitor binding Interacts with chemical groups
Why does the enzyme no longer function with non-competitive?
Alters the active site’s shape so it is no longer complimentary to the substrate
Why is it called non-competitive?
They don’t bind to the active site so there is no competition
Is non-competitive inhibition more or less powerful?
More powerful
Why is there an overall rate of reaction decrease using non-competitive?
The inhibitors bind permanently
What is end-product inhibition?
When the end product of a reaction acts as an inhibitor to an enzyme on a reaction pathway to reduce the amount of the end product produced
